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Identification of some heat-induced genes of Trichinella spiralis

Published online by Cambridge University Press:  28 November 2001

C. H. MAK ,
K. W. SUN  and
R. C. KO
C. H. MAK
Affiliation:
Department of Zoology, The University of Hong Kong, Pokfulam, Hong Kong, China
K. W. SUN
Affiliation:
Department of Zoology, The University of Hong Kong, Pokfulam, Hong Kong, China
R. C. KO
Affiliation:
Department of Zoology, The University of Hong Kong, Pokfulam, Hong Kong, China
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Abstract

Three heat-induced genes of the infective-stage larvae of Trichinella spiralis were successfully identified by the suppression subtractive hybridization (SSH) technique. As indicated by reverse Northern blotting, 19 of 25 clones were scored as differentially transcribed in the heat-shocked infective-stage larvae. The sequencing data showed the presence of 12 different genes. Three were homologous to histone H3, histone H2B and translationally controlled tumour protein (TCTP). A 0.6 kb cDNA of histone H3 was generated by the RACE method and sequenced. It contained an open reading frame of 136 amino acids that demonstrated 94% identity with genes from Drosophila hydei. Semi-quantitative RT-PCR indicated that after heat-shock treatment, the expression levels of histone H3, histone H2B and TCTP increased 4.8, 27 and 5.7-fold, respectively. Northern analysis confirmed the upregulation of histone H3, histone H2B and TCTP transcripts. The upregulation of these genes during stress conditions has not been reported in parasitic organisms. The stress proteins may play an active role to sustain the parasite after exposure to hostile host factors.

Keywords

Trichinella spiralisheat-induced geneshistonetranslationally controlled tumour proteinsuppression subtractive hybridization
Type
Research Article
Information
Parasitology , Volume 123 , Issue 3 , March 2001 , pp. 293 - 300
Copyright
© 2002 Cambridge University Press

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