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The gull-tapeworm, Diphyllobothrium dendriticum and neuropeptide F: an immunocytochemical study

Published online by Cambridge University Press:  06 April 2009

M. K. S. Gustafsson ,
D. W. Halton ,
A. G. Maule ,
M. Reuter  and
C. Shaw
M. K. S. Gustafsson
Affiliation:
Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520 Åbo, Finland
D. W. Halton
Affiliation:
Comparative Neuroendocrinology Research Group, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
A. G. Maule
Affiliation:
Comparative Neuroendocrinology Research Group, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
M. Reuter
Affiliation:
Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520 Åbo, Finland
C. Shaw
Affiliation:
Comparative Neuroendocrinology Research Group, The Queen's University of Belfast, Belfast BT7 1NN, Northern Ireland
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Extract

Neuropeptide F (Moniezia expansa) immunoreactivity (NPF-IR) has been detected in the nervous system of plerocercoid and adult stages of the gull-tapeworm Diphyllobothrium dendriticum, using immunocytochemical methodology. The application of the antiserum for this authentic flatworm neuropeptide to whole-mounts and frozen sections of the worm has resulted in new information about its neuroanatomy. Thus, at regular intervals, transverse nerves extend from the main nerve cords laterally, joining the longitudinal lateral minor cords in the cortical parenchyma. In the adult worm, the transverse nerves are located at the posterior border of each proglottis. The medullary parenchyma lacks NPF-IR. The NPF-immunoreactive cell bodies are bi- to multipolar and preferentially located in the peripheral nervous system, in close association with the holdfast musculature of the scolex and the extensive body musculature. NPF-IR was observed in the innervation to the muscular ducts of the reproductive system. The pattern of NPF-IR was compared with that recorded for RFamide- and 5-HT-IR and double-immunostaining has revealed separate populations of serotoninergic and peptidergic neurones.

Keywords

Diphyllobothrium dendriticumnervous systemneuropeptidesneuropeptide F
Type
Research Article
Information
Parasitology , Volume 109 , Issue 5 , December 1994 , pp. 599 - 609
Copyright
Copyright © Cambridge University Press 1994

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