Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-19T05:13:03.352Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of high-fat chow on heart tissue in acute and chronic experimental murine schistosomiasis mansoni

Published online by Cambridge University Press:  19 July 2012

V. C. GÓES ,
R. H. NEVES ,
A. C. M. B. ALENCAR ,
A. V. OLIVEIRA ,
D. C. GOMES  and
J. R. MACHADO-SILVA
V. C. GÓES
Affiliation:
Laboratório de Helmintologia Romero Lascasas Porto, Disciplina de Parasitologia, Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ 20550-170, Brasil
R. H. NEVES
Affiliation:
Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
A. C. M. B. ALENCAR
Affiliation:
Laboratório de Helmintologia Romero Lascasas Porto, Disciplina de Parasitologia, Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ 20550-170, Brasil
A. V. OLIVEIRA
Affiliation:
Disciplina de Anatomia Patológica, Departamento de Patologia e Laboratórios, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil
D. C. GOMES
Affiliation:
Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
J. R. MACHADO-SILVA*
Affiliation:
Laboratório de Helmintologia Romero Lascasas Porto, Disciplina de Parasitologia, Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ 20550-170, Brasil
*
*Corresponding author: Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, UERJ, Av. Professor Manoel de Abreu, 444, 5° andar, 20550-170, Rio de Janeiro, RJ, Brasil. Tel: +55 21 2868 8048. E-mail: [email protected].
Get access Rights & Permissions [Opens in a new window]

Summary

This study aimed to investigate myocardial injuries in mice infected with Schistosoma mansoni and fed a high-fat chow. Sections of myocardial tissue from S. mansoni-infected mice, and controls that had been killed 9 and 17 weeks post-infection, were stained with H&E and Picrosirius red. Histopathological examination, stereological design-based method (optical disector) and morphometry (vessels, cardiomyocytes and an amount of collagen) were used. Data were analysed using two-way ANOVA. Regardless of time of infection, myocardial tissue from the infected mice fed high-fat chow showed myocarditis characterized by a higher number of inflammatory foci, several areas displaying coagulation of cardiac fibres, a greater loss of cardiomyocytes and fibroblast proliferation than in the standard chow control. Comparing infected mice from acute and chronic infections, a higher cardiomyocyte hyperplasia (P < 0·0001) and higher amounts of collagen (P < 0·05) were observed than in standard chow control. In addition, all animals fed high-fat chow showed lower numerical density and total number of cardiomyocytes (P < 0·05), thicker vessel walls and narrowed luminal intramyocardial vessels (P > 0·05) than in the standard chow control. Altogether the data supported the view that a double burden has a synergistic deleterious effect on the myocardial tissue.

