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Genomic divergence in the ecologically differentiated English freshwater and marine strains of Pomphorhynchus laevis (Acanthocephala: Palaeacanthocephala): a preliminary investigation

Published online by Cambridge University Press:  06 April 2009

M. A. Munro ,
A. Reid  and
P. J. Whitfield
M. A. Munro
Affiliation:
Parasitology and Pest Biology Research Group, King's College London, Campden Hill Road, London W8 7AH
A. Reid
Affiliation:
Department of Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB
P. J. Whitfield
Affiliation:
Parasitology and Pest Biology Research Group, King's College London, Campden Hill Road, London W8 7AH
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Summary

In and around the British Isles, three biologically specialized strains of the fish acanthocephalan Pomphorhynchus laevis have been recognized, each differing in geographical distribution and both final and intermediate host utilization. Six restriction endonuclease digests of the genomic DNA of the English freshwater and marine strains were compared by genomic cross-hybridization using the randomly sheared and radio-isotope labelled genomic DNA of freshwater P. laevis, and also by using a heterologous isotope labelled ribosomal DNA probe. Autoradiographs of hybridized Southern blots provided evidence for considerable homogeneity in the total repetitive DNA of the two strains as well as some preliminary evidence for possible restriction fragment length polymorphisms using EcoR I and Pvu II.

Keywords

AcanthocephalaPomphorhynchus laevisDNAhybridization patterns
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 451 - 454
Copyright
Copyright © Cambridge University Press 1990

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