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Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase

Published online by Cambridge University Press:  21 June 2010

ADRIANA SEIXAS ,
ANDRÉIA B. ESTRELA ,
JULIANA C. CEOLATO ,
EMERSON G. PONTES ,
FLÁVIO LARA ,
KATIA C. GONDIM  and
CARLOS TERMIGNONI
ADRIANA SEIXAS*
Affiliation:
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
ANDRÉIA B. ESTRELA
Affiliation:
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
JULIANA C. CEOLATO
Affiliation:
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
EMERSON G. PONTES
Affiliation:
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
FLÁVIO LARA
Affiliation:
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil Pavilhão Hanseníase, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil
KATIA C. GONDIM
Affiliation:
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
CARLOS TERMIGNONI
Affiliation:
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
*
*Corresponding author: Avenida Bento Gonçalves 9500, Prédio 43431, Sala 217, Campus do Vale, Caixa Postal 15005, CEP 91501-970, Porto Alegre, RS, Brazil. Tel: +55 51 33086082. Fax: +55 51 33087309. E-mail: [email protected]
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Summary

The tick Rhipicephalus (Boophilus) microplus is an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite's physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzyme involved in vitellin hydrolysis in R. microplus eggs. Here we show an association between VTDCE and vitellin in an additional site, apart from the active site. Our data also demonstrate cysteine endopeptidase activity in different tissues such as ovary, gut, fat body, salivary gland and female haemolymph, where it is controlled by a physiological inhibitor. In R. microplus female gut, VTDCE is localized in areas of protein synthesis and trafficking with the underlying haemolymph. VTDCE is also localized in the ovary basal region, in vesicle membranes of ovary pedicel cells and in oocyte cytosol. These results suggest that VTDCE plays a role in vitellin digestion during tick development.

Keywords

Rhipicephalus (Boophilus) micropluscysteine endopeptidasecysteine endopeptidase inhibitorhaemolymphvitellinVTDCE
Type
Research Article
Information
Parasitology , Volume 137 , Issue 12 , October 2010 , pp. 1819 - 1831
Copyright
Copyright © Cambridge University Press 2010

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