Keywords

schistosomiasis mansonihearthigh-fat chowhistopathologystereology
Type
Research Article
Information
Parasitology , Volume 139 , Issue 11 , September 2012 , pp. 1462 - 1470
Copyright
Copyright © Cambridge University Press 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Águila, M. B., Mandarim-De-Lacerda, C. A. and Apfel, M. I. (1998). Stereology of the myocardium in young and aged rats. Arquivos Brasileiros de Cardiologia 70, 105–109. doi: 10.1590/S0066-782X1998000200007.CrossRefGoogle Scholar
Águila, M. B. and Mandarim-De-Lacerda, C. A. (2001). Blood pressure, ventricular volume and number of cardiomyocyte nuclei in rats fed for 12 months on chows differing in fat composition. Mechanisms of Ageing and Development 122, 77–88. doi: 10.1016/S0047-6374(00)00215-3.CrossRefGoogle Scholar
Águila, M. B. and Mandarim-De-Lacerda, C. A. (2003). Heart and blood pressure adaptations in Wistar rats fed with different high-fat chows for 18 months. Nutrition 19, 347–352. doi: 10.1016/S0899-9007(02)00934-6.CrossRefGoogle Scholar
Alencar, A. C. M. B., Neves, R. H., Águila, M. B., Mandarim-De-Lacerda, C. A., Gomes, D. C. and Machado-Silva, J. R. (2009). High-fat chow has a prominent effect upon the course of chronic schistosomiasis mansoni in mice. Memórias do Instituto Oswaldo Cruz 104, 608–613. doi: 10.1590/S0074-02762009000400013.CrossRefGoogle Scholar
Baptista, A. P. and Andrade, Z. A. (2005). Angiogenesis and schistosomal granuloma formation. Memórias do Instituto Oswaldo Cruz 100, 183–185. doi: 10.1590/S0074-02762005000200012.CrossRefGoogle ScholarPubMed
Barros, L. A., Costa-Silva, M., Biolchini, C. L., Neves, R. H. and Machado-Silva, J. R. (2009). Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein chow. Brazilian Journal of Medical and Biological Research 42, 812–815. doi: 10.1590/S0100-879X2009000900007.CrossRefGoogle Scholar
Borojevic, R. (1992). Experimental murine schistosomiasis mansoni: establishment of the chronic phase of the disease. Memórias do Instituto Oswaldo Cruz 87(Suppl. 4), 171–174. doi: 10.1590/S0074-02761992000800026.CrossRefGoogle ScholarPubMed
Boros, D. L. (1989). Immunopathology of Schistosoma mansoni infection. Clinical Microbiology Reviews 2, 250–269. doi: 10.1128/CMR.2.3.250.CrossRefGoogle ScholarPubMed
Carneiro, R. C. B., Santos, A. L. S., Brant, L. C. C., Rabelo, F. T., Ligeiro, C. M., Barcelos, I. P., Silva, V. B., Silva, V. S. X. and Nunes, M. C. P. (2011). Endomyocardial fibrosis associated with mansoni schistosomiasis. Revista da Sociedade Brasileira de Medicina Tropical 44, 644–645. doi: 10.1590/S0037-86822011000500026.CrossRefGoogle ScholarPubMed
Chisty, M. M., Nargis, M., Sato, H., Inaba, T. and Kamiya, H. (1999). Occurrence of myocarditis in rodents infected with Schistosoma mansoni. The Southeast Asian Journal of Tropical Medicine and Public Health 30, 556–561.Google ScholarPubMed
Chitsulo, L., Engels, D., Montresor, A. and Savioli, L. (2000). The global status of schistosomiasis and its control. Acta Tropica 77, 41–51. doi: 10.1016/S0001-706X(00)00122-4.CrossRefGoogle ScholarPubMed
De Barros Alencar, A. C., Neves, R. H., De Oliveira, A. V., Machado-Silva, J. R. (2012). Changes in the small intestine of Schistosoma mansoni-infected mice fed a high-fat diet. Parasitology 139, 716–725. doi: 10.1017/S0031182011002307.CrossRefGoogle Scholar
Doenhoff, M. J., Stanley, R. G., Griffiths, K. and Jackson, C. L. (2002). An anti-atherogenic effect of Schistosoma mansoni infections in mice associated with a parasite-induced lowering of blood total cholesterol. Parasitology 125, 415–421. doi: 10.1017/S0031182002002275.CrossRefGoogle ScholarPubMed
Epelboin, L., Jauréguiberry, S., Estève, J. B., Danis, M., Komajda, M., Bricaire, F. and Caumes, E. (2010). Myocarditis during acute schistosomiasis in two travelers. The American Journal of Tropical Medicine and Hygiene 82, 365–367. doi: 10.4269/ajtmh.2010.09-0084.CrossRefGoogle ScholarPubMed
Fernandes-Santos, C., de Souza Mendonça, L. and Mandarim-de-Lacerda, C. A. (2009). Favorable cardiac and aortic remodeling in olmesartan-treated spontaneously hypertensive rats. Heart Vessels 24, 219–227. doi: 10.1007/s00380-008-1104-3.CrossRefGoogle ScholarPubMed
Gryseels, B., Polman, K., Clerinx, J. and Kestens, L. (2006). Human schistosomiasis. Lancet 368, 1106–1118. doi: 10.1016/S0140-6736(06)69440-3.CrossRefGoogle ScholarPubMed
Kolosionek, E., Graham, B. B., Tuder, R. M., Butrous, G. (2011). Pulmonary vascular disease associated with parasitic infection – the role of schistosomiasis. Clinical Microbiology and Infection 17, 15–24. doi: 10.1111/j.1469-0691.2010.03308.x.CrossRefGoogle ScholarPubMed
