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Molecular characterization of Cryptosporidium parvum isolates from human cryptosporidiosis cases in Scotland

Published online by Cambridge University Press:  23 September 2014

A. P. DESHPANDE ,
B. L. JONES ,
L. CONNELLY ,
K. G. POLLOCK ,
S. BROWNLIE  and
C. L. ALEXANDER
A. P. DESHPANDE
Affiliation:
Clinical Microbiology, Greater Glasgow and Clyde NHS, Glasgow, UK
B. L. JONES
Affiliation:
Scottish Microbiology Reference Laboratories, Greater Glasgow and Clyde NHS, Level 5, New Lister Building, Glasgow Royal Infirmary, Alexandra Parade, Glasgow G31 2ER, UK
L. CONNELLY
Affiliation:
Scottish Microbiology Reference Laboratories, Greater Glasgow and Clyde NHS, Level 5, New Lister Building, Glasgow Royal Infirmary, Alexandra Parade, Glasgow G31 2ER, UK
K. G. POLLOCK
Affiliation:
Health Protection Scotland, NHS National Services Scotland, Glasgow, UK
S. BROWNLIE
Affiliation:
Health Protection Scotland, NHS National Services Scotland, Glasgow, UK
C. L. ALEXANDER*
Affiliation:
Scottish Microbiology Reference Laboratories, Greater Glasgow and Clyde NHS, Level 5, New Lister Building, Glasgow Royal Infirmary, Alexandra Parade, Glasgow G31 2ER, UK
*
* Corresponding author: Scottish Microbiology Reference Laboratories, Glasgow Scottish Parasite Diagnostic and Reference Section, Level 5, New Lister Building, Glasgow Royal Infirmary, Alexandra Parade, Glasgow G31 2ER. E-mail: [email protected]
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Summary

Cryptosporidium parvum (C. parvum) is one of the most prevalent protozoan pathogens responsible for inducing human and animal disease worldwide. In this study, the glycoprotein-60 (gp60) subtyping tool was employed to assess the molecular diversity of C. parvum from human feces throughout Scotland during potential outbreaks. Over a 24-month period, microscopy analysis revealed 1139 positive feces containing Cryptosporidium species with 256 identified by molecular methods specifically as C. parvum. Cryptosporidium parvum was shown to be more prevalent in rural areas of Scotland and subtyping of 87 isolates demonstrated the predominant family as IIa, which occurred in 94% (n = 82) of isolates. The IIaA15G1R1 subtype was most common, being isolated from 47% (n = 41) of Scottish human cases. Non-IIa strains constituted a total of 5 isolates and included subtypes from the IIc, IId and IIg families. This information contributes significantly to existing knowledge and understanding of C. parvum subtypes in Scotland which is vital in assisting with the management of future local and national outbreaks.

Keywords

Cryptosporidium parvummoleculardiversitysubtypesScotlandgp60
Type
Research Article
Information
Parasitology , Volume 142 , Issue 2 , February 2015 , pp. 318 - 325
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Copyright
Copyright © Cambridge University Press 2014 

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