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Evasion of macrophage microbicidal mechanisms by mature sporozoites of Plasmodium yoelii yoelii

Published online by Cambridge University Press:  06 April 2009

Judith E. Smith  and
James Alexander
Judith E. Smith
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, Prince Consort Road, London SW7 2BB
James Alexander
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science and Technology, Prince Consort Road, London SW7 2BB
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Summary

Sporozoites of Plasmodium yoelii yoelii were incubated for 40 min with BALB/c peritoneal macrophages in the presence of nitro-blue tetrazolium (NBT). While immature oocyst sporozoites triggered the macrophage respiratory burst, as visualized microscopically by the localized reduction of NBT to insoluble formazan, 97·6% of mature salivary gland sporozoites did not induce such a response. The macrophage oxidative response was also induced by 82·7% of heat-inactivated and 95·7% of trypsin-treated salivary gland sporozoites. The relationship of these results to the infectivity and immunogenicity of malarial sporozoites is discussed.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 1 , August 1986 , pp. 33 - 38
Copyright
Copyright © Cambridge University Press 1986

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