Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-20T07:16:38.478Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic homogeneity within Leishmania (L.) infantum isolated from human and dogs: the relationship with the sandfly fauna distribution in endemic areas of Nueva Esparta State, Venezuela

Published online by Cambridge University Press:  22 February 2005

N. M. RODRIGUEZ ,
Z. DE GUGLIELMO ,
M. A. BARRIOS ,
R. M. BARRIOS ,
O. ZERPA  and
M. D. FELICIANGELI
N. M. RODRIGUEZ
Affiliation:
Genetic Engineering Laboratory, Institute of Biomedicine, Faculty of Medicine, Universidad Central de Venezuela, San Nicolas a Providencia, San José, Apdo 4043, Caracas 1010 A, Venezuela
Z. DE GUGLIELMO
Affiliation:
Genetic Engineering Laboratory, Institute of Biomedicine, Faculty of Medicine, Universidad Central de Venezuela, San Nicolas a Providencia, San José, Apdo 4043, Caracas 1010 A, Venezuela
M. A. BARRIOS
Affiliation:
Genetic Engineering Laboratory, Institute of Biomedicine, Faculty of Medicine, Universidad Central de Venezuela, San Nicolas a Providencia, San José, Apdo 4043, Caracas 1010 A, Venezuela
R. M. BARRIOS
Affiliation:
Genetic Engineering Laboratory, Institute of Biomedicine, Faculty of Medicine, Universidad Central de Venezuela, San Nicolas a Providencia, San José, Apdo 4043, Caracas 1010 A, Venezuela
O. ZERPA
Affiliation:
Genetic Engineering Laboratory, Institute of Biomedicine, Faculty of Medicine, Universidad Central de Venezuela, San Nicolas a Providencia, San José, Apdo 4043, Caracas 1010 A, Venezuela
M. D. FELICIANGELI
Affiliation:
Center for Biomedical Research, Faculty of Medicine, Universidad de Carabobo, Maracay, Aragua, Venezuela
Get access Rights & Permissions [Opens in a new window]

Abstract

Leishmania infantum has been described as a highly polymorphic group of parasites, responsible for visceral leishmaniasis and cutaneous leishmaniasis. In this paper we report the life-cycle of L. (L.) infantum in an endemic area of visceral leishmaniasis in Venezuela, by using molecular diagnosis and characterization of parasites isolated from dogs, humans with visceral leishmaniasis and sand flies. The molecular characterization was carried out by use of kDNA restriction analysis, dot-blot hybridization with species-specific probes and RFLP of the PCR products. The results demonstrated that L. (L.) infantum is the parasite responsible for VL in the island. The parasites were revealed to be genetically homogeneous with no intra-specific differences between isolates from different individuals. The highest homology of the isolates was with L. (L.) infantum from the Old World rather than with L. (L.) chagasi from the New World. Additionally, we report the geographical distribution of Lutzomyia longipalpis, and the relationship with the transmission of L. (L.) infantum in the studied area.

Keywords

Leishmania infantumLutzomyia longipalpishomogeneitymolecular characterization
Type
Research Article
Information
Parasitology , Volume 130 , Issue 6 , June 2005 , pp. 611 - 619
Copyright
© 2005 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

