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Histochemical demonstration of acetylcholinesterase in sporocysts of Schistosoma mansoni (Trematoda)

Published online by Cambridge University Press:  06 April 2009

Joseph J. Diconza  and
Paul F. Basch
Joseph J. Diconza
Affiliation:
Department of Family, Community and Preventive Medicine, Stanford University, Stanford, California94305
Paul F. Basch
Affiliation:
Department of Family, Community and Preventive Medicine, Stanford University, Stanford, California94305
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Extract

The distribution of acetylcholinesterase in mother and daughter sporocysts of Schistosoma mansoni was studied histochemically. In young mother sporocysts derived from miracidia cultured in vitro the miracidial neural mass and flame cells were shown to persist. The nerve trunks and commissures, as well as papillae, are apparently lost in the transformation process. In young daughter sporocysts freshly dissected from mother sporocysts there was little enzyme activity except for a sparse distribution in the tegument. After cultivation, intense enzyme activity was associated with developing cercarial embryos. A similar distribution of activity was observed in older daughter sporocysts obtained from the digestive gland of snails. No evidence of flame cells, neural mass, or commissures was detected in daughter sporocysts by the methods employed.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 2 , October 1975 , pp. 305 - 310
Copyright
Copyright © Cambridge University Press 1975

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