Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T00:38:05.987Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunity to Schistosoma mansoni in congenitally athymic, irradiated and mast cell-depleted rats

Published online by Cambridge University Press:  06 April 2009

M. J. Ford ,
Q. D. Bickle  and
M. G. Taylor
M. J. Ford
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
Q. D. Bickle
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
M. G. Taylor
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
Get access Rights & Permissions [Opens in a new window]

Summary

Immunity to Schistosoma mansoni was investigated in congenitally athymic (Nu/Nu) rats, irradiated rats and in mast cell-depleted rats. Nu/Nu rats failed to develop significant resistance following vaccination with irradiated cercariae, although Nu/Nu recipients of serum from vaccinated Fischer rats (VRS) manifested resistance comparable to heterozygous controls, suggesting that T-cells were required in the induction of resistance but were not involved in the efferent arm of antibody-dependent elimination. Radiosensitive cells (including eosinophils, basophils, neutrophils, lymphocytes and mast cells) were apparently not essential for the antibody-dependent elimination of lung or post-lung stages since irradiated (700–750 rad.) recipients of VRS manifested comparable degrees of resistance to unirradiated controls in spite of a greater than 85% reduction in total blood leucocyte counts after irradiation. Depletion of 99 % of tissue mast cells by treatment of rats with Compound 48/80 had no significant effect on the attrition of a challenge infection in rats rendered immune by vaccination with irradiated cercariae or by transfer of VRS. However, there was a significant increase in worm recovery in unimmunized and mast cell-depleted or irradiated rats, indicating that mast cells and perhaps other radio-isotope sensitive cells may be involved in innate resistance.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 313 - 326
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bazin, H., Capron, A., Capron, M., Joseph, M., Dessaint, J. P. & Pauwels, R. (1980). Effect of neonatal injection of anti μ antibodies on immunity to schistosomes (Schistosoma mansoni) in the rat. Journal of Immunology 124, 2373.Google Scholar
Capron, A., Dessaint, J-P., Capron, M. & Bazin, H. (1975). Specific IgE antibodies in immune adherence of normal macrophages to Schistosoma mansoni schistosomules. Nature, London 253, 474–5.Google Scholar
Capron, A., Dessaint, J-P., Joseph, M., Rousseaux, R., Capron, M. & Bazin, H. (1977). Interaction between IgE complexes and macrophages in the rat; a new mechanism of macrophage activation. European Journal of Immunology 7, 315–22.CrossRefGoogle Scholar
Capron, A., Dessaint, J-P., Capron, M., Joseph, M. & Pestel, J. (1980). Role of anaphylactic antibodies in immunity to schistosomes. American Journal of Tropical Medicine and Hygiene 29, 849–57.Google Scholar
Capron, A., Dessaint, J-P., Capron, M., Joseph, M. & Torpier, G. (1982). Effector mechanisms of immunity to schistosomes and their regulation. Immunological Reviews 61, 41–66.Google Scholar
Capron, M., Capron, A., Torpier, G., Bazin, H., Bout, D. & Joseph, M. (1978 a). Eosinophil-dependent cytotoxicity in rat schistosomiasis. Involvement of IgG2 antibody and role of mast cells. European Journal of Immunology 8, 127–33.CrossRefGoogle ScholarPubMed
Capron, M., Nogueira-Queiroz, J. A., Papin, J. P. & Capron, A. (1984). Interactions between eosinophils and antibodies: in vivo protective role against rat schistosomiasis. Cellular Immunology 83, 60–72.CrossRefGoogle ScholarPubMed
Capron, M., Rousseaux, J., Mazingue, C., Bazin, H. & Capron, A. (1978 b). Rat mast cell-eosinophil interaction in antibody dependent eosinophil cytotoxity to Schistosoma mansoni schistosom ula. Journal of Immunology 121, 2518–25.Google Scholar
Catto, B. A., Lewis, F. A. & Ottesen, E. A. (1980). Cercariae-induced histamine release: A factor in the pathogenesis of schistosome dermatitis? American Journal of Tropical Medicine and Hygiene 29, 886–9.Google Scholar
Cioli, D., Malorni, W., de Martinino, C. & Dennert, G. (1980). A study of Schistosoma mansoni reinfection in thymectomised rats. Cellular Immunology 53, 246–56.Google Scholar
