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Genotyping and subtyping of Giardia and Cryptosporidium isolates from commensal rodents in China

Published online by Cambridge University Press:  12 January 2015

Z. ZHAO ,
R. WANG ,
W. ZHAO ,
M. QI ,
J. ZHAO ,
L. ZHANG ,
J. LI  and
A. LIU
Z. ZHAO
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China
R. WANG
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China
W. ZHAO
Affiliation:
Department of Parasitology, Harbin Medical University, Harbin 150081, PR China
M. QI
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China
J. ZHAO
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China
L. ZHANG*
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China
J. LI
Affiliation:
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China
A. LIU*
Affiliation:
Department of Parasitology, Harbin Medical University, Harbin 150081, PR China
*
* Corresponding authors. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China, Department of Parasitology, Harbin Medical University, Harbin 150081, PR China E-mail: [email protected] and E-mail: [email protected]
* Corresponding authors. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China, Department of Parasitology, Harbin Medical University, Harbin 150081, PR China E-mail: [email protected] and E-mail: [email protected]
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Summary

Cryptosporidium and Giardia are two important zoonotic intestinal parasites responsible for diarrhoea in humans and other animals worldwide. Rodents, as reservoirs or carriers of Cryptosporidium and Giardia, are abundant and globally widespread. In the present study, we collected 232 fecal specimens from commensal rodents captured in animal farms and farm neighbourhoods in China. We collected 33 Asian house rats, 168 brown rats and 31 house mice. 6·0% (14/232) and 8·2% (19/232) of these rodents were microscopy-positive for Giardia cysts and Cryptosporidium oocysts, respectively. All 14 Giardia isolates were identified as Giardia duodenalis assemblage G at a minimum of one or maximum of three gene loci (tpi, gdh and bg). By small subunit rRNA (SSU rRNA) gene sequencing, Cryptosporidium parvum (n = 12) and Cryptosporidium muris (n = 7) were identified. The gp60 gene encoding the 60-kDa glycoprotein was successfully amplified and sequenced in nine C. parvum isolates, all of which belonged to the IIdA15G1 subtype. Observation of the same IIdA15G1 subtype in humans (previously) and in rodents (here) suggests that rodents infected with Cryptosporidium have the potential to transmit cryptosporidiosis to humans.

Keywords

CryptosporidiumGiardiagenotypingsubtypingrodent
Type
Research Article
Information
Parasitology , Volume 142 , Issue 6 , May 2015 , pp. 800 - 806
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Copyright
Copyright © Cambridge University Press 2015 

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