Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-26T00:30:13.039Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro hatching of Hymenolepis diminuta eggs in Tenebrio molitor extracts and in defined enzyme preparations

Published online by Cambridge University Press:  06 April 2009

R. C. Lethbridge
R. C. Lethbridge
Affiliation:
Department of Zoology, Imperial College, London, S. W.7*
Get access Rights & Permissions [Opens in a new window]

Extract

The results of several experiments that included variation of the pH and concentration of the extracts and analysis of the role of enzymes separated from midgut fluid by electrophoresis indicated that hatching involved two or more proteolytic enzymes that digested the cytoplasmic layer and embryophore. Amylases from T. molitor and other sources were shown to play no part in hatching.A defined medium based on analyses of midgut fluid and containing trypsin, chymotrypsin and peptidases was shown to digest the embryophore and effect the release of hexacanths in a time comparable to that obtained with T. molitor extracts. The specificity of the enzymes attacking the cytoplasmic layer remains unknown.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 3 , June 1972 , pp. 389 - 400
Copyright
Copyright © Cambridge University Press 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Berntzen, A. K., & Voge, M., (1965). In vitro hatching of oncospheres of four hymenolepidid cestodes. Journal of Parasitology 51, 235–42.CrossRefGoogle ScholarPubMed
Birk, Y., Harpaz, I., Ishaaya, I., & Bondi, A., (1962). Studies on the proteolytic activity of the beetles Tenebrio and Tribolium. Journal of Insect Physiology 8, 417–29.CrossRefGoogle Scholar
Cheng, T. C., & Dyckman, E., (1964). Sites of glycogen deposition in Hymenolepis diminuta during the growth phase in the rat host. Zeitschrift für Parasitenkunde 24, 49–64.Google Scholar
Dadd, R. H., (1956). Proteolytic activity of the midgut in relation to feeding in the beetles Tenebrio molitor L. and Dytiscus marginalis L. Journal of experimental Biology 33, 311–24.CrossRefGoogle Scholar
Fruton, J. S., & Simmonds, S., (1958). General Biochemistry. New York: John Wiley and Sons, Inc.Google Scholar
Gilmour, D., (1961). The Biochemistry of Insects. New York and London: Academic Press, Inc.Google Scholar
Hedrick, R. M., & Daugherty, J. W., (1957). Comparative histochemical studies on cestodes. I. The distribution of glycogen in Hymenolepis diminuta and Raillietina cesticillus. Journal of Parasitology 43, 497–504.CrossRefGoogle ScholarPubMed
Lethbridge, R. C., (1971 a). The hatching of Hymenolepis diminuta eggs and penetration of hexacanths in Tenebrio molitor beetles. Parasitology 62, 445–56.CrossRefGoogle ScholarPubMed
Lethbridge, R. C., (1971 b). The chemical composition and some properties of the egg layers in Hymenolepis diminuta eggs. Parasitology 63, 275–88.CrossRefGoogle ScholarPubMed
Lethbridge, R. C., (1971 c). An improved gradient centrifugation technic for the collection of Hymenolepis diminuta eggs from feces. Journal of Parasitology 57, 1140–2.CrossRefGoogle Scholar
McGeachin, R. L., & Pavord, W. C., (1962). Effect of protamine on hog pancreatic amylase. Proceedings of the Society for experimental Biology and Medicine 109, 556–8.CrossRefGoogle Scholar
Pence, D. B., (1970). Electron microscope and histoehemical studies on the eggs of Hymenolepis diminuta. Journal of Parasitology 56, 84–97.CrossRefGoogle Scholar
Ravin, H. A., (1954). A colourimetric micromethod for the estimation of chymotrypsin activity. Journal of Biological Chemistry 208, 1–16.CrossRefGoogle ScholarPubMed
Rockstein, M. (ed.) (1965). The Physiology of Insecta, vol. 2. New York and London: Academic Press, Inc.Google Scholar
Rybicka, K., (1967). Embryogenesis in Hymenolepis diminuta. II. Glycogen distribution in the embryos. Experimental Parasitology 20, 98–105.CrossRefGoogle ScholarPubMed
Siegelman, A. M., Carlson, A. S., & Robertson, T., (1962). Investigation of serum trypsin and related substances. I. The quantitative demonstration of trypsin-like activity in human blood serum by a micromethod. Archives of Biochemistry and Biophysics 97, 159–63.CrossRefGoogle Scholar
Smith, I., (1960). Chromatographic and Electrophoretic Techniques, vol. 2. London: Heinemann.Google Scholar
Voge, M., & Berntzen, A. K., (1961). In vitro hatching of oncospheres of Hymenolepis diminuta (Cestoda: Cyclophyllidea). Journal of Parasitology 47, 813–18.CrossRefGoogle ScholarPubMed
Wigglesworth, V. B., (1927). Digestion in the cockroach. II. The digestion of carbohydrates. Biochemical Journal 21, 797–811.CrossRefGoogle Scholar
Wigglesworth, V. B., (1928). Digestion in the cockroach. III. The digestion of proteins and fats. Biochemical Journal 22, 150–61.CrossRefGoogle Scholar