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Evaluation of immune responses raised against Tc13 antigens of Trypanosoma cruzi in the outcome of murine experimental infection

Published online by Cambridge University Press:  09 November 2007

G. A. GARCÍA ,
M. R. ARNAIZ ,
M. I. ESTEVA ,
S. A. LAUCELLA ,
P. A. GARAVAGLIA ,
S. E. IBARRA  and
A. M. RUIZ
G. A. GARCÍA*
Affiliation:
Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben” – ANLIS/Malbrán, Paseo Colón 568 (cp: 1063), Buenos Aires, Argentina
M. R. ARNAIZ
Affiliation:
Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben” – ANLIS/Malbrán, Paseo Colón 568 (cp: 1063), Buenos Aires, Argentina
M. I. ESTEVA
Affiliation:
Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben” – ANLIS/Malbrán, Paseo Colón 568 (cp: 1063), Buenos Aires, Argentina
S. A. LAUCELLA
Affiliation:
Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben” – ANLIS/Malbrán, Paseo Colón 568 (cp: 1063), Buenos Aires, Argentina
P. A. GARAVAGLIA
Affiliation:
Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben” – ANLIS/Malbrán, Paseo Colón 568 (cp: 1063), Buenos Aires, Argentina
S. E. IBARRA
Affiliation:
Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben” – ANLIS/Malbrán, Paseo Colón 568 (cp: 1063), Buenos Aires, Argentina
A. M. RUIZ
Affiliation:
Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben” – ANLIS/Malbrán, Paseo Colón 568 (cp: 1063), Buenos Aires, Argentina
*
*Corresponding author. Tel: +54 11 4331 2330. Fax: +54 11 4331 7142. E-mail: [email protected]
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Summary

We have previously reported that genetic immunization with Tc13Tul antigen of Trypanosoma cruzi, the aetiological agent of Chagas' disease, triggers harmful effects and non-protective immune responses. In order to confirm the role of Tc13 antigens during T. cruzi infection, herein we studied the humoral and cellular immune responses to the Tc13Tul molecule and its EPKSA C-terminal portion in BALB/c T. cruzi-infected mice or mice immunized with recombinant Tc13Tul. Analysis of the antibody response showed that B-cell epitopes that stimulate a sustained IgM production along the infection and high levels of IgG in the acute phase are mainly located at the Tc13 N- and C-terminal domains, respectively. DTH assays showed that T-cell epitopes are mainly at the Tc13 N-terminal segment and that they do not elicit an efficient memory response. Recombinant Tc13Tul did not induce IFN-γ secretion in either infected or immunized mice. However, a putative CD8+Tc13Tul-derived peptide was found to elicit IFN-γ production in chronically infected animals. Immunization with recombinant Tc13Tul did not induce pathology in tissues and neither did it protect against the infection. Our results show that in the outcome of T. cruzi infection the Tc13 family protein mainly triggers non-protective immune responses.

Keywords

Chagas' diseasetrans-sialidaseimmunizationpathology
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 3 , March 2008 , pp. 347 - 357
Copyright
Copyright © Cambridge University Press 2007

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