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Identification of a fibronectin-like molecule on Eimeria tenella

Published online by Cambridge University Press:  06 April 2009

F. Lopez-Bernad ,
E. Del Cacho ,
M. Gallego ,
J. Quilez  and
C. Sanchez-Acedo
F. Lopez-Bernad
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013-Zaragoza, Spain
E. Del Cacho*
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013-Zaragoza, Spain
M. Gallego
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013-Zaragoza, Spain
J. Quilez
Affiliation:
Department of Pathology and Animal Production, Faculty of Veterinary Sciences, 08193-Bellaterra, Barcelona, Spain
C. Sanchez-Acedo
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013-Zaragoza, Spain
*
* Corresponding author: Parasitologia y Enfermedades Parasitarias, Facultad de Veterinaria, Miguel Servet 177, 50013-Zaragoza, Spain. +34 76 761556. Fax: + 34 76 761612.
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Summary

The attachment of Eimeria tenella to its target cells as an obligatory intracellular pathogen is essential for the development of disease. Previous reports have established that other intracellular protozoa parasites have either fibronectin, an adhesion protein, or fibronectin receptors, both of which are involved in the interaction with the host cells. In this current research, studies have been undertaken to visualize a surface component that may be involved in the attachment of E. tenella to host cells. For this purpose, monoclonal antibodies, both anti-chicken and anti-human fibronectin, and also anti-chicken integrin were used. Our results show a fibronectin-like molecule with an apparent molecular weight of 110 kDa in mature schizonts and microgametes. Staining with serum directed against chicken integrin revealed immunoreactivity within mature schizonts. Both the fibronectin-like molecule and the integrin may play an important role in the parasite stage-cell interaction and the promotion of parasite uptake.

Keywords

fibronectinintegrinadhesion moleculesEimeria tenellachicken
Type
Research Article
Information
Parasitology , Volume 113 , Issue 6 , December 1996 , pp. 505 - 510
Copyright
Copyright © Cambridge University Press 1996

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