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Inhibition of malarial invasion by intracellular antibodies against intrinsic membrane proteins in the red cell

Published online by Cambridge University Press:  06 April 2009

A. R. Dluzewski ,
K. Rangachari ,
M. J. A. Tanner ,
D. J. Anstee ,
R. J. M. Wilson  and
W. B. Gratzer
A. R. Dluzewski
Affiliation:
Medical Research Council Cell Biophysics Unit, King's College, Drury Lane, London WC2B 5RL
K. Rangachari
Affiliation:
Medical Research Council Cell Biophysics Unit, King's College, Drury Lane, London WC2B 5RL
M. J. A. Tanner
Affiliation:
Department of Biochemistry, University of Bristol, Medical School, Bristol BS8 1TD
D. J. Anstee
Affiliation:
South Western Regional Transfusion Centre, Southmead Road, Bristol BS10 5ND
R. J. M. Wilson
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
W. B. Gratzer
Affiliation:
Medical Research Council Cell Biophysics Unit, King's College, Drury Lane, London WC2B 5RL
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Summary

It has previously been shown that antibodies against the transmembrane proteins, band 3 and glycophorin A, inhibit entry of the merozoite into the red cell and, in the case of band 3, it was established that attachment of the parasite to the cell is not prevented. We have found that antibodies against the cytoplasmic domains of band 3 and of glycophorin A, when present in the interior of resealed ghosts of human red cells, also inhibit invasion by P. falciparum. It is inferred that attachment of the merozoite to the red cell causes structural effects that are transduced to the membrane cytoskeleton and the antibodies against transmembrane proteins interfere with the invasion sequence at this level.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 3 , December 1986 , pp. 427 - 431
Copyright
Copyright © Cambridge University Press 1986

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