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Immunostaining of spores and plasmodia of disparate myxozoan genera with comments on the properties of the sporular mucus envelope

Published online by Cambridge University Press:  03 February 2006

D. J. MORRIS ,
K. MOLNÁR ,
M. LONGSHAW  and
A. ADAMS
D. J. MORRIS
Affiliation:
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
K. MOLNÁR
Affiliation:
Veterinary Medical Research Institute, Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary
M. LONGSHAW
Affiliation:
Centre for Environment, Fisheries and Aquaculture Science (CEFAS), Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK
A. ADAMS
Affiliation:
Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK
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Abstract

Species of the phylum Myxozoa are common parasites of fish and can cause severe losses in cultured species. Although a number of myxozoan life-cycles have now been elucidated, little is known about the biology of these organisms in the fish host. Monoclonal antibody B4 raised to the myxozoan Tetracapsuloides bryosalmonae has been previously noted to react with a number of species infecting fish kidney. We present the results of a survey of 55 myxosporean species that determined that this antibody detects an antigen on the spore surface of 33 of these species in the genera Myxobolus, Sphaerospora and Thelohanellus. However, there appears to be no clear relationship between those spores that contain the MAb B4 reactive antigen and the host or organ in which they are detected. The antigen appears to be synthesized in the plasmodial cytoplasm and is intimately associated with the surface of the spore capsules and, where present, the mucus envelope. The nature of this envelope is further discussed in relation to its formation and distinctive properties.

Keywords

MyxozoaMicrosporidiaimmunohistochemistrymucus envelope
Type
Research Article
Information
Parasitology , Volume 132 , Issue 6 , June 2006 , pp. 781 - 790
Copyright
2006 Cambridge University Press

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