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Electron microscopic observation of cytoskeletal frame structures and detection of tubulin on the apical region of Cryptosporidium parvum sporozoites

Published online by Cambridge University Press:  27 November 2007

M. MATSUBAYASHI ,
H. TAKASE ,
I. KIMATA ,
H. NAKAGAWA ,
H. TANI ,
K. SASAI  and
E. BABA
M. MATSUBAYASHI
Affiliation:
Department of Food and Nutrition, Osaka Yuhigaoka Gakuen Junior College, Tennoji-ku, Osaka 543-0073, Japan
H. TAKASE
Affiliation:
Hanaichi Ultrastructure Research Institute, Okazaki, Aichi 444-0076, Japan
I. KIMATA
Affiliation:
Department of Protozoal Diseases, Graduate School of Medicine, Osaka City University, Abeno-ku, Osaka 545-8585, Japan
H. NAKAGAWA
Affiliation:
Department of Central Laboratory, Graduate School of Medicine, Osaka City University, Abeno-ku, Osaka 545-8585, Japan
H. TANI
Affiliation:
Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan
K. SASAI*
Affiliation:
Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan
E. BABA
Affiliation:
Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan
*
*Corresponding author: Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. Tel: +81 72 254 9506. Fax: +81 72 254 9918. E-mail: [email protected]
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Summary

Cryptosporidium parvum is an intracellular protozoan parasite belonging to the phylum Apicomplexa, and a major cause of waterborne gastroenteritis throughout the world. Invasive zoites of apicomplexan parasites, including C. parvum, are thought to have characteristic organelles on the apical apex; however, compared with other parasites, the cytoskeletal ultrastructure of C. parvum zoites is poorly understood. Thus, in the present study, we ultrastructurally examined C. parvum sporozoites using electron microscopy to clarify the framework of invasive stages. Consequently, at the apical end of sporozoites, 3 apical rings and an electron-dense collar were seen. Two thick central microtubules were seen further inside sporozoites and extended to the posterior region. Using anti-α and -β tubulin antibodies generated from sea urchin and rat brain, both antibodies cross-reacted at the apical region of sporozoites in immunofluorescent morphology. The molecular mass of C. parvum α tubulin antigen was 50 kDa by Western blotting and the observed apical cytoskeletal structures were shown to be composed of α tubulin by immunoelectron microscopy. These results suggested that C. parvum sporozoites were clearly different in their cytoskeletal structure from those of other apicomplexan parasites.

Keywords

apicalcomplexCryptosporidiumcytoskeletonsporozoitetubulin
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 3 , March 2008 , pp. 295 - 301
Copyright
Copyright © Cambridge University Press 2007

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