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Leishmania major H-line attenuated under pressure of gentamicin, induces a Th1 response which protects susceptible BALB/c mice against infection with virulent L. major

Published online by Cambridge University Press:  03 August 2009

HAMID DANESHVAR ,
RICHARD BURCHMORE ,
PAUL HAGAN  and
R. STEPHEN PHILLIPS
HAMID DANESHVAR*
Affiliation:
Medical School, University of Kerman, Kerman, Iran
RICHARD BURCHMORE
Affiliation:
Sir Henry Wellcome Function Genomics Facility, Institute of Biomedical and Life Science, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
PAUL HAGAN
Affiliation:
Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, UK
R. STEPHEN PHILLIPS
Affiliation:
Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, UK
*
*Corresponding author: Medical School, University of Kerman, Kerman, Iran. Tel: +98 341 2223016. Fax: +98 341 2112794. E-mail: [email protected]
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Summary

An attenuated line of Leishmania major (L. major H-line) has been established by culturing promastigotes in vitro under gentamicin pressure. A modification of the previously described method for the generation of attenuated L. major is described, giving rise to attenuated parasites after 8 rather than 12 subpassages. No lesions developed in BALB/c mice infected with L. major H-line, whereas L. major wild-type (WT) induced a Th2 like response with progressive lesions. Analysis of splenocyte IFN-γ and IL-4 production following stimulation with promastigotes shows that the L. major H-line preferentially induces Th1-like responses and possibly down-regulates Th2 responses in BALB/c mice. L. major H-line parasites remained localized in the skin and draining lymph node, whereas L. major WT parasites disseminated into the visceral organs of BALB/c mice. Mice infected with L. major H-line acquired some resistance against L. major WT. These results show that the attenuated cell line of L. major is not only avirulent but that it may also modulate the host immune response.

Keywords

Leishmania major gentamicin attenuated lineCD4+ Th1 response
Type
Research Article
Information
Parasitology , Volume 136 , Issue 11 , September 2009 , pp. 1243 - 1250
Copyright
Copyright © Cambridge University Press 2009

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