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Generation and characterization of U937-TR: a platform cell line for inducible gene expression in human macrophages

Published online by Cambridge University Press:  27 July 2020

Cristian Camilo Galindo Open the ORCID record for Cristian Camilo Galindo [Opens in a new window]  and
Carlos Arturo Clavijo-Ramírez Open the ORCID record for Carlos Arturo Clavijo-Ramírez [Opens in a new window]
Cristian Camilo Galindo*
Affiliation:
Universidad Nacional de Colombia, Sede Bogotá–Facultad de Medicina, Grupo de investigación: Biología Celular y Molecular de Parásitos y Hospederos, Laboratorio de Parasitología, Carrera 30 No. 45-03, Edificio 471, Oficina: 318, Piso 3, Bogotá, Código111321, Colombia
Carlos Arturo Clavijo-Ramírez
Affiliation:
Departamento de Biología, Universidad Nacional de Colombia, Sede Bogotá–Facultad de Ciencias, Grupo de investigación: Biología Celular y Molecular de Parásitos y Hospederos, Carrera 30 No. 45-03, Edificio 421, Oficina: 211, Piso 2, Bogotá, Código111321, Colombia
*
Author for correspondence: Cristian Camilo Galindo, E-mail: [email protected]
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Abstract

Monocytes and macrophages are involved in a wide range of biological processes and parasitic diseases. The characterization of the molecular mechanisms governing such processes usually requires precise control of the expression of genes of interest. We implemented a tetracycline-controlled gene expression system in the U937 cell line, one of the most used in vitro models for the research of human monocytes and macrophages. Here we characterized U937-derived cell lines in terms of phenotypic (morphology and marker expression) and functional (capacity for phagocytosis and for Leishmania parasite hosting) changes induced by phorbol-12-myristate-13-acetate (PMA). Finally, we provide evidence of tetracycline-inducible and reversible Lamin-A gene silencing of the PMA-differentiated U937-derived cells.

Keywords

Human macrophageinducible gene expressionU937tetracycline
Type
Research Article
Information
Parasitology , Volume 147 , Issue 13 , November 2020 , pp. 1524 - 1531
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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