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Existence of host-related DNA sequences in the schistosome genome

Published online by Cambridge University Press:  06 April 2009

Y. Iwamura ,
Y. Irie ,
R. Kominami ,
T. Nara  and
K. Yasuraoka
Y. Iwamura
Affiliation:
Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305, Japan
Y. Irie
Affiliation:
Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305, Japan
R. Kominami
Affiliation:
School of Medicine, Niigata University, Niigata-shi, Niigata 951, Japan
T. Nara
Affiliation:
Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305, Japan
K. Yasuraoka
Affiliation:
Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki 305, Japan
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Extract

DNA sequences homologous to the mouse intracisternal A particle and endogenous type C retrovirus were detected in the DNAs of Schistosoma japonicum adults and S. mansoni eggs. Furthermore, other kinds of repetitive sequences in the host genome such as mouse type 1 Alu sequence (B1), mouse type 2 Alu sequence (B2) and mo-2 sequence, a mouse mini-satellite, were also detected in the DNAs from adults and eggs of S. japonicum and eggs of S. mansoni. Almost all of the sequences described above were absent in the DNAs of S. mansoni adults. The DNA fingerprints of schistosomes, using the mo-2 sequence, were indistinguishable from each other and resembled those of their murine hosts. Moreover, the mo-2 sequence was hypermethylated in the DNAs of schistosomes and its amount was variable in them. These facts indicate that host-related sequences are actually present in schistosomes and that the mo-2 repetitive sequence exists probably in extra-chromosome.

Keywords

Schistosoma japonicumSchistosoma mansonihost-related sequencesrepetitive sequencesmini-satellite (mo-2)
Type
Research Article
Information
Parasitology , Volume 102 , Issue 3 , June 1991 , pp. 397 - 403
Copyright
Copyright © Cambridge University Press 1991

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