Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-23T16:38:06.084Z Has data issue: false hasContentIssue false
  • English
  • Français

Histochemical observations on the occurrence of carbohydrates, lipids and enzymes in the daughter sporocyst of Cercaria bucephalopsis haimaena Lacaze-Duthiers, 1854 (Digenea: Bucephalidae).

Published online by Cambridge University Press:  06 April 2009

B. L. James  and
E. A. Bowers
B. L. James
Affiliation:
Department of Zoology, University College, Swansea
E. A. Bowers
Affiliation:
Department of Zoology, University College, Swansea
Get access Rights & Permissions [Opens in a new window]

Extract

The distribution of carbohydrates, lipids and enzymes in the daughter sporocyst and contained cercariae of Cercaria bucephalopsis haimaena Lacaze-Duthiers, 1854, is mapped and used as an indication of metabolism. It is concluded that host glucose, glycogen, fatty acids and neutral lipids are absorbed and pass through the syncytial tegument into the cellular sub-tegument. They are then either metabolized or passed into the body cavity and contained cercariae. Glucose seems to be either converted into glycogen and glycoproteins or metabolized anaerobically. The end products of anaerobic respiration are fatty acids and neutral lipids. These pass into the excretory system, which flushes them into the body cavity of the daughter sporocyst, from where they enter the body of the developing cercariae. In the cercariae they are deposited, together with host lipids, possibly as less toxic higher fatty acids or other stored lipids. The stored glycogen and lipids in the cercariae are probably utilized aerobically during the free-living existence. The transport of nutrients and excretory products across the cell membranes may be aided by alkaline phosphatase and esterase activity, as is the metabolism of carbohydrates and lipids. Acid mucopolysaccharide precursors appear to be synthesized in the dense cytoplasm of the mid-tegument of the daughter sporocyst and secreted into the host's visceral haemocoel. Acid phosphatase and esterase found in this region may be involved in the synthesis and also in the breakdown of complex incoming nutrients.

We are grateful to Professor E. W. Knight-Jones for laboratory facilities and to the Science Research Council for a grant to one of us (E. A. B.) which made the work possible.

Type
Research Article
Information
Parasitology , Volume 57 , Issue 1 , February 1967 , pp. 79 - 86
Copyright
Copyright © Cambridge University Press 1967

