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A glycoprotein specific to the amphids of Meloidogyne species

Published online by Cambridge University Press:  06 April 2009

G. R. Stewart ,
R. N. Perry ,
J. Alexander  and
D. J. Wright
G. R. Stewart
Affiliation:
Department of Biology, Imperial College, Silwood Park, Ascot, BerksSL5 7PY Entomology and Nematology Department, AFRC IACR, Rothamsted Experimental Station, Harpenden Herts AL5 2JQ
R. N. Perry
Affiliation:
Entomology and Nematology Department, AFRC IACR, Rothamsted Experimental Station, Harpenden Herts AL5 2JQ
J. Alexander
Affiliation:
Department of Immunology, The Todd Centre, University of Strathclyde, Glasgow G4 0NR
D. J. Wright*
Affiliation:
Department of Biology, Imperial College, Silwood Park, Ascot, BerksSL5 7PY
*
*Reprint request to Dr D. J. Wright, Department of Biology, Imperial College, Silwood Park, Ascot, Berks SL5 7PY.
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Summary

Indirect immunofluorescence studies using a rabbit polyclonal antiserum have been used to localize the presence of a 32 kDa glycoprotein in the region of the amphids of 2nd-stage juveniles of the root-knot nematode, Meloidogyne incognita. Similar immunoreactivity was also demonstrated in 5 other Meloidogyne species but was not found in representatives of 8 other nematode genera including the closely related cyst nematodes (Globodera and Heterodera). Immunoelectron microscopical studies have shown that the immunoreactivity in M. incognita is associated with the secretory material filling the amphidial channel and probably with the sheath cell.

Keywords

plant-parasitic nematodesamphidssense organspolyclonal antibodyimmunocytochemistry.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 4 , May 1993 , pp. 405 - 412
Copyright
Copyright © Cambridge University Press 1993

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