AGUILAR, C. M., FERNANDEZ, E., FERNANDEZ, R., CANNOVA, D. C., FERRER, E., CABRERA, Z., SOUZA, W. J. S. & COUTINHO, S. G. ( 1998). Urban visceral leishmaniasis in Venezuela. Memorias do Instituto Oswaldo Cruz 93, 15–16.CrossRefGoogle Scholar
ALEXANDER, B. & YOUNG, D. G. ( 1992). Dispersal of Phlebotomine sandflies (Diptera: Psychodidae) in a colombian focus of Leishmania (Viannia) braziliensis. Memorias do Instituto Oswaldo Cruz 87, 397–403.CrossRefGoogle Scholar
ARRIVILLAGA, J. C., RANGEL, Y., OVIEDO, M. & FELICIANGELI, M. D. ( 2000). Genetic diversity among Venezuelan populations of Lutzmoyia longipalpis (Diptera: Psychodidae: Phlebotominae). Journal of Medical Entomology 37, 325–330.CrossRefGoogle Scholar
BARRIOS, M. A., RODRIGUEZ, N., FELICIANGELI, M. D., ULRICH, M., TELLES, S., PINARDI, M. E. & CONVIT, J. ( 1994). Coexistence of two species of Leishmania in the digestive tract of the vector Lutzomyia ovallesi. American Journal of Tropical Medicine and Hygiene 51, 669–675.CrossRefGoogle Scholar
CUPOLILLO, E., GRIMALDI, G. & MOMEN, H. ( 1994). A general classification of New World Leishmania using numerical zymotaxonomy. American Journal of Tropical Medicine and Hygiene 57, 296–311.CrossRefGoogle Scholar
DELGADO, O., FELICIANGELI, M. D., GOMEZ, B., ALVARADO, J., GARCÍA, L. & BELLO, C. ( 1998). The re-emergence of American visceral leishmaniasis in an old focus in Venezuela: human and canine infection. Parasite 5, 317–323.CrossRefGoogle Scholar
FELICIANGELI, M. D., ZERPA, O., RODRIGUEZ, N., BRAVO, A., GALINDO, W. & CONVIT, J. ( 1998). Hallazgo de Lutzomyia longipalpis (Diptera: Psychodidae) naturalmente infectada con promastigotes en un foco endémico de leishmaniasis visceral en la isla de margarita, Venezuela. Boletin de la Division de Malariologia y Saneamiento Ambiental 38, 73–75.Google Scholar
FELICIANGELI, M. D., RODRIGUEZ, N. M., DE GUGLIELMO, Z. & RODRIGUEZ, A. ( 1999). The reemergence of American visceral leishmaniasis in an old focus in Venezuela. II. Vectors and parasites. Parasite 6, 113–120.Google Scholar
FELICIANGELI, M. D., MAZZARI, M. B., SAN BLAS, S. & ZERPA, O. ( 2003). Control trial of Lutzomyia longipalpis s.l in the island of Margarita, Venezuela. Tropical Medicine and International Health 8, 1131–1136.CrossRefGoogle Scholar
GRIMALDI, G., TESH, R. & McMAHON-PRAT, D. ( 1989). A review of geographic distribution and epidemiology of leishmaniasis in the New World. American Journal of Tropical Medicine and Hygiene 41, 687–725.CrossRefGoogle Scholar
GUERBOUJ, S., GUISANI, I., SPEYBROECK, N., LE RAY, D. & DUJARDIN, J. C. ( 2001). Genomic polymorphism of Leishmania infantum: a relationship with clinical pleomorphism? Infection. Genetic and Evolution 1, 49–59.CrossRefGoogle Scholar
HIDE, M, BAÑULS, A. L. & TIBAYRENC, M. ( 2001). Genetic heterogeneity and phylogenetic status of Leishmania (Leishmania) infantum Zymodeme MON-1: epidemiological implications. Parasitology 123, 425–432.CrossRefGoogle Scholar
LAMBSON, B., SMYTH, A. & BARKER, D. ( 1999). Sequence homology within a minicircle class of the Leishmania donovani complex. Molecular and Biochemical Parasitology 101, 229–232.CrossRefGoogle Scholar
LAMBSON, B., SMYTH, A. & BARKER, D. ( 2000). Development and characterization of a kinetoplast DNA probe and its use in the detection of parasites. Experimental Parasitology 94, 15–22.CrossRefGoogle Scholar
LAMPO, M., TORGERSON, D., MARQUEZ, L. M., RINALDI, M., GARCÍA, C. Z. & ARAB, A. ( 1999). Occurrence of sibling species of Lutzomyia longipalpis (Diptera: Psychodidae) in Venezuela: first evidence from reproductively isolated sympatric populations. American Journal of Tropical Medicine and Hygiene 64, 1004–1009.CrossRefGoogle Scholar
LANZARO, G. C., OSTROVSKA, K., HERRERO, M., LAWYER, P. G. & WARBURG, A. ( 1993). Lutzomyia longipalpis is a species complex: Genetic divergence and interspecific hybrid sterility among three populations. American Journal of Tropical Medicine and Hygiene 48, 839–847.CrossRefGoogle Scholar
LOPEZ, Y., OSORIO, L., ALVAREZ, G., ROJAS, J., JIMÉNEZ, F., GOMEZ, C. & FERRO, C. ( 1996). Sandfly Lutzomyia longipalpis in a cutaneous leishmaniasis focus in Central Colombia. Memorias do Instituto Oswaldo Cruz 97, 415–419.CrossRefGoogle Scholar
MANGABEIRA, O. ( 1969). Sobre a sistematica e biologia dos Phlebotomus do Ceara. Revista Brasileira de Malariologia e Doenças Tropicais 21, 3–26.Google Scholar
MAURICIO, I. L., HOWARD, M. K., STOHARD, J. R. & MILES, M. A. ( 1999). Genomic diversity in Leishmania donovani complex. Parasitology 119, 237–246.CrossRefGoogle Scholar
MAURICIO, I. L., STOHARD, J. R. & MILES, M. A. ( 2000). The strange case of Leishmania chagasi. Parasitology Today 16, 188–189.CrossRefGoogle Scholar
