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Intestinal pathology associated with Trichostrongylus colubriformis infection in sheep: vascular permeability and ultrastructure of the mucosa

Published online by Cambridge University Press:  06 April 2009

I. K. Barker
I. K. Barker
Affiliation:
Department of Veterinary Paraclinical Sciences, University of Melbourne, Veterinary Clinical Centre, Werribee, Vic. 3030, Australia
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Extract

Increased permeability of capillaries and venules, labelled with colloidal carbon, was observed in the superficial mucosa of the small intestine of sheep infected with Trichostrongylus colubriformis. The labelled vessels were restricted to infected portions of the gut, and the intensity of labelling appeared to be related to density of the worm population. Open junctions were seen between endothelial cells in permeable vessels. In moderately atrophic infected intestine, enterocytes were domed, had sparse, short, distorted microvilli and many polyribosomes. Tight junctions between enterocytes seemed to be intact and distended intercellular spaces were seen. In more severely atrophic mucosa, enterocytes had distended mitochondria and endoplasmic reticulum, bizarre shapes, and the continuity of the epithelial sheet was occasionally disrupted. There was oedema in the lamina propria and neutrophils were seen in migration to the lumen of the gut. The possible mechanisms inducing these changes and their significance in terms of plasma protein loss and intestinal function are discussed.

Type
Research Article
Information
Parasitology , Volume 70 , Issue 2 , April 1975 , pp. 173 - 180
Copyright
Copyright © Cambridge University Press 1975

