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Molecular cloning of schistosome genes

Published online by Cambridge University Press:  23 August 2011

D. W. Taylor ,
J. S. Cordingley ,
D. W. Dunne ,
K. S. Johnson ,
W. J. Haddow ,
C. E. Hormaeche ,
V. Nene  and
A. E. Butterworth
D. W. Taylor
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
J. S. Cordingley
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
D. W. Dunne
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
K. S. Johnson
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
W. J. Haddow
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
C. E. Hormaeche
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
V. Nene
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
A. E. Butterworth
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
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Extract

As part of an integrated programme investigating human schistosomiasis, work which involves epidemiological surveys and detailed immunological studies as well as biochemical investigations, we have, over the last three years, been cloning schistosome genes in a variety of plasmid and lambda vector systems. In this lecture we present a review of some selected aspects of work primarily aimed at production of experimental vaccines against the disease but which, on a broader front, is also concerned with developmental regulation of gene expression around the parasite's life-cycle. Specifically, we are interested in cloning three groups of genes. First, those encoding surface antigens; second, those associated with sexual maturity and egg production; and third, antigens which may provide a basis for a specific immunodiagnostic test.

Type
Research Article
Information
Parasitology , Volume 92 , supplement S1: Symposia of the British Society for Parasitology Volume 23 Parasites and molecular biology: applications of new techniques , January 1986 , pp. S73 - S81
Copyright
Copyright © Cambridge University Press 1986

