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High resolution DNA fingerprinting by AFLP to study the genetic variation among Oesophagostomum bifurcum (Nematoda) from human and non-human primates from Ghana

Published online by Cambridge University Press:  27 October 2004

J. M. DE GRUIJTER ,
R. B. GASSER ,
A. M. POLDERMAN ,
V. ASIGRI  and
LENIE DIJKSHOORN
J. M. DE GRUIJTER
Affiliation:
Department of Parasitology, Leiden University Medical Center, University of Leiden, PO Box 9600, 2300 RC Leiden, The Netherlands
R. B. GASSER
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia
A. M. POLDERMAN
Affiliation:
Department of Parasitology, Leiden University Medical Center, University of Leiden, PO Box 9600, 2300 RC Leiden, The Netherlands
V. ASIGRI
Affiliation:
Parasitic Diseases Research Laboratory, PO Box 967, Tamale, Ghana
LENIE DIJKSHOORN
Affiliation:
Department of Infectious Diseases, Leiden University Medical Center, University of Leiden, PO Box 9600, 2300 RC Leiden, The Netherlands
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Abstract

An AFLP approach was established to investigate genetic diversity within Oesophagostomum bifurcum (order Strongylida) from human and non-human primates. Evaluation of different combinations of restriction enzymes (n=8) and primers (n=29) demonstrated that the use of HindIII/BglII digested templates and primers with the selective nucleotides +AG/+AC, respectively, was the most effective for the analysis of O. bifurcum DNA. A total of 63 O. bifurcum adults from human, Patas monkey, Mona monkey and Olive baboon hosts from different geographical regions in Ghana were subjected to analysis using this method. Cluster analysis revealed 4 genetically distinct groups, namely O. bifurcum from the Patas monkey (I), from the Mona monkey (II), from humans (III) and from the Olive baboon (IV). These findings were concordant with those achieved previously using RAPD analysis and supports population genetic substructuring within O. bifurcum according to host species. The results demonstrated the effectiveness of the present AFLP method for establishing genetic variation within O. bifurcum, and indicates its applicability to other parasitic nematodes of human and/or veterinary health importance.

Keywords

Oesophagostomum bifurcumpopulation genetic variationAFLP
Type
Research Article
Information
Parasitology , Volume 130 , Issue 2 , February 2005 , pp. 229 - 237
Copyright
© 2005 Cambridge University Press

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