MUTEBI, J. P., TRIPET, F., ALEXANDER, J. B. & LANZARO, G. C. ( 2002). Genetic differentiation among populations of Lutzomyia longipalpis (Diptera: Psychodidae) in Central and South America. Annals of the Entomological Society of America 95, 740–752.CrossRefGoogle Scholar
MORENO, G., RIOUX, J. A., LANOTTE, G., PRATLONG, F. & SERRES, E. ( 1986). Le complexe Leishmania donovani s.l. Analyse enzimatique et traitment numerique individualisation du complexe Leishmania infantum. Corollaires biogeographiques et phyletiques. A propos de 146 souches originaires de l'Ancien et du Nouveau Monde. In coll Int CNRS/INSERM 1984, Leishmania Taxonomie et Phylogenese Applications eco-epidemiologiques, IMEEE. Montpellier, pp. 105–117.
MORENO, G., SCORZA, J. V. & AÑEZ, N. ( 1990). Leishmania infantum en el estado Trujillo, Venezuela. Acta Científica Venezolana 41, 27.Google Scholar
LAINSON, R., SHAW, J. J., RYAN, L., RIBEIRO, R. S. & SILVEIRA, F. T. ( 1985). Leishmaniasis in Brazil XXI. Visceral leishmaniasis in the Amazonan region and further observations on role of Lutzomyia longipalpis (Lutz and Neiva, 1912) as the vector. Transactions of the Royal Society of Tropical Medicine and Hygiene 79, 223–226.Google Scholar
RODRÍGUEZ, N., DE LIMA, H., RODRÍGUEZ, A., BREWSTER, S. & BARKER, D. C. ( 1997). Genomic DNA repeat from Leishmania (Viannia) braziliensis (Venezuelan strain) containing repeats and microsatellites. Parasitology 5, 349–358.CrossRefGoogle Scholar
RODRIGUEZ, N., RODRIGUEZ, A., CARDONA, M., BARRIOS, M. A., McCANN, S. H. & BARKER, D. C. ( 2000): Leishmania (Viannia) guyanensis: A new minicircle class exclusive to this species isolated from a DNA cosmid library useful for taxonomic purposes. Experimental Parasitology 94, 143–149.CrossRefGoogle Scholar
RODRIGUEZ, N., DE LIMA, H., AGUILAR, C. M., RODRIGUEZ, A., BARKER, D. C. & CONVIT, J. ( 2002). Molecular epidemiology of cutaneous leishmaniasis in Venezuela. Transactions of the Royal Society of Tropical Medicine and Hygiene 96, 105–109.CrossRefGoogle Scholar
TESH, R. B. ( 1995). Control of zoonotic visceral leishmaniasis: Is it to change strategies? American Journal of Tropical Medicine and Hygiene 52, 287–292.Google Scholar
TRAVI, B. L., VELEZ, J. D., BRUTUS, L., SEGURA, I., JARAMILLO, C. & MONTOYA, J. ( 1990). Lutzomyia evansi, an alternate vector of Leishmania chagasi in a Colombian focus of visceral leishmaniasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 84, 676–677.CrossRefGoogle Scholar
URIBE, B. ( 1999). The status of the Lutzomyia longipalpis species complex and possible implications for Leishmania transmission. Memorias do Instituto Oswaldo Cruz 94, 729–734.CrossRefGoogle Scholar
VICTOIR, K., DUJARDIN, J. C., DE DONKER, S., BARKER, D. C., AREVALO, J., HAMERS, R. & LERAY, D. ( 1995). Plasticity of GP63 gene organization in Leishmania (V) braziliensis and Leishmania (V) peruviana. Parasitology 111, 265–273.CrossRefGoogle Scholar
WARD, R. D. ( 1985). Vector biology and control. In Leishmaniasis (ed. Chang, K. P. & Bray, R.), pp. 199–212. Elsevier Science Publishers, Amsterdam, New York and Oxford.
YOUNG, D. G. & DUNCAN, M. A. ( 1994). Guide to the identification and geographic distribution of Lutzomyia sandflies in Mexico, the West Indies, Central and South America. (Diptera: Psychodidae). Memoirs of the American Entomologist Institute 54, 1–881. Associated Publishers, Gainsville, FL.Google Scholar
ZERPA, O., ULRICH, M., NEGRON, E., RODRÍGUEZ, N., CENTENO, M., RODRÍGUEZ, V., BARRIOS, R. M., BELISARIO, D., REED, S. & CONVIT, J. ( 2000). Canine visceral leishmaniasis in Margarita Island (Nueva Esparta, Venezuela). Transactions of the Royal Society of Tropical Medicine and Hygiene 94, 484–487.CrossRefGoogle Scholar
ZERPA, O., PRATLONG, F., ULRICH, M. & CONVIT, J. ( 2001). Isolation of Leishmania infantum MON-1 from canine and human visceral leishmaniasis on Margarita Island, Venezuela. Memorias Instituto Oswaldo Cruz 96, 901–902.CrossRefGoogle Scholar
ZERPA, O., ULRICH, M., BENITEZ, M., AVILA, C., RODRÍGUEZ, V., CENTENO, M., BELISARIO, D., REED, S. & CONVIT, J. ( 2002). Epidemiological and immunological aspects of human visceral leishmaniasis on Margarita island, Venezuela. Memorias Instituto Oswaldo Cruz 97, 1079–1083.CrossRefGoogle Scholar
ZULUETA, A. M., VILLARROEL, E., RODRÍGUEZ, N., FELICIANGELI, M. D., MASSARRI, M., REYES, O., RODRÍGUEZ, V., CENTENO, M., BARRIOS, R. M. & ULRICH, M. ( 1999). Epidemiologic aspects of American visceral leishmaniasis in an endemic focus in Eastern Venezuela. American Journal of Tropical Medicine and Hygiene 61, 945–950.CrossRefGoogle Scholar