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References

REFERENCES

Butterworth, A. E. (1984). Cell-mediated damage to helminths. Advances in Parasitology 23, 143–235.CrossRefGoogle ScholarPubMed
Butterworth, A. E., Capron, M., Cordingley, J. S., Dalton, P. R., Dunne, D. W., Kariuki, H. C., Kimani, G., Koech, D., Mugambi, M., Ouma, J. H., Prentice, M. A., Richardson, B. A., Arap Siongok, T. K., Sturrock, R. F. & Taylor, D. W. (1985). Immunity after treatment of human schistosomiasis mansoni. II. Identification of resistant individuals, and analysis of their immune responses. Transactions of the Royal Society of Tropical Medicine and Hygiene 79, 393–408.CrossRefGoogle ScholarPubMed
Butterworth, A. E., Taylor, D. W., Veith, M. C., Vadas, M. A., Dessein, A., Sturrock, R. F. & Wells, E. (1982). Studies on the mechanisms of immunity in human schistosomiasis. Immuno-logical Reviews 61, 5–39.CrossRefGoogle ScholarPubMed
Capron, A., Dessaint, J. P., Capron, M., Joseph, M. & Pestel, J. (1980). Role of anaphylactic antibodies in immunity to schistosomes. American Journal of Tropical Medicine and Hygiene 29, 849–57.CrossRefGoogle ScholarPubMed
Capron, A., Dessaint, J. P., Capron, M., Joseph, M. & Torpier, G. (1982). Effector mechanisms of immunity to schistosomes and their regulation. Immunological Reviews 61, 41–66.CrossRefGoogle ScholarPubMed
Clements, J. D. & El-Mobshidy, S. (1984). Construction of a potential live oral bivalent vaccine for typhoid fever and cholera-Escherichia coli-related diarrhoeas. Infection Immunity 46, 564.CrossRefGoogle Scholar
Coedingley, J. S., Nene, V., Haddow, W. J. & Taylor, D. W. (1986). Identification by message selection of cDNA clones encoding antigens of Schistosoma mansoni. Molecular and Biochemical Parasitology 18, 73–88.CrossRefGoogle Scholar
Cordingley, J. S., Taylor, D. W., Dunne, D. W. & Butterworth, A. E. (1983). Clone banks of cDNA from the parasite Schistosoma mansoni: isolation of clones containing a potentially immuno-diagnostic antigen gene. Gene 26, 25–39.CrossRefGoogle Scholar
Ford, M. J., Bickle, Q. D., Taylor, M. G. & Andrews, B. J. (1984). Passive transfer of resistance and the site of immune-dependent elimination of the challenge infection in rats vaccinated with highly irradiated cercariae of Schistosoma mansoni. Parasitology 89, 461–82.CrossRefGoogle ScholarPubMed
Grunstein, M. & Hogness, D. S. (1975). Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences, USA 72, 3961–5.CrossRefGoogle ScholarPubMed
Grzych, J. M., Capron, M., Bazin, H. & Capron, A. (1982). In vitro and in vivo effector function of rat IgG2a monoclonal anti-S. mansoni antibodies. Journal of Immunology 129, 2739–43.CrossRefGoogle ScholarPubMed
Harn, D. A., Mitsuyama, M. & David, J. R. (1984). Schistosoma mansoni: Anti-egg monoclonal antibodies protect against cercarial challenge in vivo. Journal of Experimental Medicine 159, 1371–87.CrossRefGoogle ScholarPubMed
Hoeijmakers, J. H. J., Borst, P., Van Den Burg, J., Weissmann, C. & Cross, G. A. M. (1980). The isolation of plasmids containing DNA complementary to messenger RNA for variant surface glycoproteins of Trypanosoma brucei. Gene 8, 391–417.CrossRefGoogle ScholarPubMed
Hoiseth, S. K. & Stocker, B. A. D. (1981). Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines. Nature, London 291, 238.CrossRefGoogle ScholarPubMed
Ingolia, T. D. & Craig, E. A. (1982). Four small Drosophila heat shock proteins are related to each other and to mammalian α-crystallin. Proceedings of the National Academy of Sciences, USA 79, 2360–4.CrossRefGoogle ScholarPubMed
Johnson, K. S. (1986). Differential gene expression during development of Schistosoma mansoni. Ph.D. thesis, University of Cambridge.Google Scholar
Mangold, B. L. & Knopf, P. M. (1981). Host protective humoral immune responses to Schistosoma mansoni infections in the rat. Kinetics of hyperimmune serum-dependent sensitivity and elimination of schistosomes in a passive transfer system. Parasitology 83, 559–74.CrossRefGoogle Scholar
Moss, B., Smith, G. L., Gerin, J. L. & Purcell, R. H. (1984). Live recombinant vaccinia virus protects chimpanzees against hepatitis B. Nature, London 311, 67–9.CrossRefGoogle ScholarPubMed
Rousseauz-Prevost, R., Capron, M., Bazin, H. & Capron, A. (1978). IgE in experimental schistosomiasis. II. Quantitative determination of specific IgE antibodies against S. mansoni. A follow-up study of two strains of infected rats. Correlation with protective immunity. Immunology 35, 33–9.Google Scholar
Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463–7.CrossRefGoogle ScholarPubMed
Sher, A., Smithers, S. R., Mackenzie, P. & Broomfield, (1977). Schistosoma mansoni: Immuno-globulins involved in passive immunization of laboratory mice. Experimental Parasitology 41, 160–6.CrossRefGoogle Scholar
Smith, G. L., Mackett, M. & Moss, B. (1983). Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen. Nature, London 302, 490–5.CrossRefGoogle ScholarPubMed
Smith, M. A. & Clegg, J. A. (1985). Vaccination against Schistosoma mansoni with purified surface antigens. Science 227, 535–8.CrossRefGoogle ScholarPubMed
Smith, M. A., Clegg, J. A., Snary, D. & Trejdosiewicz, A. J. (1982). Passive immunization of mice against Schistosoma mansoni with an IgM monoclonal antibody. Parasitology 84, 83–9.CrossRefGoogle ScholarPubMed
Smith, M. A., Searle, P. F. & Williams, J. G. (1979). Characterisation of bacterial clones containing DNA sequences derived from X. laevis vitellogenin mRNA. Nucleic Acids Research 6, 487–506.CrossRefGoogle Scholar
Stanley, K. K. & Luzio, J. P. (1984). Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. The EMBO Journal 3, 1429–34.CrossRefGoogle ScholarPubMed
Taylor, D. W., Cordingley, J. S. & Butterworth, A. E. (1984). Immunoprecipitation of surface antigen precursors from Schistosoma mansoni messenger RNA in vitro translation products. Molecular and Biochemical Parasitology 10, 305–18.CrossRefGoogle ScholarPubMed
Taylor, D. W. & Wells, P. Z. (1984). Isolation and antigenic analysis of surface tegumental membranes from schistosomula of Schistosoma mansoni. Parasitology 89, 495–510.CrossRefGoogle Scholar
Vieira, J. & Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19, 259–68.CrossRefGoogle ScholarPubMed
Young, R. A. & Davis, R. W. (1983). Use of the lambda gt11 expression vector to do direct immunological screening on nitrocellulose filters. Proceedings of the National Academy of Sciences, USA 80, 1194–8.CrossRefGoogle Scholar