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Exploring Neotropical anuran parasites: a morphological, life cycle and phylogenetic study of Catadiscus marinholutzi (Trematoda: Diplodiscidae)

Published online by Cambridge University Press:  17 February 2021

Murilo S. Queiroz ,
Philippe V. Alves ,
Danimar López-Hernández ,
Luciano A. Anjos  and
Hudson A. Pinto Open the ORCID record for Hudson A. Pinto [Opens in a new window]
Murilo S. Queiroz
Affiliation:
Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Postal Code 18618-970, Botucatu, SP, Brazil
Philippe V. Alves
Affiliation:
Departmento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, P.O. Box 486, Postal Code 31270-901, Belo Horizonte, MG, Brazil
Danimar López-Hernández
Affiliation:
Departmento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, P.O. Box 486, Postal Code 31270-901, Belo Horizonte, MG, Brazil
Luciano A. Anjos
Affiliation:
Departamento de Biologia e Zootecnia, Universidade Estadual Paulista, Passeio Monção 226, Postal Code 15385-000, Ilha Solteira, SP, Brazil
Hudson A. Pinto*
Affiliation:
Departmento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, P.O. Box 486, Postal Code 31270-901, Belo Horizonte, MG, Brazil
*
Author for correspondence: Hudson A. Pinto, E-mail: [email protected]
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Abstract

Amphistome species belonging to the genus Catadiscus are poorly studied intestinal trematodes found primarily in Neotropical anurans. Herein, developmental stages of an amphistome species found during herpetological and malacological surveys in a temporary marsh pond from Brazil were subjected to morphological (light and scanning electron microscopy) and molecular analyses. Adult parasites recovered from anurans were identified as Catadiscus marinholutzi. Amphistome cercariae found in the planorbid snails Drepanotrema depressissimum and Drepanotrema lucidum from the same waterbody were used for experimental and molecular studies. Immature parasites, morphologically compatible with members of Catadiscus, were experimentally obtained in laboratory-reared tadpoles. Sequencing of a partial region of 28S rDNA gene of both adult and cercariae revealed 100% similarity between these developmental stages, confirming their conspecificity. Phylogenetic analyses were attempted for the first time to reveal the position of a species of Catadiscus in the superfamily Paramphistomoidea. Catadiscus marinholutzi falls in a virtual polytomy together with other paramphistomoids, which leaves its phylogenetic relationships within the group unclear. Moreover, the high genetic divergence to Diplodiscus spp. (10.06–10.84%) cast doubts on the placement of Catadiscus within Diplodiscidae. Hence the species composition of the Diplodiscidae should be re-evaluated in further studies using a broader spectrum of related taxa.

Keywords

AamphibiansBrazilcercariaelife cycleParamphistomoideaphylogenetic relationshipssnailtrematodes
Type
Research Article
Information
Parasitology , Volume 148 , Issue 7 , June 2021 , pp. 798 - 808
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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