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Immunodiagnosis of taeniasis by coproantigen detection

Published online by Cambridge University Press:  06 April 2009

J. C. Allan ,
G. Avila ,
J. Garcia Noval ,
A. Flisser  and
P. S. Craig
J. C. Allan
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
G. Avila
Affiliation:
Instituto de Investigaciones Biomedicas, Apartado Postal 70228, Ciudad Universitaria, 04150, Mexico D.F.
J. Garcia Noval
Affiliation:
Centro de Investigaciones de Ciencias de la Salud, Universidad de San Carlos de Guatemala, Guatemala City, Guatemala C.A.
A. Flisser
Affiliation:
Instituto de Investigaciones Biomedicas, Apartado Postal 70228, Ciudad Universitaria, 04150, Mexico D.F.
P. S. Craig
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
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Summary

Immunodiagnostic tests for Taenia-specific faecal antigen based on polyclonal rabbit antisera against Taenia saginata or Taenia solium proglottid extracts in capture-type ELISA assays have been developed. Taenia-specific antigen was detected in detergent-solubilized faecal extracts from T. solium- and T. saginata-infected hosts. Coproantigen from T. solium-infected hamsters did not cross-react with faeces from rodents infected with Hymenolepis diminuta, H. citelli, H. micro-stoma, Necator americanus, Strongyloides ratti or Nematospiroides dubius and faeces from uninfected animals. When the T. saginata-capture ELISA was tested with faecal samples positive for T. solium antigen, no cross-reactions were obtained. However, faecal samples from humans infected with T. solium or T. saginata, including some with extremely low egg counts, were cross-reactive by either test. Nevertheless, considerably higher O.D. values were obtained with stool samples from Taenia patients compared to Hymenolepis nana-infected or uninfected individuals. Two individuals, infected with Taenia sp. and positive for coproantigens by ELISA, became antigen-negative 6 days after treatment with Niclosamide. The possibility of developing species-specific immunodiagnostic tests for human taeniasis through coproantigen detection is discussed.

Keywords

immunodiagnosticcoproantigenTaenia saginataTaenia soliumgolden hamster
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 473 - 477
Copyright
Copyright © Cambridge University Press 1990

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References

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