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Identification of non-cross-reacting antigens of Onchocerca volvulus with lymphatic filariasis serum pools

Published online by Cambridge University Press:  06 April 2009

E. Lobos  and
N. Weiss
E. Lobos
Affiliation:
Laboratory of Immunoparasitology, Swiss Tropical Institute, Socinstrasse 57, 4051 Basel, Switzerland
N. Weiss
Affiliation:
Laboratory of Immunoparasitology, Swiss Tropical Institute, Socinstrasse 57, 4051 Basel, Switzerland
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Summary

Onchocerca volvulus proteins labelled with 125I were immunoprecipitated with onchocerciasis and lymphatic filariasis human serum pools in order to differentiate between cross-reacting and non-cross-reacting antigens. Analysis of the immunoprecipitates by two-dimensional gel electrophoresis revealed that all high molecular weight (Mr) O. volvulus antigens cross-reacted with the lymphatic filariasis serum pools. We observed, however, that at least 8 0. volvulus antigens were specifically immunoprecipitated only by the onchocerciasis serum pools, with Mr ranging from 20000 up to 43000 Daltons. These results suggest that the lower MrO. volvulus antigens are more species specific than the other antigens. The significance of these findings for the immunodiagnosis of onchocerciasis is discussed.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 2 , October 1986 , pp. 389 - 399
Copyright
Copyright © Cambridge University Press 1986

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