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In vitro suppression of segmentation in Echinococcus multilocularis with morphological transformation of protoscoleces into monozoic adults

Published online by Cambridge University Press:  06 April 2009

J. D. Smyth  and
Zena Davies
J. D. Smyth
Affiliation:
Department of Zoology & Applied Entomology, Imperial College, London, SW7 2BB
Zena Davies
Affiliation:
Department of Zoology & Applied Entomology, Imperial College, London, SW7 2BB
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Extract

When protoscoleces of Echinococcus multilocularis were cultured in vitro, under axenic conditions in either monophasic or diphasic media, segmentation was suppressed in most organisms, some 70–80% of which developed into unsegmented, monozoic forms with a complete set of sexually mature male and female genitalia. The most striking feature of monozoic worms was the large lateral swelling produced by the cirrus sac the effect being to produce organisms with an unusual asymmetric shape. Worms which did not become monozoic either (a) underwent some somatic growth, developed two sets of genitalia and became ‘pseudosogmented’, i.e. with the inter-proglottid membranes absent or poorly defined, or (b) became vesicular or abnormal. The mechanisms which could be involved in the suppression of somatic growth and the induction of the monozoic condition, are examined in terms of cell lineage. The possible significance of these results in understanding the evolution of the cestodes is discussed.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 1 , August 1975 , pp. 125 - 136
Copyright
Copyright © Cambridge University Press 1975

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