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Eimeria tenella: in vitro development in irradiated bovine kidney cells

Published online by Cambridge University Press:  06 April 2009

M. St J. Crane ,
D. M. Schmatz ,
S. Stevens ,
Mary C. Habbersett  and
P. K. Murray
M. St J. Crane
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
D. M. Schmatz
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
S. Stevens
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
Mary C. Habbersett
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
P. K. Murray
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
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Summary

The initial infection and first-generation development of Eimeria tenella was quantified using a cloned MDBK (Madin-Darby Bovine Kidney) cell line, irradiated with gamma radiation prior to infection, as the host cell. Irradiated cell cultures were found to be more susceptible to infection and had a greater capacity to support parasite development than non-irradiated cultures. It was suggested that the larger proportion of cells in the G2 phase of the cell cycle, the larger individual cell size and the inhibition of cell division in the irradiated cultures were all factors contributing to the increased susceptibility to infection and capacity to support parasite growth and development. The application of this technique (host cell irradiation) to the cultivation of other intracellular, protozoan parasites is discussed.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 3 , June 1984 , pp. 521 - 530
Copyright
Copyright © Cambridge University Press 1984

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References

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