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Expression, characterization and crystal structure of thioredoxin from Schistosoma japonicum

Published online by Cambridge University Press:  26 March 2015

YONGDONG LI ,
PAN LI ,
YUN PENG ,
QUNFENG WU ,
FUYAN HUANG ,
XIANG LIU ,
XUN LI ,
HUI ZHOU ,
DAOYI GUO  and
DASHUANG SHI
...Show all authors
YONGDONG LI
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, People's Republic of China Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China Key Laboratory on Biology of Parasite and Vector, Ministry of Health, and WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, People's Republic of China
PAN LI
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China
YUN PENG
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China
QUNFENG WU
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, People's Republic of China Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China Key Laboratory on Biology of Parasite and Vector, Ministry of Health, and WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, People's Republic of China
FUYAN HUANG
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China
XIANG LIU
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China
XUN LI
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China
HUI ZHOU
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China
DAOYI GUO
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China
DASHUANG SHI
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China Center for Genetic Medicine Research and Department of Integrative Systems Biology, Children's National Medical Center, The George Washington University, Washington, District of Columbia 20010, USA
XIAO-NONG ZHOU*
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, People's Republic of China Key Laboratory on Biology of Parasite and Vector, Ministry of Health, and WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, People's Republic of China
XIAOLIN FAN*
Affiliation:
Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China
*
* Corresponding authors. Key Laboratory on Biology of Parasite and Vector, Ministry of Health, and WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, People's Republic of China, Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China. E-mail: [email protected] and [email protected]
* Corresponding authors. Key Laboratory on Biology of Parasite and Vector, Ministry of Health, and WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, People's Republic of China, Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Chemistry and Chemical Engineering College, Gannan Normal University, Ganzhou 341000, People's Republic of China. E-mail: [email protected] and [email protected]
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Summary

Schistosoma japonicum, a human blood fluke, causes a parasitic disease affecting millions of people in Asia. Thioredoxin–glutathione system of S. japonicum plays a critical role in maintaining the redox balance in parasite, which is a potential target for development of novel antischistosomal agents. Here we cloned the gene of S. japonicum thioredoxin (SjTrx), expressed and purified the recombinant SjTrx in Escherichia coli. Functional assay shows that SjTrx catalyses the dithiothreitol (DTT) reduction of insulin disulphide bonds. The coupling assay of SjTrx with its endogenous reductase, thioredoxin glutathione reductase from S. japonicum (SjTGR), supports its biological function to maintain the redox homeostasis in the cell. Furthermore, the crystal structure of SjTrx in the oxidized state was determined at 2·0 Å resolution, revealing a typical architecture of thioredoxin fold. The structural information of SjTrx provides us important clues for understanding the maintenance function of redox homeostasis in S. japonicum and pathogenesis of this chronic disease.

Keywords

thioredoxincrystal structureSchistosoma japonicum
Type
Research Article
Information
Parasitology , Volume 142 , Issue 8 , July 2015 , pp. 1044 - 1052
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Copyright
Copyright © Cambridge University Press 2015 

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