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Mitochondrial membrane fluorescence and temperature adaptation in Schistocephalus solidus (Cestoda: Pseudophyllidea)

Published online by Cambridge University Press:  06 April 2009

R. W. Walker  and
J. Barrett
R. W. Walker
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed S Y23 3DA
J. Barrett
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed S Y23 3DA
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Extract

The fluorescent probe 1-anilino-8-naphthalene suiphonic acid (ANS) was used to investigate the effect of temperature on the physical state of the mitochondrial membranes of adult and larval schistocephalus solzdus together with that of their hosts Gasterosteus aculeatus and Gallus domesticus. Arrhenius plots of ANS/membrane fluorescence for S. solidus plerocercoids was linear over the temperature range 15 to 58 °C, while that for the adult was biphasic with a discontinuity at 39·9 °C. This was interpreted as a physical change which occurred in the adult membrane but not in the plerocercoid membrane and pointed to an alteration in membrane composition during infection. Gasierosteus aculeatus showed a linear Arrhenius plot for membrane fluorescence, irrespective of acclimation temperature. Gallus domesticus showed a discontinuity in the Arrhenius plot for membrane fluorescence at 46·9 °C, outside the normal physiological temperature range.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 1 , February 1985 , pp. 131 - 135
Copyright
Copyright © Cambridge University Press 1985

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References

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