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Hybridization experiments indicate incomplete reproductive isolating mechanism between Fasciola hepatica and Fasciola gigantica

Published online by Cambridge University Press:  18 July 2011

T. ITAGAKI ,
M. ICHINOMIYA ,
K. FUKUDA ,
S. FUSYUKU  and
C. CARMONA
T. ITAGAKI*
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan
M. ICHINOMIYA
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan
K. FUKUDA
Affiliation:
Center for Laboratory Animal Science, National Defense Medical College, Tokorozawa 359-8513, Japan
S. FUSYUKU
Affiliation:
Center for Laboratory Animal Science, National Defense Medical College, Tokorozawa 359-8513, Japan
C. CARMONA
Affiliation:
Laboratory of Parasitology, Institute of Hygiene, Montevideo, Uruguay
*
*Corresponding author: Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan. Tel: and Fax: +81 19 621 6219. E-mail: [email protected]
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Summary

Experiments on hybridization between Fasciola hepatica and Fasciola gigantica were carried out to clarify whether a reproductive isolating mechanism appears between the two Fasciola species. Molecular evidence for hybridization was based on the DNA sequence of the internal transcribed spacer 1 (ITS1) region in nuclear ribosomal DNA, which differs between the species. The results suggested that there were not pre-mating but post-mating isolating mechanisms between the two species. However, viable adults of the hybrids F1 and F2 were produced from both parental F. hepatica and F. gigantica. The hybrids inherited phenotypic characteristics such as ratio of body length and width and infectivity to rats from parental Fasciola hepatica and F. gigantica. These findings suggest that reproductive isolation is incomplete between Fasciola hepatica and F. gigantica. Adults of the hybrids F1 and F2 were completely different in mode of reproduction from aspermic Fasciola forms that occur in Asia and seem to be offspring originated from hybridization between F. hepatica and F. gigantica and to reproduce parthenogenetically.

Keywords

Fasciola hepaticaFasciola giganticahybridizationhybridsF1F2reproductive isolation
Type
Research Article
Information
Parasitology , Volume 138 , Issue 10 , September 2011 , pp. 1278 - 1284
Copyright
Copyright © Cambridge University Press 2011

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