Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-26T00:41:01.633Z Has data issue: false hasContentIssue false
  • English
  • Français

The in vitro effect of hexachlorophene on some enzymes in the liver fluke, Fasciola hepatica L.

Published online by Cambridge University Press:  06 April 2009

Walborg Thorsell
Walborg Thorsell
Affiliation:
Department of Medical Chemistry, Royal Veterinary College, Stockholm 50, Sweden
Get access Rights & Permissions [Opens in a new window]

Extract

The effect of hexachlorophene, a fasciolicide, on some enzyme systems in the liver fluke was studied. The enzymes were adenosine triphosphatase, succinate oxidase and cholinesterase. Adenosine triphosphatase was activated, whereas succinate oxidase and cholinesterase were inhibited when homogenized flukes were incubated in some concentrations of hexachlorophene. Of the three enzyme systems studied in flukes killed by hexachlorophene, only the succinate system showed reduced activity, whereas the other two showed almost normal activities. The study indicates that biochemical changes are associated with the effect of hexachlorophene on the liver fluke.

Thanks are due to Dr C. Grant for critical examination of the language, to Miss Maarja Kippar for valuable help with the experiments and to Mrs Astrid Holmström for the typing of the manuscript.

This work was supported by grants A 1108/B 904 and A 1635/B 1299 from Jordbrukets Forskningsråd.

Type
Research Article
Information
Parasitology , Volume 57 , Issue 4 , November 1967 , pp. 665 - 671
Copyright
Copyright © Cambridge University Press 1967

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Albert, A. (1965). Selective Toxicity, 3rd ed.London: Methuen and Co. Ltd.Google ScholarPubMed
Allen, J. M. (1962). The Molecular Control of Cellular Activity. New York: McGraw-Hill.Google Scholar
Augustinsson, K. B. (1948). Cholinesterases. Acta physiol. scand. 15, suppl, 52.Google Scholar
Bauer, E. L. (1960). A Statistical Manual for Chemists. New York and London: Academic Press.Google Scholar
Bečejac, S., Lui, A., Krvavica, S. & Kralj, N. (1964). Acetylcholinesterase and butyrocholinesterase activity in the lancet fluke (Dicrocoelium lanceatum Rudolphi). Vet. Arh. 34, 87–9.Google Scholar
Benk, E. (1957). Über den Nachweis von Hexachlordioxydiphenylmethan. Seifen-Öle-Fette-Wachse 13, 377.Google Scholar
Björkman, N. & Thorsell, W. (1964). On the fine structure and resorptive function of the cuticle of the liver fluke, Fasciola hepatica L. Expl Cell Res. 33, 319–29.CrossRefGoogle Scholar
v. Brand, T. (1966). Biochemistry of Parasites. New York: Academic Press.Google Scholar
Broda, E. (1964). Molekulare Bioenergetik. Naturw. Rdsch. Stuttg. 17, 293–99.Google Scholar
Bryant, C. & Williams, J. P. G. (1962). Some aspects of the metabolism of the liver fluke, Fasciola hepatica L. Expl Parasit. 12, 372–6.CrossRefGoogle Scholar
Chance, M. R. A. & Mansour, T. E. (1953). A contribution to the pharmacology of movement in the liver fluke. Br. J. Pharmac. Chemother. 8, 134–8.CrossRefGoogle Scholar
Colowick, S. P. & Kaplan, N. O. (1955, 1957, 1962). Methods in Enzymology, vols. 1, 4 and 5. New York: Academic Press.Google Scholar
Czupryna, A., Ingrid-Osiňska, K.et al. (1965). The level of some energy rich compounds and ATP-ase activity during development. Pr. i Mater. Nauk. IMD. 6, 139–47.Google Scholar
Dawes, B. (1954). Maintenance in vitro of Fasciola hepatica L. Nature, Lond. 174, 654–5.CrossRefGoogle Scholar
Dorsman, W. (1962), Contribution to the Control of Fascioliasis. Thesis, University of Amsterdam. Meppel. N. V. Noord-Nederlandse Drukkerij.Google Scholar
Gould, B. S., Bosniak, M. A., Neidleman, S. & Gatt, S. (1953). Effect of hexachlorophene and related bisphenolie compounds on the dehydrogenase and cytochrome system of Bacillus subtilis and Escherichia coli. Archs Biochem. Biophys. 44, 284–97.CrossRefGoogle ScholarPubMed
Gould, B. S., Frigerio, N. A. & Lerowitz, W. B. (1955). The action of bisphenolie compounds on succinoxidase, cytochrome c oxidase and lactic dehydrogenase of animal tissue. Archs Biochem. Biophys. 56, 476–86.CrossRefGoogle ScholarPubMed
Mahler, W. (1954). The ionization of certain bisphenols. J. Am. chem. Soc. 76, 3920–21.CrossRefGoogle Scholar
Pearse, A. G. E. (1961). Histochemistry, 2nd ed.London: Churchill Ltd.Google ScholarPubMed
Rouiller, C. (1964). The Liver, vol. 2. New York: Academic Press.Google Scholar
Thorsell, W. (1963). Biochemical studies on the liver fluke, Fasciola hepatica L. Acta chem. scand. 17, 884.CrossRefGoogle Scholar
Thorsell, W. & Appelgren, L.-E. (1966). Distribution and fate of C14-hexachlorophene in liver flukes, Fasciola hepatica L. Helminthologia (in the Press).Google Scholar
Thorsell, W. & Björkman, N. (1966). In vitro studies on the effect of hexachlorophene and its dimethylether on the liver fluke, Fasciola hepatica L. Z. ParasitKde. 28, 116–24.Google ScholarPubMed