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References

REFERENCES

Barbaro, J. F., (1972). Immediate hypersensitivity effector mechanisms I. In vitro reactions. In Immunity to Animal Parasites (ed. Soulsby, E. J. L.), pp. 131–53. New York: Academic Press.Google Scholar
Barger, I. A., Southcott, W. H., & Williams, V. J., (1973). Trichostrongylosis and wool growth. 2. The wool growth response of infected sheep to parenteral and duodenal cystine and cysteine supplementation. Australian Journal of Experimental Agriculture and Animal Husbandry 13, 351–9.CrossRefGoogle Scholar
Barker, I. K., (1973 a). A study of the pathogenesis of Trichostrongylus colubriformis infection in lambs with observations on the contribution of gastrointestinal plasma loss. International Journal for Parasitology 3, 743–57.CrossRefGoogle ScholarPubMed
Barker, I. K., (1973 b). Scanning electron microscopy of the duodenal mucosa of lambs infected with Trichostrongylus colubriformis. Parasitology 67, 307–14.CrossRefGoogle ScholarPubMed
Barker, I. K., (1974). The relationship of abnormal mucosal microtopography with distribution of Trichostrongylus colubriformis in the small intestines of lambs. International Journal for Parasitology 4, 153–63.CrossRefGoogle ScholarPubMed
Barker, I. K., (1975). The intestinal pathology associated with Trichostrongylus colubriformis infection in sheep: histology. Parasitology 70, 165–71.CrossRefGoogle ScholarPubMed
Bellamy, J. E. C., & Nielsen, N. O., (1974). A comparison between the active cutaneous arthus reaction and immune mediated enteroluminal neutrophil emigration in pigs. The Canadian Journal of Comparative Medicine 38, 193–202.Google ScholarPubMed
Bremner, K. C., (1969). Pathogenetic factors in experimental bovine oesophagostomosis. IV. Exudative enteropathy as a cause of hypoproteinemia. Experimental Parasitology 25, 382–94.CrossRefGoogle ScholarPubMed
Coop, R. L., & Angus, K. W., (1973). The effect of continuous doses of Trichostrongylus colubriformis larvae on serum electrolytes and intestinal enzyme activity in sheep. Parasitology 67, v–vi.Google Scholar
Coop, R. L., Angus, K. W., & Mapes, C. J., (1973). The effect of large doses of Nematodirus battus on the histology and biochemistry of the small intestine of lambs. International Journal for Parasitology 3, 349–61.CrossRefGoogle ScholarPubMed
Cotran, R. S., Suter, E. R., & Majno, G., (1967). The use of colloidal carbon as a tracer for vascular injury. A review. Vascular Diseases 4, 107–27.Google ScholarPubMed
Davenport, H. W., (1970). A hypothesis about protein-losing gastroenteropathy. Gastroenterology 58, 600–2.CrossRefGoogle ScholarPubMed
Dumonde, D. C., Wolstencroft, R. A., Panayi, G. S., Matthew, M., Morley, J., & Howson, W. T., (1969). ‘Lymphokines’: Non-antibody mediators of cellular immunity generated by lymphocyte activation. Nature, London 224, 38–42.CrossRefGoogle ScholarPubMed
Gallagher, N. D., Playoust, M. R., & Symons, L. E. A., (1971). Mechanism of fat malabsorption in rats infected with Nippostrongylus brasiliensis. Gut 12, 1007–10.CrossRefGoogle ScholarPubMed
Hurley, J. V., & McQueen, A., (1971). The response of the fenestrated vessels of the small intestine of rats to application of mustard oil. The Journal of Pathology 105, 21–9.CrossRefGoogle ScholarPubMed
Jarrett, W. F. H., Miller, H. R. P., & Murray, M., (1970). Immunological mechanisms in mucous membranes. In Resistance to Infectious Disease (ed. Dunlop, R. H. and Moon, H. W.), pp. 287–97. University of Saskatchewan: Saskatoon.Google Scholar
Jones, W. O., Rothwell, T. L. W., Dineen, J. K., & Griffiths, D. A., (1974). Studies on the role of histamine and 5-hydroxytryptamine in immunity against the nematode Trichostrongylus colubriformis. II. Amine levels in the intestine of infected guinea-pigs. International Archives of Allergy 64, 14–27.CrossRefGoogle Scholar
McLeay, L. M., Anderson, N., Bingley, J. B., & Titchen, D. A., (1973). Effects on abomasal function of Ostertagia circumcincta infections in sheep. Parasitology 66, 241–57.CrossRefGoogle ScholarPubMed
Miller, H. R. P., & Walshaw, R., (1972). Immune reactions in mucous membranes. IV. Histoehemistry of intestinal mast cells during helminth expulsion in rats. American Journal of Pathology 69, 195–208.Google Scholar
Miller, R. L., Reichgott, M. J., & Melmon, K. L., (1973). Biochemical mechanisms of generation of bradykinin by endotoxin. The Journal of Infectious Diseases 128: Supplement — Bacterial lipopolysaccharides: Chemistry, Biology and Clinical Significance of Endotoxins, pp. S144–56.Google ScholarPubMed
Murray, M., (1969). Structural changes in bovine ostertagiasis associated with increased permeability of the bowel wall to macromolecules. Gastroenterology 56, 763–72.CrossRefGoogle ScholarPubMed
Murray, M., (1972). Immediate hypersensitivity effector mechanisms. II. In vivo reactions. In Immunity to Animal Parasites, pp. 155–90 (ed. Soulsby, E. J. L.). New York: Academic Press.Google Scholar
Murray, M., Jarrett, W. F. H., Jennings, F. W., & Miller, H. R. P., (1971). Structural changes associated with increased permeability of parasitized mucous membranes to macromolecules. In Pathology of Parasitic Diseases (ed. Gaafar, S. M.), pp. 197–207, Lafayette Indiana: Purdue.Google Scholar
Olson, L. J., & Richardson, J. A., (1968). Intestinal malabsorption of D-glucose in mice infected with Trichinella spiralis. The Journal of Parasitology 54, 445–51.CrossRefGoogle ScholarPubMed
Rhodes, R. S., & Karnovsky, M. J., (1971). Loss of macromolecular barrier function associated with surgical trauma to the intestine. Laboratory Investigation 25, 220–9.Google ScholarPubMed
Rothwell, T. L. W., & Merritt, G. C., (1974). Acetylcholinesterase secretion by parasitic nematodes. IV. Antibodies against the enzyme in Trichostrongylus colubriformis-infected sheep. International Journal for Parasitology 4, 63–71.CrossRefGoogle ScholarPubMed
Rubin, W, (1971). The epithelial membrane of the small intestine. American Journal of Clinical Nutrition 24, 45–64.CrossRefGoogle ScholarPubMed
Symons, L. E. A., Gibbins, J. R., & Jones, W. O., (1971). Jejunal malabsorption in the rat infected by the nematode Nippostrongylus brasiliensis. International Journal for Parasitology 1, 179–87.CrossRefGoogle ScholarPubMed
Symons, L. E. A., & Jones, W. O., (1970). Nematospiroides dubius, Nippostrongylus brasiliensis, and Trichostrongylus colubriformis: Protein digestion in infected mammals. Experimental Parasitology 27, 496–506.CrossRefGoogle ScholarPubMed
Velo, G. P., Dunn, C. J., Giroud, J. P., Timsit, J., & Willoughby, D. A., (1973). Distribution of prostoglandins in inflammatory exudate. The Journal of Pathology 111, 149–58.CrossRefGoogle Scholar
Warshaw, A. L., Walker, W. A., Cornell, R., & Isselbacher, K. J., (1971). Small intestinal permeability to macromolecules. Transmission of horseradish peroxidase into mesenteric lymph and portal blood. Laboratory Investigation 25, 675–84.Google ScholarPubMed