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Immunization of cattle using varying infective doses of Theileria parva lawrencei sporozoites derived from an African buffalo (Syncerus caffer) and treatment with buparvaquone

Published online by Cambridge University Press:  06 April 2009

J. J. Mutugi ,
A. S. Young ,
A. C. Maritim ,
A. Linyonyi ,
S. K. Mbogo  and
B. L. Leitch
J. J. Mutugi
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
A. S. Young
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
A. C. Maritim
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
A. Linyonyi
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
S. K. Mbogo
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
B. L. Leitch
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
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Summary

A Theileria parva lawrencei isolate in the form of a sporozoite stabilate, derived by feeding clean Rhipicephalus appendiculatus nymphal ticks on an African buffalo (Syncerus caffer) captured in the Laikipia District, Kenya, was inoculated into groups of cattle at dilutions between 100 and 10-3. Groups of 3 cattle infected with 1 ml inocula at 100, 10-1 and 10-2 dilutions were treated with 2·5 mg/kg body weight of buparvaquone on day 0 and similar groups were left untreated to act as controls. An additional group, given 100 dilution of the stabilate, was treated with buparvaquone on day 8 post-inoculation. It was found that all control cattle inoculated with the stabilate at dilutions between 100 and 10-2 became infected, but only 2 out of 3 cattle developed patent infections at 10-3 dilution. All 3 control cattle receiving 100 dilution died of theileriosis, 2 at 10-1 and 10-2 dilutions, and 1 at 10-3 dilution died. Buparvaquone treatment on day 0 at 100 dilution resulted in the survival of 2 of 3 cattle and all the cattle at 10-1 and 10-2 dilutions. All the surviving cattle eventually developed a significant serological response against T. parva in the indirect fluorescent antibody test, except 1 in the 10-3 dilution group, and were immune to homologous challenge when tested 3 months later with a lethal inoculum of stabilate, except 2 cattle in the 10-3 dilution group. As a result of a theileriosis problem at about day 60 after inoculation in 2 cattle given 10-2 dilution of stabilate and buparvaquone treatment on day 0, an additional 5 cattle were given 10-2 dilution of stabilate and developed a good immunity after buparvaquone treatment. None was shown to develop the carrier state. Treatment with buparvaquone on day 8 after infection with 100 dilution of stabilate was not successful since 2 died. The stabilate used was shown to produce reproducible infection in cattle at different dilutions.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 2 , April 1988 , pp. 391 - 402
Copyright
Copyright © Cambridge University Press 1988

