Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-28T05:33:03.495Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunity to coccidiosis: protective effects of transferred serum and cells investigated in chick embryos infected with Eimeria tenella

Published online by Cambridge University Press:  06 April 2009

M. Elaine Rose  and
P. L. Long
M. Elaine Rose
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon
P. L. Long
Affiliation:
Houghton Poultry Research Station, Houghton, Huntingdon
Get access Rights & Permissions [Opens in a new window]

Summary

The chick embryo proved to be an eminently suitable medium for immunological studies on E. tenella infection, some of the difficulties encountered in the normal host, the hatched chicken, being circumvented.

Adoptive transfer of immunity to E. tenella infections in the chick embryo was obtained with antibodies and with cells from caecal tonsils. Protective factors (antibodies) were demonstrated in the serum of fowls which had been infected with E. tenella and in their progeny. Suspensions of caecal tosnil cells inoculated on to the CAM of developing chick embryos reduced the extent of subsequent infections with E. tenella and this was particularly marked when the cell donors had been immunized with E. tenella.

Serum globulin protected only when the donors were becoming immune, i.e. after the third inoculum of E. tenella oocysts. The experiment was not continued to the point at which the serum donors were ‘solidly immune’, when previous attempts to protect passively with serum have failed.

Maternally transmitted protection was demonstrated in embryos only at the time at which peak protection was afforded by serum globulin, but this is probably an unsatisfactory method of testing since antibodies are not present in allantoic fluid until late in incubation, when coccidial infections are already well established.Some reduction in the oocyst output of the progeny of immunized hens was found when these were challenged at 4 days of age.

The protection given by the inoculation of suspensions of cells from the caecal tonsils may have resulted from the production of antibodies by these cells or from their interaction with the parasite in some form of cell-mediated immune mechanism.

We are grateful to Mrs Patricia Hesketh, Mr Martin Shirley and Mr Ron Waters for technical assistance and to Mr Peter Townsend for care of the animals.

Type
Research Article
Information
Parasitology , Volume 63 , Issue 2 , October 1971 , pp. 299 - 313
Copyright
Copyright © Cambridge University Press 1971

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bovarnick, M. R., Miller, J. C. & Synder, J. C. (1950). The influence of certain salts, sugars and proteins on the stability of Rickettsiae. Journal of Bacteriology 59, 509–22.CrossRefGoogle ScholarPubMed
Boyer, G. S. (1960). Chorioallantoic membrane lesions produced by inoculation of adult fowl leucocytes. Nature, London 185, 327–8.CrossRefGoogle ScholarPubMed
Janković, B. D. & Mitrović, K. (1967). Antibody-producing cells in the chicken as observed by fluorescent antibody technique. Folia biologica (Praha) 13, 406–10.Google ScholarPubMed
Kramer, T. C. & Cho, H. C. (1970). Transfer of immunoglobulins and antibodies in the hen's egg. Immunology 19, 157–67.Google Scholar
Lind, P. E. & Ernyei, S. (1963). Adoptive transfer of antibody production in the chick embryo. 1. The inoculation of stimulated spleen cells from adult fowls on to the chorioallantoic membrane of outbred and inbred embryos. The Australian Journal of Experimental Biology and Medical Science 41, 661–74.CrossRefGoogle Scholar
Long, P. L. (1966). The growth of some species of Eimeria in avian embryos. Parasitology 56, 575–81.CrossRefGoogle Scholar
Long, P. L. (1970). Eimeria tenella: chemotherapeutic studies in chick embryos with a description of a new method (chorioallantoic membrane foci counts) for evaluating infections. Zeitschrift für Parasitenkunde 33, 329–38.CrossRefGoogle Scholar
Long, P. L. (1970 a). Some factors affecting the severity of infection with Eimeria tenella in chicken embryos. Parasitology 60, 435–47.CrossRefGoogle ScholarPubMed
Long, P. L. (1970 b). Studies on the viability of sporozoites of Eimeria tenella. Zeitschrift für Parasitenkunde 35, 1–6.CrossRefGoogle ScholarPubMed
Long, P. L., Rose, M. E. & Pierce, A. E. (1963). Effects of fowl sera on some stages in the life cycle of Eimeria tenella. Experimental Parasitology 14, 210–17.CrossRefGoogle ScholarPubMed
Long, P. L. & Rowell, J. G. (1958). Counting oocysts of chicken coccidia. Laboratory Practice 7, 515–18, 534.Google Scholar
Orlans, E. & Rose, M. E. (1970). Antibody formation by transferred cells in inbred fowls. Immunology 18, 473–82.Google ScholarPubMed
Patterson, R., Youngner, J. S., Weigle, W. O. & Dixon, F. J. (1962). Antibody production and transfer to egg yolk in chickens. Journal of Immunology 89, 272–8.CrossRefGoogle ScholarPubMed
Pierce, A. E., Long, P. L. & Horton-Smith, C. (1963). Attempts to induce a passive immunity to Eimeria tenella in young fowls (Gallus domesticus). Immunology 6, 37–47.Google ScholarPubMed
Rose, M. E. (1971). Immunity to coccidiosis: protective effect of transferred serum in Eimeria maxima infections. Parasitology 62, 11–25.CrossRefGoogle ScholarPubMed
Simonsen, M. (1957). The impact on the developing embryo and newborn animal of adult homologous cells. Ada pathologica et microbiologica scandinavica 40, 480–500.CrossRefGoogle ScholarPubMed
Tucker, D. F. & Owen, J. J. T. (1969). Growth of tumour cells in diffusion chambers on the chick chorioallantois. European Journal of Cancer 5, 591–6.CrossRefGoogle ScholarPubMed