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Isolation and partial characterization of the tegumental outer membrane of adult Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

A. J. G. Simpson ,
M. D. Schryer ,
I. M. Cesari ,
W. H. Evans  and
S. R. Smithers
A. J. G. Simpson
Affiliation:
Divisions of Parasitology, National institute for Medical Research, Mill Hill, London NW7 1AA
M. D. Schryer
Affiliation:
Divisions of Parasitology, National institute for Medical Research, Mill Hill, London NW7 1AA
I. M. Cesari
Affiliation:
Divisions of Parasitology, National institute for Medical Research, Mill Hill, London NW7 1AA
W. H. Evans
Affiliation:
Divisions of BiochemistryNational institute for Medical Research, Mill Hill, London NW7 1AA
S. R. Smithers
Affiliation:
Divisions of Parasitology, National institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

Incubation of adult Schistosoma mansoni at 37 °C in chemically defined media caused the shedding of variable amounts of the parasite's tegument. In phosphate-buffered saline, pH 7·4, 37% of 125I-labelled wheat germ agglutinin which was attached to the schistosome surface was released within 5 min. When the released material was collected by centrifugation at 55000 g for 1 h, the pellet was seen to consist of components of the parasite tegument and contained 1% of the parasite protein and 6·2% of the 5′-AMPase activity. By monitoring the release of the gut enzyme, haemoglobinase, it was shown that predominantly tegumental material was released under these conditions. The material released from the parasite was separated by discontinuous sucrose gradient centrifugation into 4 major fractions and it was shown that a material banding at low density was highly enriched in vesicles of parasite outer membrane. Major polypeptides of apparent molecular weights 135000, 110000, 90000, 65000, 47000 and 40000 and glycoproteins of apparent molecular weights 135000, 120000, 65000, 63000 and 30–40000 were identified in this fraction. Comparison of the distribution of 5′-AMPase activity and the contents of each fraction as revealed by ultrastructural examination showed that this enzyme is a good marker for the tegumental outer membrane.

Type
Research Article
Information
Parasitology , Volume 83 , Issue 1 , August 1981 , pp. 163 - 177
Copyright
Copyright © Cambridge University Press 1981

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