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Expression of NF-Y nuclear factor in Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

E. Serra ,
J.-L. Liu ,
A. Capron ,
K. Zemzoumi  and
C. Dissous
E. Serra
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
J.-L. Liu
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
A. Capron
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
K. Zemzoumi
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
C. Dissous*
Affiliation:
Unité INSERM 167, Institut Pasteur Lille, 1 rue du Professeur Calmette, 59019 Lille, France
*
*Corresponding author. Tel: + 33 20 877350. Fax: + 33 20 877888.
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Summary

The A subunit of NF-Y nuclear factor from Schistosoma mansoni was expressed in E. coli fused to a histidine tag and purified by affinity chromatography using a Ni2+-Agarose matrix. Antibodies against the recombinant protein were prepared and used for Western blot and immunolocalization. The presence of SMNF-YA in all stages of the parasite life-cycle was determined by RT-PCR and Western blot analysis. The immunolocalization of SMNF-YA showed the presence of this factor in a parenchymal cell population of cercariae and adult worms and in embryos within eggs. The expression of SMNF-YA was demonstrated to decrease in maturating spermatozoites whereas an accumulation of this factor was observed in the nucleus from oocytes during their maturation processes.

Keywords

Schistosoma mansoniNF-Y nuclear factorimmunolocalizationoocytes
Type
Research Article
Information
Parasitology , Volume 113 , Issue 5 , November 1996 , pp. 457 - 464
Copyright
Copyright © Cambridge University Press 1996

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