Clegg, J. A. & Smithers, S. R. (1968). Death of schistosome cercariae during penetration of the skin. II. Penetration of mamalian skin by Schistosoma mansoni. Parasitology 58, 111–28.CrossRefGoogle Scholar
Ford, M. J. (1985). Studies on the mechanisms of induction and expression of resistance against Schistosoma mansoni. Ph.D. thesis, University of London.Google Scholar
Ford, M. J., Bickle, Q. D., Taylor, M. G. & Andrews, B. J. (1984 a). Passive transfer of resistance and the site of attrition of the challenge infection in rats vaccinated with highly irradiated cercariae of Schistosoma mansoni. Parasitology 89, 416–82.Google Scholar
Ford, M. J., Bickle, Q. D. & Taylor, M. G. (1984 b). Immunization of rats against Schistosoma mansoni using irradiated cercariae, lung schistosomula and liver stage worms. Parasitology 89, 327–34.CrossRefGoogle ScholarPubMed
Ghandour, A. M. & Webbe, G. (1973). A study of the death of Schistosoma mansoni during penetration of mammalian host skin: influence of the age of the cercariae and of the host. International Journal for Parasitology 3, 789–94.Google Scholar
GÓes, A. M. & Ramalho-Pinto, F. J. (1980). Avaliação da participação do complemento na Immunidade ao Schistosoma mansoni em ratos. Ciência e cultura 32, 613.Google Scholar
Incani, R. N. & McLaren, D. J. (1981). Neutrophil-mediated cytotoxicity to schistosomula of Schistosoma mansoni in vitro: Studies on the kinetics of complement and/or antibody-dependent adherence and killing. Parasite Immunology 3, 107–26.Google Scholar
Joseph, M. (1982). Effector functions of phagocytic cells against helminths. Clinics in Immunology and Allergy 2, 541–96.Google Scholar
Joseph, M., Auriault, C., Capron, A., Vorng, H., Viens, P. (1983). A new function for platelets: IgE-dependent killing of schistosomes. Nature, London 303, 810–12.Google Scholar
Lewert, R. M. & Mandlowitz, S. (1963). Innate immunity to Schistosoma mansoni relative to state of connective tissue. Annals of the New York Academy of Science 113, 54–62.Google Scholar
Mangold, B. L. & Dean, D. A. (1983). Autoradiographic analysis of Schistosoma mansoni migration from the skin to the lungs in naive mice. Evidence that most of the attrition occurs after the skin phase. American Journal of Tropical Medicine and Hygiene 32, 785–9.CrossRefGoogle Scholar
Mangold, B. L. & Knopf, P. M. (1981). Host protective humoral immune responses to Schistosoma mansoni infections in the rat. Kinetics of hyperimmune serum-dependent sensitivity and elimination of schistosomes in a passive transfer system. Parasitology 83, 559–74.CrossRefGoogle Scholar
Mastin, A. J., Bickle, Q. D. & Wilson, R. A. (1983). Schistosoma mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse. Parasitology 87, 87–102.Google Scholar
Phillips, S. M., Bentley, A. G., Linette, G., Doughty, B. & Capron, M. (1983). The immunological response of congenitally athymic rats to Schistosoma mansoni infection. I. In vivo studies of resistance. Journal of Immunology 131, 1466–74.Google Scholar
Phillips, S. M., Reid, W. A., Bruce, J. I., Hedlund, K., Colvin, R. C., Campbell, R., Diggs, C. L. & Sadun, E. H. (1975). The cellular and humoral response to Schistosoma mansoni infection in the rat. I. Mechanisms during initial exposure. Cellular Immunology 19, 99–116.Google Scholar
Phillips, S. M., Reid, W. A. & Sadun, E. H. (1977). The cellular and humoral immune response to Schistosoma mansoni infection in inbred rats. II. Mechanisms during reexposure. Cellular Immunology 28, 75–89.Google Scholar
Riley, J. F. (1959). The effects of a specific histamine-liberator compound 48/80, on the mast cells of the rat. In The Mast Cells, pp. 93–108. Edinburgh and London: E. S. Livingston Ltd.CrossRefGoogle Scholar
Sher, A., Hieny, S., James, S. L. & Asofsky, R. (1982). Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae (II). Analysis of immunity in hosts deficient in T lymphocytes, B lymphocytes, or complement. Journal of Immunology 128, 1880–4.CrossRefGoogle ScholarPubMed
Smithers, S. R. (1976). Immunity to trematode infections. In Immunology of Parasitic Infections, (ed. Cohen, S. and Sadun, E. H.), pp. 296–332. Oxford: Blackwell Scientific Publications.Google Scholar
Smithers, S. R. & Terry, R. J. (1969). The immunology of schistosomiasis. In Advances in Parasitology, vol. 7, (ed. Dawes, B.), pp. 41–93. London and New York: Academic Press.Google Scholar