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Genomic and phenotypic diversity of Tunisian Theileria annulata isolates

Published online by Cambridge University Press:  06 April 2009

L. Ben Miled ,
K. Dellagi ,
G. Bernardi ,
T. R. Melrose ,
M. Darghouth ,
A. Bouattour ,
J. Kinnaird ,
B. Shiels ,
A. Tait  and
C. G. D. Brown
L. Ben Miled
Affiliation:
Laboratoire d’Hématologie et d' Immunopathologie, Faculté de Médecine de Tunis, Tunisie Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG, Scotland, U.K.
K. Dellagi
Affiliation:
Laboratoire d’Hématologie et d' Immunopathologie, Faculté de Médecine de Tunis, Tunisie
G. Bernardi
Affiliation:
Laboratoire d’Hématologie et d' Immunopathologie, Faculté de Médecine de Tunis, Tunisie
T. R. Melrose
Affiliation:
Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG, Scotland, U.K.
M. Darghouth
Affiliation:
École Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Tunisie
A. Bouattour
Affiliation:
École Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Tunisie
J. Kinnaird
Affiliation:
Wellcome Unit for Molecular Parasitology, Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QY, Scotland, U.K.
B. Shiels
Affiliation:
Wellcome Unit for Molecular Parasitology, Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QY, Scotland, U.K.
A. Tait
Affiliation:
Wellcome Unit for Molecular Parasitology, Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QY, Scotland, U.K.
C. G. D. Brown
Affiliation:
Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG, Scotland, U.K.
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Summary

This study describes polymorphism in Theileria annulata, an intracellular protozoan parasite of bovine leucocytes and red blood cells. Fifty-three different stocks of T. annulata, isolated from 17 sites (districts) in Tunisia, have been characterized by anti-parasite monoclonal antibody (MAb) reactivity, glucose phosphate isomerase (GPI) isoenzyme electrophoresis, and Southern blotting with two genomic DNA probes. There appears to be considerable diversity amongst T. annulata stocks from Tunisia, no two isolates being identical, even those from animals on the same farm. Two distinct antigenic populations were detected by MAb 7E7. They were defined by negative and positive cells in the indirect fluorescent antibody test. The percentage of positive cells in different isolates ranged between 0 and 100%. The population variation seen by GPI analysis and DNA probes was greater; 7 different GPI phenotypes were identified amongst the stocks studied, while DNA probes T. annulata Tunis (TaT) 17 and 21 detected up to 5 different variants. The majority of isolates were shown to contain more than one parasite population, the number of variants per isolate ranging from 1 to 4. No correlation between particular parasite phenotypes or genotypes and their geographical site of isolation was observed. Selection of parasite populations in vivo and in vitro is also discussed.

Keywords

Theileria annulatapolymorphismDNA probesisoenzymeelectrophoresismonoclonal antibodies
Type
Research Article
Information
Parasitology , Volume 108 , Issue 1 , January 1994 , pp. 51 - 60
Copyright
Copyright © Cambridge University Press 1994

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