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Inhibition of Entamoeba histolytica in vitro by specific antibody*

Published online by Cambridge University Press:  06 April 2009

Mitsuru Nakamura
Affiliation:
Department of Bacteriology, Montana State University, Missoula, and the Department of Microbiology, Boston University School of Medicine, Boston

Extract

Bacteria-free cultures of E. histolytica have been inhibited by specific and related antibodies in the antisera produced in rabbits. Maximal culture-inhibiting antibodies were produced when the rabbits were immunized with frozen and thawed, formalin-treated trophozoites injected intravenously. Human sera from subjects with current or past histories of amoebiasis also inhibited growth of amoebae in vitro.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1959

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References

REFERENCES

Buchman, E., Kullman, H. J. & Margonis, G. F. (1952). Evaluation of the complement-fixation test for amebiasis. Oastroenterology, 21, 391.CrossRefGoogle ScholarPubMed
Cole, B. A. & Kent, J. F. (1953). Immobilization of Endamoeba histolytica in vitro by anti-serum produced in the rabbit. Proc. Soc. Exp. Biol., N. Y., 83, 811.CrossRefGoogle Scholar
Dolkart, R. E., Halpern, B. & Cullen, J. (1951). The diagnosis of amebiasis, the role of the complement-fixation test, and the incidence of the disease in the Chicago area. J. Lab. Clin. Med. 38, 804.Google Scholar
Greif, R. L. (1947). Agglutination of Endamoeba histolytica cysts. Amer. J. Trop. med. 27, 131.CrossRefGoogle ScholarPubMed
Goldman, M. (1953). Cytochemical differentiation of Endamoeba histolytica and Endamoeba coli by means of fluorescent antibody. Amer. J. Hyg. 58, 319.Google ScholarPubMed
Goldman, M. (1954). Use of fluorescein-tagged antibody to identify cultures of Endamoeba histolytica and Endamoeba coli. Amer. J. Hyg. 59, 318.Google ScholarPubMed
Magath, T. B. & Meleney, H. E. (1940). Complement fixation reaction for amebiasis; comparative tests performed by two laboratories. Amer. J. Trop. Med. 20, 211.CrossRefGoogle Scholar
McDearman, S. C. & Dunham, W. B. (1952). Complement-fixation tests as an aid in the differential diagnosis of extra-intestinal amebiasis. Amer. J. Trop. Med. Hyg. 1, 182.CrossRefGoogle ScholarPubMed
Nakamura, M. (1955). Growth factors for Endamoeba histolytica. Proc. Soc. Exp. Biol., N. Y., 89, 680.CrossRefGoogle ScholarPubMed
Nakamura, M. & Baker, E. E. (1957). Agar diffusion precipitin technic for the detection of antibodies against Endamoeba histolytica. Bact. Proc. p. 95.Google Scholar
Paulson, M. & Andrews, J. (1938). Complement fixation in amebiasis. A comparative evaluation in clinical practice. Arch. Int. Med. 62, 562.CrossRefGoogle Scholar
Shaffer, J. G. & Balsam, T. (1954). Ability of Endamoeba histolytica to phagocytose red blood cells. Proc. Soc. Exp. Biol., N. Y., 85, 21.CrossRefGoogle ScholarPubMed