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A female-specific cDNA sequence of Schistosoma mansoni encoding a mucin-like protein that is expressed in the epithelial cells of the reproductive duct*

Published online by Cambridge University Press:  06 April 2009

M. Menrath ,
A. Michel  and
W. Kunz
M. Menrath
Affiliation:
Institute of Geneticsand Biological-Medical Research Center, Heinrich-Heine-University, Universitäts-Strasse 1, D-40225 Düsseldorf, Germany
A. Michel
Affiliation:
Institute of Geneticsand Biological-Medical Research Center, Heinrich-Heine-University, Universitäts-Strasse 1, D-40225 Düsseldorf, Germany
W. Kunz
Affiliation:
Institute of Geneticsand Biological-Medical Research Center, Heinrich-Heine-University, Universitäts-Strasse 1, D-40225 Düsseldorf, Germany
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Summary

Seven cDN A clones of Schistosoma mansoni containing the C-terminal part of the deduced sequence of a mucin-like protein have been identified. The protein contains 28% threonines, 20% serines, and has a pi of 3middot;4. On Northern blots of RNA of adult worms, the cDNA clones detect 2 transcripts of 1middot;65 and 4middot;2 kb which are expressed only in female worms. The tissue of gene expression, as revealed by in situ hybridization, is the epithelium surrounding the female reproduction duct proximal to its entrance into the ootype. Accumulation of N-glycosylation sites suggests that the protein, like other mucins, might form a protective layer, coating the lining of the duct. Regarding its acidic pi, we hypothesize a role in preventing premature egg-shell formation. This is the first female-specifically transcribed sequence, hitherto known in S. mansonithat is not expressed in the vitellaria.

Keywords

Schistosoma mansonimucin-like proteinfemale-specific cDNA sequence
Type
Research Article
Information
Parasitology , Volume 111 , Issue 4 , November 1995 , pp. 477 - 483
Copyright
Copyright © Cambridge University Press 1995

