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Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, Dibothriocephalus latus (Diphyllobothriidea)

Published online by Cambridge University Press:  08 March 2021

Martina Orosová Open the ORCID record for Martina Orosová [Opens in a new window] ,
Anna Marková ,
Irena Provazníková Open the ORCID record for Irena Provazníková [Opens in a new window] ,
Mikuláš Oros Open the ORCID record for Mikuláš Oros [Opens in a new window] ,
Alžbeta Radačovská ,
Zuzana Čadková  and
František Marec Open the ORCID record for František Marec [Opens in a new window]
Martina Orosová*
Affiliation:
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, Košice04001, Slovakia
Anna Marková
Affiliation:
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, Košice04001, Slovakia
Irena Provazníková
Affiliation:
Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Branišovská 31, České Budějovice37005, Czech Republic Faculty of Science, University of South Bohemia, Branišovská 1760, České Budějovice37005
Mikuláš Oros
Affiliation:
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, Košice04001, Slovakia
Alžbeta Radačovská
Affiliation:
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, Košice04001, Slovakia
Zuzana Čadková
Affiliation:
Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science Prague (CZU), Kamýcká 129, Prague16500
František Marec
Affiliation:
Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Branišovská 31, České Budějovice37005, Czech Republic
*
Author for correspondence: Martina Orosová, E-mail: [email protected]
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Abstract

The large-sized tapeworm Dibothriocephalus latus is known as the broad or fish-borne cestode of mammals that is capable to infect humans and cause diphyllobothriosis. Recently, molecular data on D. latus has been accumulating in the literature and a complete genome sequence has been published; however, little is known about the karyotype and chromosome architecture. In this study, an in-depth karyological analysis of 2 D. latus specimens was carried out. The plerocercoids originated from a perch caught in subalpine Lake Iseo (Italy) and the tapeworms were reared in hamsters. Both specimens contained cells with a highly variable number of chromosomes ranging from18 to 27. Nevertheless, the largest portion of mitotic figures (47%) showed a number corresponding to the triploid set, 3n = 27. Accordingly, the karyotype of the analyzed specimens consisted of 9 triplets of metacentric chromosomes. Fluorescence in situ hybridization (FISH) with the 18S rDNA probe clearly demonstrated the presence of 3 clusters of hybridization signals on the triplet of chromosome 7, thus confirming the triploid status of the specimens. FISH with a telomeric (TTAGGG)n probe confined hybridization signals exclusively to the terminal chromosomal regions, supporting the earlier findings that this repetitive motif is a conserved feature of tapeworm telomeres.

Keywords

18S rDNAparthenogenesispolyploidizationtelomerestriploid
Type
Research Article
Information
Parasitology , Volume 148 , Issue 7 , June 2021 , pp. 787 - 797
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

Present address: Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany.

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