Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-27T02:56:39.886Z Has data issue: false hasContentIssue false
  • English
  • Français

Glucose phosphate isomerase isozymes as genetic markers for lines of Eimeria tenella

Published online by Cambridge University Press:  06 April 2009

T. Nakamura ,
T. Konishi ,
H. Kawaguchi  and
Y. Hayashi
T. Nakamura
Affiliation:
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi and Co., Ltd, Koka-cho, Shiga 520–34, Japan
T. Konishi
Affiliation:
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi and Co., Ltd, Koka-cho, Shiga 520–34, Japan
H. Kawaguchi
Affiliation:
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi and Co., Ltd, Koka-cho, Shiga 520–34, Japan
Y. Hayashi
Affiliation:
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi and Co., Ltd, Koka-cho, Shiga 520–34, Japan
Get access Rights & Permissions [Opens in a new window]

Summary

Two strains of Eimeria tenella with different decoquinate sensitivity and different glucose phosphate isomerase (GPI) isozymes were used in genetic recombination experiments: a line derived from a laboratory strain (NIAH) was decoquinate-resistant (DR) and had the isozyme GPI-9, while a field isolate (Iwate strain) was decoquinate-sensitive (DS) and had GPI-1. Coccidia-free chickens were orally inoculated with mixed oocysts of the two strains and parasites of the F1 generation were recovered. The F1 progeny showed both forms of the isozyme. Next, oocysts of the F1 progeny were passaged through chickens given the decoquinate-containing diet. The F2 progeny also had GPI-1 and GPI-9, indicating crossfertilization between the two strains. Six single oocysts were isolated from F2 progeny; 1 showed both phenotypes of GPI, 1 had GPI-1 and the remaining 4 lines had GPI-9. Analysis of the amount of GPI in recombinant oocysts suggested that the proliferation rate of the DR strain was slower than that of the DS strain. We concluded that GPI isozymes in E. tenella can serve as useful markers in experiments on chicken coccidia.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 2 , April 1988 , pp. 281 - 288
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Canning, E. U. & Anwar, M. (1968). Studies on meiotic division in coccidial and malarial parasites. Journal of Protozoology 15, 290–8.Google Scholar
Chapman, H. D. (1975). Eimeria tenella in chickens: development of resistance to quinolone anticoccidial drugs. Parasitology 71, 41–9.Google Scholar
Chapman, H. D. (1982). The use of enzyme electrophoresis for the identification of the species of Eimeria present in field isolates of coccidia. Parasitology 85, 437–42.Google Scholar
Chapman, H. D. (1986). Eimeria tenella: stability of resistance of halofuginone, decoquinate and arpronocid in the chicken. Research in Veterinary Science 40, 139–40.Google Scholar
Jeffers, T. K. (1974). Genetic transfer of anticoccidial drug resistance in Eimeria tenella. Journal of Parasitology 60, 900–4.CrossRefGoogle ScholarPubMed
Jeffers, T. K. (1976). Genetic recombination of precociousness and anticoccidial drug resistance in Eimeria tenella. Zeitschrift für Parasitenkunde 50, 251–5.Google Scholar
Joyner, L. P. & Norton, C. C. (1975). Transferred drug-resistance in Eimeria maxima. Parasitology 71, 385–92.CrossRefGoogle ScholarPubMed
Joyner, L. P. & Norton, C. C. (1977). Further observations on the genetic transfer of drug resistance in Eimeria maxima. Parasitology 74, 205–13.Google Scholar
Long, P. L., Millard, B. J. & Shirley, M. W. (1977). Strain variation within Eimeria meleagrimitis from the turkey. Parasitology 74, 177–82.Google Scholar
Nakamura, T., Konishi, T. & Kawaguchi, H. (1986). Isozymes of chicken coccidia in Japan. Japanese Journal of Veterinary Science 48, 587–90.Google ScholarPubMed
Rollinson, D., Joyner, L. P. & Norton, C. C. (1979). Eimeria maxima: the use of enzyme markers to detect the genetic transfer of drug resistance between lines. Parasitology 78, 361–7.Google Scholar
Ryley, J. F. (1980). Drug resistance in coccidia. Advances in Veterinary Science and Comparative Medicine 24, 99–120.Google Scholar
Shirley, M. W. (1975). Enzyme variation of Eimeria species of the chickens. Parasitology 71, 369–76.CrossRefGoogle Scholar
Shirley, M. W. (1978). Electrophoretic variation of enzymes: a further marker for genetic studies of the Eimeria. Zeilschrift für Parasitenkunde 57, 83–7.CrossRefGoogle ScholarPubMed
Shirley, M. W. & Rollinson, D. (1979). Coccidia: the recognition and characterization of populations of Eimeria. In Problems in the Identification of Parasites and Their Vectors. Symposia of the British Society for Parasitology, vol. 17 (ed. Taylor, A. E. R. and Muller, R.), pp. 7–30. Oxford: Blackwell.Google Scholar
Sutton, C. A., Shirley, M. W. & McDonald, V. (1986). Genetic recombination of markers for precocious development. Arprinocid resistance, and isoenzymes of glucose phosphate isomerase in Eimeria acervulina. Journal of Parasitology 72, 965–7.Google Scholar