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Immunoelectron microscopical studies of regulatory peptides in the nervous system of the monogenean parasite, Diclidophora merlangi

Published online by Cambridge University Press:  06 April 2009

G. P. Brennan ,
D. W. Halton ,
A. G. Maule ,
C. Shaw ,
C. F. Johnston ,
S. Moore  and
I. Fairweather
G. P. Brennan
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology and Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, UK
D. W. Halton*
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology and Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, UK
A. G. Maule
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology and Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, UK
C. Shaw
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, UK
C. F. Johnston
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, UK
S. Moore
Affiliation:
Peninsula Laboratories Europe Ltd, Merseyside WA9 3AJ UK
I. Fairweather
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology and Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, UK
*
*Reprint requests to: Professor D. W. Halton, School of Biology and Biochemisty, The Queen's University, Belfast BT7 1NN, Northern Ireland, UK.
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Summary

Specific antisera, directed against the highly conserved C-terminal hexapeptide amide of mammalian pancreatic poly-peptide (PP) and the invertebrate peptide FMRFamide, have been used in conjunction with post-embedding, IgG-conjugated colloidal gold immunostaining to demonstrate peptide immunoreactivity at subcellular level in the nervous system of adult Diclidophora merlangi. Gold labelling revealed that immunoreactivity for PP and FMRFamide was localized exclusively in dense-cored vesicles occupying the majority of axons in the central nervous system. Double-labelling demonstrated an apparent co-localization of PP and FMRFamide in the same dense-cored vesicles. Antigen pre-absorption experiments indicated cross-reactivity of the two antisera as unlikely, and that some if not all of the PP/FMRFamide immunostaining in the parasite was due to a neuropeptide F-like peptide.

Keywords

platyhelminthDiclidophora merlangimonogenean parasitenervous systemregulatory peptidesimmuno-gold labelling.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 2 , February 1993 , pp. 171 - 176
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Curry, J., Shaw, C., Johnston, C. F., Thim, L. & Buchanan, K. D. (1992). Neuropeptide F: primary structure from the turbellarian Artioposthia triangulata. Comparative Biochemistry and Physiology 101C, 269–74.Google ScholarPubMed
Fairweather, I. & Halton, D. W. (1991). Neuropeptides in platyhelminths. Parasitology 102, S77–S92.CrossRefGoogle ScholarPubMed
Halton, D. W. & Arme, C. (1971). In vitro technique for detecting tegument damage in Diclidophora merlangi: possible screening method for selection of undamaged tissues or organisms prior to physiological investigation. Experimental Parasitology. 30, 54–7.CrossRefGoogle ScholarPubMed
Halton, D. W., Fairweather, L., Shaw, C. & Johnston, C. F. (1990). Regulatory peptides in parasitic platyhelminths. Parasitology Today 6, 284–90.CrossRefGoogle ScholarPubMed
Halton, D. W., Shaw, C., Maule, A. C., Johnston, C. F. & Fairweather, I. (1992). Peptidergic messengers: a new perspective of the nervous system of parasitic platyhelminths. Journal of Parasitology 78, 179–93.CrossRefGoogle ScholarPubMed
Leung, P. S., Shaw, C., Maule, A. C., Thim, L., Johnston, C. F. & Irvine, G. B. (1992). A molluscan neuropeptide F. Regulatory Peptides 35, 242.Google Scholar
Magee, R. M., Fairweather, I., Shaw, C., McKillop, J. M., Montgomery, W. I., Johnston, C. F. & Halton, D. W. (1991). Quantification and partial characterisation of regulatory peptides in the liver fluke, Fasciola hepatica, from different mammalian hosts. Comparative Biochemistry and Physiology 99C, 201–7.Google ScholarPubMed
Maule, A. G., Halton, D. W., Johnston, C. F., Shaw, C. & Fairweather, I. (1990). The serotoninergic, cholinergic and peptidergic components of the nervous system in the monogenean parasite, Diclidophora merlangi: a cytochemical study. Parasitology 100, 255–73.CrossRefGoogle ScholarPubMed
Maule, A. G., Shaw, C., Halton, D. W., Johnston, C. F. & Fairweather, I. (1992). Immunochemical and chromatographic analyses of a neuropeptide from the monogenean parasite, Diclidophora merlangi: evolutionary aspects of the neuropeptide Y superfamily. Comparative Biochemistry and Physiology 102C, 517–22.Google Scholar
Maule, A. G., Shaw, C., Halton, D. W., Johnston, C. F., Fairweather, I. & Buchanan, K. D. (1989). Tachykinin immunoreactivity in the parasitic flatworm Diclidophora merlangi and its fish host the whiting (Merlangius merlangus): radioimmunoassay and chromatographic characterization using region-specific substance P and neurokinin A antisera. Comparative Biochemistry and Physiology 94C, 533–41.Google ScholarPubMed
Maule, A. G., Shaw, C., Halton, D. W., Thim, L., Johnston, C. F., Fairweather, I. & Buchanan, K. D. (1991). Neuropeptide F: a novel parasitic flatworm regulatory peptide from Moniezia expansa (Cestoda: Cyclophyllidea). Parasitology 102, 309–16.CrossRefGoogle Scholar
McKay, D. M., Shaw, C., Halton, D. W., Johnston, C. F., Fairweather, I. & Buchanan, K. D. (1990). Mammalian regulatory peptide immunoreactivity in the trematode parasite Haplometra cylindracea and the lung of its frog host, Rana temporaria: comparative chromatographic characterisation using reverse-phase high-performance liquid chromatography. Comparative Biochemistry and Physiology 96C, 345–51.Google ScholarPubMed
McKay, D. M., Fairweather, I., Johnston, C. F., Shaw, C. & Halton, D. W. (1991). Immunocytochemical and radioimmunometrical demonstration of serotonin- and neuropeptide-immunoreactivities in the adult rat tapeworm, Hymenolepis diminuta (Cestoda, Cyclophyllidae). Parasitology 103, 275–89.CrossRefGoogle Scholar
Skuce, P. J., Johnston, C. F., Fairweather, I., Halton, D. W., Shaw, C. & Buchanan, K. D. (1990). Immunoreactivity to the pancreatic polypeptide family in the nervous system of the adult human blood fluke, Schistosoma mansoni. Cell and Tissue Research 261, 573–81.CrossRefGoogle Scholar