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References

REFERENCES

Barnett, S. F. & Brocklesby, D. W. (1966). Passage of ‘Theileria lawrencei (Kenya)’ through cattle. British Veterinary Journal 122, 396–409.Google Scholar
Brocklesby, D. W. (1964). Parasites of the family Theileridae of the African buffalo occurring in East Africa. D.V.M. thesis, University of Zurich.Google Scholar
Burridge, M. J. & Kimber, C. D. (1972). The indirect fluorescent antibody test for experimental East Coast fever (Theileria parva infection of cattle). Evaluation of a cell culture schizont antigen. Research in Veterinary Science 13, 451–5.Google Scholar
Burridge, M. J. & Ondeke, G. M. (1973). Theileria lawrencei: infection in Indian water buffalo, Bubalus bubalis. Experimental Parasitology 34, 257–61.Google Scholar
Conrad, P. A., Stagg, D. A., Grootenhuis, J. G., Irvin, A. D., Newson, J., Njamunggeh, R. E. G., Rossiter, P. B. & Young, A. S. (1987). Isolation of theilerial parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies. Parasitology 94, 413–23.Google Scholar
Dhar, S., Malhotra, D. V., Chandra, B. & Gautam, O. P. (1987). Chemoimmunoprophylaxis with buparvaquone against theileriosis in Neonatal calves. Veterinary Record 120, 375.Google Scholar
Dolan, T. T. (1985). The choice of drug for infection and treatment immunization against East African Theileriosis. In Immunization against Theileriosis in Africa (ed. Irvin, A. D.), pp. 100–3. Nairobi: ILRAD.Google Scholar
Dolan, T. T. (1986). Chemotherapy of East Coast fever. The long-term growth rate, carrier state and disease manifestations of parvaquone-treated cattle. Journal of Comparative Pathology 96, 137–45.Google Scholar
Dolan, T. T., Linyonyi, A., McHardy, N., Bond, A. L. & Clampitt, R. B. (1987). Parvaquone treatment of Theileria parva parva at intervals after infection. Research in Veterinary Science (in the Press).Google Scholar
Dolan, T. T., Linyonyi, A., Mbogo, S. K. & Young, A. S. (1984). A comparison of long-acting oxytetracycline and parvaquone for immunization against East Coast Fever by infection and treatment. Research in Veterinary Science 37, 175–8.Google Scholar
Fawcett, D. W., Young, A. S. & Leitch, B. L. (1985). Sporogony in Theileria (Apicomplexa: Theileriidae): a comparative ultrastructural study. Journal of Submicroscopic Cytology 17, 192–208.Google Scholar
Grootenhuis, J. G., Leitch, B. L., Stagg, D. A., Dolan, T. T. & Young, A. S. (1987). Experimental induction of Theileria parva lawrencei carrier state in an African buffalo (Syncerus caffer). Parasitology 94, 425–31.Google Scholar
Grootenhuis, J. G. & Young, A. S. (1981). The importance of wild life in the epidemiology of theileriosis. In Wildlife Disease Research and Economic Development, (ed. Karstad, L., Nestel, B. and Graham, M.), pp. 33–9. Ottawa: IDRC.Google Scholar
Mason, I. L. & Maule, J. P. (1960). The Indigenous Livestock of East and Central Africa. Farnham Royal: Commonwealth Agricultural Bureaux.Google Scholar
McHardy, N., Haigh, A. J. B. & Dolan, T. T. (1976). Chemotherapy of Theileria parva infection. Nature, London 261, 698–9.Google Scholar
McHardy, N. & Wekesa, L. S. (1985). Buparvaquone (BW720C) – a new antitheilerial naphthoquinone; its role in the therapy and prophylaxis of theileriosis. In Immunization against Theileriosis in Africa, (ed. Irvin, A. D.), p. 88. Nairobi: ILRAD.Google Scholar
McHardy, N., Wekesa, L. S., Hudson, A. T. & Randall, A. W. (1985). Antitheilerial activity of BW720C (buparvaquone): a comparison with parvaquone. Research in Veterinary Science 39, 29–33.Google Scholar
Moll, G., Lohding, A. & Young, A. S. (1984). Epidemiology of theileriosis in the Trans-Mara Division, Kenya. Husbandry and disease background and preliminary observation on theileriosis in calves. Preventive Veterinary Medicine 2, 801–34.Google Scholar
Moll, G., Lohding, A., Young, A. S. & Leitch, B. L. (1986). Epidemiology of theileriosis in calves in an endemic area of Kenya. Veterinary Parasitology 19, 255–73.Google Scholar