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References

REFERENCES

Armstrong, J. C. (1965). Mating behaviour and development of schistosomes in the mouse. Journal of Parasitology 51, 605–16.Google Scholar
Bennett, J. L., Seed, J. L. & Boff, M. (1978). Fluorescent histochemical localization of phenol oxidase in female Schistosoma mansoni. Journal of Parasitology 64, 941–4.CrossRefGoogle ScholarPubMed
Bobek, L. A., Rekosh, D. M., Van Keulen, H. & Loverde, P. T. (1986). Characterization of a female-specific cDNA derived from a developmentally regulated mRNA in the human blood fluke Schistosoma mansoni. Proceedings of the National Academy of Sciences, USA 83, 5544–8.CrossRefGoogle ScholarPubMed
Chirgwin, J. M., Przybyla, A. E., Macdonald, R. J. & Rutter, W. J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294–9.CrossRefGoogle ScholarPubMed
Clough, E. R. (1981). Morphology of reproductive organs and oogenesis in bisexual and unisexual transplants of mature Schistosoma mansoni females. Journal of Parasitology 67, 535–9.CrossRefGoogle ScholarPubMed
Dietzel, J., Hirzmann, J., Preis, D., Symmons, P. & Kunz, W. (1992). Ferritins of Schistosoma mansoni: Sequence comparison and expression in female and male worms. Molecular and Biochemical Parasitology 50, 245–54.CrossRefGoogle ScholarPubMed
Duvall, R. H. & Dewitt, W. D. (1967). An improved perfusion technique for recovering adult schistosomes from laboratory animals. American Society of Tropical Medicine and Hygiene 16, 483–6.CrossRefGoogle ScholarPubMed
Finken, M., Sobek, A., Symmons, P. & Kunz, W. (1994). Characterization of the complete protein disulfide isomerase gene of Schistosoma mansoni and identification of the tissues of its expression. Molecular and Biochemical Parasitology 64, 135–44.CrossRefGoogle ScholarPubMed
Gönnert, R. (1955). Schistosomiasis Studien. Zeitschrift für Tropenmedizin 6, 18–33.Google Scholar
Gum, J. R., Byrd, J. C., Hicks, J. W., Toribara, N. W., Lamport, D. T. & Kim, Y. S. (1989). Molecular cloning of human intestinal mucin cDNAs. Sequence analysis and evidence for genetic polymorphism. Journal of Biological Chemistry 264, 6480–7.CrossRefGoogle ScholarPubMed
Hoffmann, W. (1988). A new repetitive protein from Xenopus laevis skin highly homologous to pancreatic spasmolytic polypeptide. Journal of Biological Chemistry 263, 7686–90.Google Scholar
Johnson, K. S., Taylor, D. W. & Cordingley, J. S. (1987). Possible eggshell protein gene from Schistosoma mansoni. Molecular and Biochemical Parasitology 22, 89–100.Google Scholar
Köster, B., Dargatz, H., Schröder, J., Hirzmann, J., Haarmann, C., Symmons, P. & Kunz, W. (1988). Identification and localisation of the products of a putative eggshell precursor gene in the vitellarium of Schistosoma mansoni. Molecular and Biochemical Parasitology 31, 183–98.CrossRefGoogle ScholarPubMed
Kunz, W., Gohr, L., Grevelding, C., Schüssler, P., Sommer, G., Menrath, M. & Michel, A. (1995). Schistosoma mansoni: Control of female fertility by the male. Memorias do Institute Oswaldo Cruz 90, 185–9.CrossRefGoogle ScholarPubMed
Kunz, W., Opatz, K., Finken, M. & Symmons, P. (1987). Sequences of two genomic fragments containing an identical coding region for a putative eggshell precursor protein of Schistosoma mansoni. Nucleic Acids Research 15, 5894.Google Scholar
Lasky, L. A., Singer, M. S., Dowbenko, D., Imai, Y., Henzel, W. J., Grimley, C., Fennie, C., Gillett, N., Watson, S. R. & Rosen, S. D. (1992). An endothelial ligand for L-selectin is a novel mucin-like molecule. Cell 69, 927–38.CrossRefGoogle ScholarPubMed
Loverde, P. T. & Chen, L. (1991). Schistosome female reproductive development. Parasitology Today 7, 303–8.CrossRefGoogle ScholarPubMed
Martin, C. H., Mayeda, C. A. & Meyerowitz, E. M. (1988). Evolution and expression of the Sgs-3 glue gene of Drosophita. Journal of Molecular Biology 201, 273–87.Google Scholar
Pena, S. D. J., Macedo, A. M., Braga, V. M. M., Rumjanek, F. D. & Simpson, A. J. G. (1990). F10, the gene for the glycine-rich major eggshell protein of Schistosoma mansoni recognizes a family of hypervariable minisatellites in the human genome. Nucleic Acids Research 18, 7466.CrossRefGoogle ScholarPubMed
Reis, M. G., Kuhns, J., Blanton, R. & Davis, A. H. (1989). Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni. Molecular and Biochemical Parasitology 32, 113–20.CrossRefGoogle ScholarPubMed
Schweinfest, C. W., Henderson, K. W., Gu, J.-R., Kottaridis, S. D., Besbeas, S., Panotopoulou, E. & Papas, T. S. (1990). Subtraction hybridization cDNA libraries from colon carcinoma and hepatic cancer. Genetic Analysis: Techniques and Application 7, 64–70.CrossRefGoogle ScholarPubMed
Seed, J. L., Boff, M. & Bennett, J. L. (1978). Phenol oxidase activity: induction in female schistosomes by in vitro incubation. Journal of Parasitology 64, 283–9.CrossRefGoogle ScholarPubMed
Strous, G. J. & Dekker, J. (1992). Mucin-type glycoproteins. Critical Reviews of Biochemistry and Molecular Biology 27, 57–92.CrossRefGoogle ScholarPubMed
Swaarop, A., Xu, J., Agarwal, N. & Weissman, S. M. (1991). A simple and efficient cDNA library subtraction procedure: isolation of human retina-specific cDNA clones. Nucleic Acids Research 19, 1954.CrossRefGoogle Scholar
Toribara, N. W., Gum, J. R. Jr., Culhane, P. J., Lagace, R. E., Hicks, J. W., Petersen, C. M. & Kim, Y. S. (1991). MUC-2 human small intestinal mucin gene structure. Repeated arrays and polymorphism. Journal of Clinical Investigation 88, 1005–13.Google Scholar
Wells, K. E. & Cordingley, J. S. (1991). Schistosoma mansoni: Eggshell formation is regulated by pH and calcium. Experimental Parasitology 73, 295–310.CrossRefGoogle ScholarPubMed
West, S., Schröder, J. & Kunz, W. (1990). A multiple-staining procedure for the detection of different DNA fragments on a single blot. Analytical Biochemistry 190, 254–8.CrossRefGoogle ScholarPubMed