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Axmann, M. C. (1947). Morphological studies on glycogen deposition in schistosomes and other flukes. J. Morph. 80, 321–34.CrossRefGoogle ScholarPubMed
Bowers, E. A. (1965). Studies on some parasites of sea birds and molluscs. Ph.D. Thesis, University College, Swansea.Google Scholar
Brand, T. von, McMahon, P., Gibbs, E. & Higgins, H. (1964). Aerobic and anaerobic metabolism of larval and adult Taenia taeniaeformis. II. Hexose leakage and absorption; tissue glucose and polysaccharides. Expl Parasit. 15, 410–29.CrossRefGoogle Scholar
Cheng, T. C. (1963 a). The effect of Echinoparyphium larvae on the structure of and glycogen deposition in the hepatopancreas of Helisoma trivolvis and glycogenesis in the parasite larva. Malacologia 1, 291–303.Google Scholar
Cheng, T. C. (1963 b). Histological and histochemical studies on the effects of parasitism of Musculium partumeium (Say) by the larvae of Gorgodera amplicava Looss. Proc. helminth. Soc. Wash. 30, 101–7.Google Scholar
Cheng, T. C. (1963 c). Biochemical requirements of larval trematodes. Ann. N.Y. Acad. Sci. 113, 289–321.CrossRefGoogle ScholarPubMed
Cheng, T. C. (1964). Studies on phosphatase systems in hepatopancreatic cells of the molluscan host of Echinoparyphium sp. and in the rediae and cercariae of this trematode. Parasitology 54, 73–79.CrossRefGoogle ScholarPubMed
Cheng, T. C. & James, H. A. (1960). The histopathology of Crepidostomum sp. infection in the second intermediate host, Sphaerium striatinum. Proc. helminth. Soc. Wash. 27, 67–68.Google Scholar
Cheng, T. C. & Snyder, R. W. (1962 a). Studies on host-parasite relationships between larval trematodes and their hosts. I. A review. II. The utilization of the host's glycogen by the intramolluscan larvae of Glypthelmins pennsylvaniensia Cheng, and related phenomena. Trans. Am. microsc. Soc. 81, 209–28.CrossRefGoogle Scholar
Cheng, T. C. & Snyder, R. W. (1962 b). Studies on host–parasite relationships between larval trematodes and their hosts. III. Certain aspects of lipid metabolism in Helisoma trivolvis (Say) infected with the larvae of Glypthelmins pennsylvaniensis Cheng and related phenomena. Trans. Am. microsc. Soc. 81, 327–31.CrossRefGoogle Scholar
Cheng, T. C. & Snyder, R. W. (1963). Studies on host–parasite relationships between larval trematodes and their hosts. IV. A histochemical determination of glucose and its role in the metabolism of molluscan host and parasite. Trans. Am. microsc. Soc. 82, 343–46.CrossRefGoogle Scholar
Coil, W. H. (1958). Alkaline phosphatase in the trematode excretory system. Proc. helminth. Soc. Wash. 25, 137–8.Google Scholar
Danielli, J. F. (1952). Structural factors in cell permeability and secretion. Symp. Soc. Exp. Biol. No. 6. Structural Aspects of Cell Physiology. Cambridge University Press.Google Scholar
Erasmus, D. A. (1957). Studies on phosphatase systems of cestodes. 1. The enzymes present in Taenia pisiformis (cysticercus and adult). Parasitology 47, 70–80.CrossRefGoogle Scholar
Friedl, F. E. (1961 a). Studies on larval Fascioloides magna. 1. Observations on the survival of rediae in vitro. J. Parasit. 47, 71–5.CrossRefGoogle Scholar
Friedl, F. E. (1961 b). Studies on larval Fascioloides magna. II. In vitro survival of axenie rediae in amino acids and sugars. J. Parasit. 47, 244–7.CrossRefGoogle Scholar
Ginetsinskaia, T. A. (1960). Glycogen in the body of cercariae and the dependence of its distribution upon the peculiarities of their biology. (In Russian.) Dokl. Akad. Nauk SSSR 135, 1012–15.Google Scholar
Ginetsinskaia, T. A. (1961). The dynamics of the storage of fat in the course of the life cycle of trematodes. (In Russian.) Dokl. Akad. Nauk. SSSR 139, 1016–19.Google Scholar
Ginetsinskaia, T. A. & Dobrovolsky, A. A. (1962). Glycogen and fat in the various phases of the life cycle of trematodes. (In Russian.) Vest. leningr. gos. Univ. 9, Biol. ser no. 2, 67–81.Google Scholar
Ginetsinskaia, T. A. & Dobrovolsky, A. A. (1963). Glycogen and fat in the various phases of the life cycle of trematodes. Part II. Biological significance of glycogen and fat. (In Russian.) Vest. leningr. gos. Univ. 18, Biol. ser. no. 1, 23–33.Google Scholar
Ivanov, I. I. (1950). On the biochemistry of the muscular apparatus of helminths. (In Russian.) Trudy¯ Uses. Inst. Gel'mint. 4, 51–7.Google Scholar
James, B. L. (1965). The effects of parasitism by larval Digenea on the digestive gland of the intertidal prosobranch, Littorina saxatilis (Olivi) subsp. tenebrosa (Montagu). Parasitology 55, 93–115.CrossRefGoogle Scholar
James, B. L. & Bowers, E. A. (1967 a). The effects of parasitism by the daughter sporocyst of Cercaria bucephalopsis haimaena Lacaze-Duthiers, 1854, on the digestive tubules of the cockle, Cardium edule L. Parasitology 57, 67–77.CrossRefGoogle Scholar
James, B. L. & Bowers, E. A. (1967 b). The germ cell cycle and method of reproduction in the daughter sporocysts of Cercaria bucephalopsis haimaena Lacaze-Duthiers, 1854 (Bucephalidae) and Cercaria dichotoma Lebour, 1911 (Gymnophallidae), together with a review of the interpretative theories advanced to explain the life cycle of the Digenea. Parasitology (in preparation).Google Scholar
James, B. L., Bowers, E. A. & Richards, J. G. (1966). The ultrastructure of the daughter sporocyst of Cercaria bucephalopsis haimaena Lacaze-Duthiers, 1854 (Digenea: Bucephalidae) from the edible cockle, Cardium edule L. Parasitology 56, 753–62.CrossRefGoogle ScholarPubMed
Lee, D. L., Rothman, A. H. & Senturia, J. B. (1963). Esterases in Hymenolepis and in Hydatigera. Expl Parasit. 14, 285–95.CrossRefGoogle ScholarPubMed
Lee, D. L. & Tatchell, R. J. (1964). Studies on the tapeworm Anoplocephala perfoliata (Goeze, 1782). Parasitology 54, 467–79.CrossRefGoogle ScholarPubMed
Lutta, A. S. (1939). The dynamics of the glycogen and fat in parasitic worms. (In Russian.) Uchen. Zap. leningr. gos. Univ. Ser. 11, 129–71.Google Scholar
Magalhaes, N. B. & de Almeida, A. M. (1956). Influencia des dessecacão sobre oteor de glicogenio dos tecidos em Australorbis glabratus. Publções avuls. Inst. Aggeu Magalhães. 5, 1–5.Google Scholar
Pearse, A. G. E. (1961). Histochemistry, Theoretical and Applied, 2nd ed.998 pp. London: J. and A. Churchill Ltd.Google Scholar
Phifer, K. (1960). Permeation and membrane transport in animal parasites: on the mechanism of glucose uptake by Hymenolepis diminuta. J. Parasit. 46, 145–53.CrossRefGoogle ScholarPubMed
Schware, C. W. (1959). Host-parasite relationships in echinococcosis. 1. Observations on the permeability of the hydatid cyst wall. Am. J. trop. Med. Hyg. 8, 20–28.CrossRefGoogle Scholar
Schwabe, C. W., Koussa, M. & Acra, A. N. (1961). Host-parasite relationships in echinococcosis. II. Acetylcholinesterase and permeability regulation in the hydatid cyst wall. Comp. Biochem. Phys. 2, 161–72.CrossRefGoogle Scholar
Simoeo, B. F. & Coelho, M. V. (1955). Comportamento formos larvãrias de Schistosoma mansoni em Australorbis glabratus (Mollusca: Planorbidae), subjeito a'estivacão. Publções avuls. Inst. Aggeu Magalhães 4, 51–60.Google Scholar
Vernberg, N. B. (1962). Substrates utilized in vitro by different stages in the life cycle of the trematode, Himasthla quissetensis (Echinostomatidae). J. Parasit. 48, Suppl. 21. (Abstr.)Google Scholar
Vernberg, N. B. & Hunter, W. S. (1963). Utilization of certain substrates by larval and adult stages of Himasthla quissetensis. Expl Parasit. 14, 311–15.CrossRefGoogle ScholarPubMed