Morzaria, S. P., Irvin, A. D., Taracha, M., Spooner, P. R., Voigt, W. P., Fujinanga, T. & Katende, J. (1987). Immunization of cattle against East Coast fever. The use of selected stock of Theileria parva for immunization of cattle exposed to field challenge. Veterinary Parasitology 23, 23–41.Google Scholar
Mutugi, J. J., Young, A. S., Maritim, A. C., Ndungu, S. G., Stagg, D. A., Grootenhuis, J. G. & Leitch, B. L. (1987). Immunization of cattle against Theileriosis using varying doses of Theileria parva lawrencei and T. parva parva sporozoites and oxytetracycline treatments. International Journal for Parasitology (in the Press).Google Scholar
Neitz, W. O. (1955). Corridor Disease: a fatal form of bovine theileriosis encountered in Zululand. Bulletin of Epizootic Disease in Africa 3, 121–3.Google Scholar
Neitz, W. O. (1957). Theileriosis, gonderioses and cytauxzoones, a review. Onderstepoort Journal of Veterinary Research 27, 275–430.Google Scholar
Radley, D. E. (1978). Chemoprophylatic immunization of cattle against theileriosis. In Research on Tick-borne Diseases and Tick Control. FAO Technical Report No. 1 AG: DP.RAF/67/677 UNDP/FAO, Rome.Google Scholar
Radley, D. E. (1981). Infection and treatment method of immunization. In Advances in the Control of Theileriosis (ed. Irvin, A. D., Cunningham, M. P. and Young, A. S.), pp. 227–37. The Hague: Martinus Nijhoff Publishers.Google Scholar
Uilenberg, G. (1981). Theilerial species of livestock. In Advances in the Control of Theileriosis (ed. Irvin, A. D., Cunningham, M. P. and Young, A. S.), pp. 1–39. The Hague: Martinus Nijhoff Publishers.Google Scholar
Young, A. S. (1985). Immunization of cattle against Theileriosis in the Trans-Mara Division, Kenya. In Immunization against Theileriosis in Africa (ed. Irvin, A. D.), pp. 104–9. Nairobi: ILRAD.Google Scholar
Young, A. S., Branagan, D., Brown, C. G. D., Burridge, M. J., Cunningham, M. P. & Purnell, R. E. (1973 a). Preliminary observations on a theilerial species pathogenic to cattle isolated from buffalo (Syncerus caffer) in Tanzania. British Veterinary Journal 129, 382–9.Google Scholar
Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Kirimi, I. M. & Irvin, A. D. (1973 b). Observations on the cross-immunity between Theileria lawrencei (Serengeti) and Theileria parva (Muguga) in cattle. International Journal for Parasitology 3, 723–8.Google Scholar
Young, A. S., Brown, C. G. D., Burridge, M. J., Grootenhuis, J. G., Kanhai, G. K., Purnell, R. E. & Stagg, D. A. (1978 a). The incidence of theilerial parasites in East African buffalo (Syncerus caffer). Tropenmedizin und Parasitologie 29, 281–8.Google Scholar
Young, A. S., Brown, C. G. D., Cunningham, M. P. & Radley, D. E. (1978 b). Evaluation of immunization of cattle against Theileria lawrencei. In Tick-borne Disease and their Vectors (ed. Wilde, J. K. H.), pp. 292–6. Edinburgh: Centre for Tropical Veterinary Medicine.Google Scholar
Young, A. S., Dolan, T. T., Moll, G., Lohding, A., Leitch, B. L., Grootenhuis, J. G., Groocock, C. M. & De Castro, J. J. (1988 a). Immunization of introduced cattle against theileriosis in an endemic area of Kenya. Infection and treatment immunization of Boran (Bos indicus) cattle using parvaquone. Veterinary Parasitology (in the Press).Google Scholar
Young, A. S. & Grootenhuis, J. G. (1985). The influence of wildlife on the immunization of cattle theileriosis in East Africa. In Immunization against Theileriosis in Africa (ed. Irvin, A. D.), pp. 104–9. Nairobi: ILRAD.Google Scholar
Young, A. S., Leitch, B. L., Grootenhuis, J. G., Dolan, T. T., Mbogo, S. K., Ndungu, S. G. & De Castro, J. J. (1988 b). Immunization of introduced cattle against theileriosis in an endemic area of Kenya. Evaluation of infection and treatment methods using Sahiwal/Friesian cross cattle (Bos taurus/B. indicus). Preventive Veterinary Medicine (in the Press).Google Scholar
Young, A. S. & Purnell, R. E. (1973). Transmission of Theileria lawrencei (Serengeti) by the ixodid tick, Rhipicephalus appendiculatus. Tropical Animal Health and Production 5, 146–52.Google Scholar
Young, A. S. & Leitch, B. L. (1981). Epidemiology of East Coast fever. Some effects of temperature on the development of Theileria parva in its tick vector, Rhipicephalus appendiculatus. Parasitology 83, 199–211.Google Scholar