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Epidemiological relationships of Trypanosoma brucei stocks from South East Uganda: evidence for different population structures in human infective and non-human infective isolates

Published online by Cambridge University Press:  06 April 2009

G. Hide ,
S. C. Welburn ,
A. Tait  and
I. Maudlin
G. Hide
Affiliation:
Wellcome Unit of Molecular Parasitology, Department of Veterinary Parasitology, Glasgow University Veterinary School, Bearsden Road, Glasgow G61 IQH
S. C. Welburn
Affiliation:
Tsetse Research Laboratory, ODA/University of Bristol, Department of Veterinary Medicine, Langford House, Langford, Bristol BS18 7DU
A. Tait
Affiliation:
Wellcome Unit of Molecular Parasitology, Department of Veterinary Parasitology, Glasgow University Veterinary School, Bearsden Road, Glasgow G61 IQH
I. Maudlin
Affiliation:
Tsetse Research Laboratory, ODA/University of Bristol, Department of Veterinary Medicine, Langford House, Langford, Bristol BS18 7DU
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Summary

This study represents an analysis of trypanosome strains circulating within a confined location over a short period of time during a sleeping sickness epidemic in S.E. Uganda. A large number of Trypanosoma brucei isolates (88) were collected from a variety of hosts (man, cattle, pigs and tsetse) from villages within a 10 km radius and were analysed for variation in isoenzyme patterns, restriction fragment length polymorphism (RFLP) in repetitive DNA sequences and susceptibility to human serum. The human infective stocks form a clearly distinguishable population when compared with other stocks circulating in the domestic cattle reservoir. The data here support the occurrence of genetic exchange between the cattle stocks while an ‘epidemic’ population structure involving limited genetic exchange is a characteristic of the human infective stocks. Furthermore, it is shown that when both RFLP and isoenzyme analysis are carried out most stocks appear to have individual genotypes. Stocks which were formerly grouped as zymodemes are better considered as a collection of distinct individuals.

Keywords

KinetoplastidaTrypanosoma bruceimolecular epidemiologyhuman infectivityclonalitygenetic exchange
Type
Research Article
Information
Parasitology , Volume 109 , Issue 1 , July 1994 , pp. 95 - 111
Copyright
Copyright © Cambridge University Press 1994

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