Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T12:24:45.375Z Has data issue: false hasContentIssue false

Linked metabolism of L-serine and L-alanine by Moniliformis moniliformis (Acanthocephala) in vitro

Published online by Cambridge University Press:  06 April 2009

P. F. V. Ward
Affiliation:
Department of Parasitology, The Molteno Institute, University of Cambridge, Downing Street, Cambridge CB2 3EE
D. W. T. Crompton
Affiliation:
Department of Parasitology, The Molteno Institute, University of Cambridge, Downing Street, Cambridge CB2 3EE

Summary

Experiments to investigate the linked metabolism of L-serine and L-alanine by Moniliformis moniliformis in vitro were carried out by incubating adult worms aerobically for 3 h at 37 °C in Tyrode’s solution containing L-[U-14C]alanine and other amino acids. When present in the medium alone, alanine was totally removed by the worms and metabolized almost entirely to ethanol and a compound identified as carbon dioxide. When present in the medium with serine and no other amino acids, alanine was largely metabolized as before but additional alanine, believed to originate from serine, was excreted. The same results were obtained with serine and 16 other amino acids in the incubation medium.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aist, S. & Riggs, R. D. (1969). Amino acids from Heterodera glycines. Journal of Nematology 1, 254–9.Google ScholarPubMed
Barrett, J. (1981). Biochemistry of Parasitic Helminths. London and Basingstoke: Macmillan.CrossRefGoogle Scholar
Crompton, D. W. T. & Walters, D. E. (1972). An analysis of the course of infection of Moniliformis dubius in rats. Parasitology 64, 517–23.CrossRefGoogle ScholarPubMed
Crompton, D. W. T. & Ward, P. F. V. (1984). Selective metabolism of L-serine by Moniliformis (Acanthocephala) in vitro. Parasitology 89, 133–44.CrossRefGoogle ScholarPubMed
Davey, R. A. & Bryant, C. (1969). The tricarboxylic acid cycle and associated reactions in Moniezia expansa (Cestoda). Comparative Biochemistry and Physiology 31, 503–11.CrossRefGoogle ScholarPubMed
Hochachka, P. W. & Mustafa, T. (1972). Invertebrate facultative anaerobiosis. Science 178, 1056–60.CrossRefGoogle ScholarPubMed
Körting, W. & Fairbairn, D. (1972). Anaerobic energy metabolism in Moniliformis dubius (Acanthocephala). Journal of Parasitology 58, 4550.CrossRefGoogle ScholarPubMed
Lackie, J. M. (1972). The course of infection and growth of Moniliformis dubius (Acanthocephala) in the intermediate host Periplaneta americana. Parasitology 64, 95106.CrossRefGoogle ScholarPubMed
Prescott, L. M. & Campbell, J. W. (1965). Phosphoenol-pyruvate carboxylase activity and glycogenesis in the flatworm Hymenolepis diminuta. Comparative Biochemistry and Physiology 14, 491511.CrossRefGoogle Scholar
Read, C. P., Rothman, A. H. & Simmons, J. E. (1963). Studies on membrane transport with special reference to host-parasite integration. Annals of the New York Academy of Sciences 113, 154205.CrossRefGoogle Scholar
Starling, J. A. (1985). Feeding, nutrition and metabolism. In Biology of the Acanthocephala (ed. Crompton, D. W. T. and Nickol, B. B.), pp. 125212. Cambridge: Cambridge University Press.Google Scholar
Tanaka, R. D. & MacInnis, A. J. (1980). Analyses of the pseudocoelomic fluid from Moniliformis dubius. Journal of Parasitology 66, 354–5.CrossRefGoogle ScholarPubMed
Ward, P. F. V. (1974). The metabolism of glucose by Haemonchus contortus in vitro. Parasitology 69, 175–90.CrossRefGoogle Scholar
Ward, P. F. V., Coadwell, W. J. & Huskisson, N. S. (1981). The glucose metabolism of adult Ostertagia circumcincta in vitro. Parasitology 82, 1722.CrossRefGoogle ScholarPubMed
Ward, P. F. V. & Crompton, D. W. T. (1969). The alcoholic fermentation of glucose by Moniliformis dubius (Acanthocephala), in vitro. Proceedings of the Royal Society of London, B 172, 6588.Google ScholarPubMed
Ward, P. F. V. & Huskisson, N. S. (1972). The metabolism of fluoroacetate in lettuce. The Biochemical Journal 130, 575–87.CrossRefGoogle Scholar
Ward, P. F. V. & Huskisson, N. S. (1978). The energy metabolism of adult Haemonchus contortus in vitro. Parasitology 77, 255–71.CrossRefGoogle ScholarPubMed
Ward, P. F. V. & Huskisson, N. S. (1980). The role of carbon dioxide in the metabolism of adult Haemonchus contortus in vitro. Parasitology 80, 7382.CrossRefGoogle Scholar