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IgG response of rats and humans to the released products of schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

Claudie Verwaerde ,
C. Auriault ,
Martine Damonneville ,
J.-M. Grzych ,
R. Pierce  and
A. Capron
Claudie Verwaerde
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
C. Auriault
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
Martine Damonneville
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
J.-M. Grzych
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
R. Pierce
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
A. Capron
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, 15 rue Camille Guérin, 59019 Lille cedex, France
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Extract

The participation of products released from Schistosoma mansoni schistosomula (SRP-A) in the IgG antibody response of infected Brown-Norway rats and infected humans has been studied using immunoprecipitation with various antigenic preparations and in in vitro cytotoxicity assays. A large number of SRP-A molecules with a wide range of molecular weights was recognized by infected rat and human sera. Anti-SRP-A antibodies appeared in rat sera from day 28 after infection. In infected humans, a variable pattern of SRP-A recognition was observed between individuals. IgG antibodies obtained by immunization of rats with SRP-A without addition of adjuvants reacted with 3 major schistosomula surface proteins with molecular weights of 38, 32 and 21 kDa. These latter molecules were also revealed strongly by infected rat sera. Moreover, these antibodies were able to kill schistosomula in vitro in the presence of complement or eosinophils.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 3 , June 1985 , pp. 509 - 518
Copyright
Copyright © Cambridge University Press 1985

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References

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