La Flamme, A. C., Harvie, M., Kenwright, D., Cameron, K., Rawlence, N., Low, Y. S. and McKenzie, S. (2007). Chronic exposure to schistosome eggs reduces serum cholesterol but has no effect on atherosclerotic lesion development. Parasite Immunology 29, 259–266. doi: 10.1111/j.1365-3024.2007.00942.x.CrossRefGoogle ScholarPubMed
Lambertucci, J. R. (2010). Acute schistosomiasis mansoni: revisited and reconsidered. Memórias do Instituto Oswaldo Cruz 105, 422–435. doi: 10.1590/S0074-02762010000400012.CrossRefGoogle ScholarPubMed
Lapa, M., Dias, B., Jardim, C., Fernandes, C. J., Dourado, P. M., Figueiredo, M., Farias, A., Tsutsui, J., Terra-Filho, M., Humbert, M. and Souza, R. (2009). Cardiopulmonary manifestations of hepatosplenic schistosomiasis. Circulation 119, 1518–1523. doi: 10.1161/CIRCULATIONAHA.108.803221.CrossRefGoogle ScholarPubMed
Machado-Silva, J. R., Neves, R. H., Mota, E. C. and Mandarim-de-Lacerda, C. A. (2010). Hepatic stereology of acute schistosomiasis mansoni infection in C57BL/10 and CBA mice. Revista de Patologia Tropical 39, 211–219. doi: 10.5216/rpt.v39i3.12208.Google Scholar
Mandarim-De-Lacerda, C. A. (2003). Stereological tools in biomedical research. Anais da Academia Brasileira de Ciências 75, 469–486. doi: 10.1590/S0001-37652003000400006.CrossRefGoogle ScholarPubMed
Medeiros, F. J., Mothé, C. G., Águila, M. B. and Mandarim-De-Lacerda, C. A. (2005). Long-term intake of edible oils benefits blood pressure and myocardial structure in spontaneously hypertensive rat (SHR) and streptozotocin diabetic SHR. Prostaglandins & Other Lipid Mediators 78, 231–248. doi: 10.1016/j.prostaglandins.2005.09.001.CrossRefGoogle ScholarPubMed
Medeiros, M. V. and Andrade, Z. A. (1986). The importance of collateral portal veins for resistance to schistosomiasis in mice. Brazilian Journal of Medical and Biological Research 19, 85–88.Google ScholarPubMed
Neves, R. H., Alencar, A. C., Águila, M. B., Mandarim-De-Lacerda, C. A., Machado-Silva, J. R. and Gomes, D. C. (2006). Hepatic stereology of Schistosomiasis mansoni infected-mice fed a high-fat chow. Memórias do Instituto Oswaldo Cruz 101, 253–260. doi: 10.1590/S0074-02762006000900039.CrossRefGoogle Scholar
Okere, I. C., Chandler, M. P., McElfresh, T. A., Rennison, J. H., Sharov, V., Sabbah, H. N., Tserng, K. Y., Hoit, B. D., Ernsberger, P., Young, M. E. and Stanley, W. C. (2006). Differential effects of saturated and unsaturated fatty acid chows on cardiomyocyte apoptosis, adipose distribution, and serum leptin. American Journal of Physiology – Heart and Circulatory Physiology 291, H38–H44. doi: 10.1152/ajpheart.01295.2005.CrossRefGoogle Scholar
Palaniyandi, S. S., Sun, L., Ferreira, J. C. and Mochly-Rosen, D. (2009). Protein kinase C in heart failure: a therapeutic target? Cardiovascular Research 82, 229–239. doi: 10.1093/cvr/cvp001.CrossRefGoogle ScholarPubMed
Pinheiro, A. R., Cunha, A. R., Águila, M. B. and Mandarim-De-Lacerda, C. A. (2007). Beneficial effects of physical exercise on hypertension and cardiovascular adverse remodeling of chow-induced obese rats. Nutrition, Metabolism & Cardiovascular Diseases 17, 365–375. doi: 10.1016/j.numecd.2006.01.009.CrossRefGoogle Scholar
Ramanampamonjy, R. M., Razafimahefa, S. H., Rajaonarivelo, P. and Rajaona, H. R. (2007). Portopulmonary hypertension due to schistosomiasis in two Malagasy patients. Bulletin de la Société de Pathologie Exotique 100, 28–29.Google ScholarPubMed
Rashwan, M. A., Ayman, M., Ashour, S., Hassanin, M. M. and Zeina, A. A. (1995). Endomyocardial fibrosis in Egypt: an illustrated review. British Heart Journal 73, 284–289.CrossRefGoogle ScholarPubMed
Rich, L. and Whittaker, P. (2005). Collagen and picrosirius red staining: a polarized light assessment of fibrillar hue and spatial distribution. Brazilian Journal of Morphological Science 22, 97–104.Google Scholar
Scherle, W. (1970). A simple method for volumetry of organs in quantitative stereology. Mikroskopie 26, 57–60.Google ScholarPubMed
Silva, L. M., Ribeiro-Dos-Santos, R., Soares, M. B. and Andrade, Z. A. (2006). Characterization of the vascular changes in schistosomal portal (pipestem) fibrosis of mice. Acta Tropica 98, 34–42. doi: 10.1016/j.actatropica.2006.01.011.CrossRefGoogle ScholarPubMed
Sterio, D. C. (1984). The unbiased estimation of number and sizes of arbitrary particles using the disector. Journal of Microscopy 134, 127–136. doi: 10.1111/j.1365-2818.1984.tb02501.x.CrossRefGoogle ScholarPubMed
Tang, Y., Nyengaard, J. R., Andersen, J. B., Baandrup, U. and Gundersen, H. J. (2009). The application of stereological methods for estimating structural parameters in the human heart. The Anatomical Record – Advances in Integrative Anatomy and Evolutionary Biology 292, 1630–1647. doi: 10.1002/ar.20952.CrossRefGoogle ScholarPubMed
Taube, J. M. and Hutchins, G. M. (2008). Multifocal ischemic necroses of varying age (MINOVA): A distinctive form of atherosclerotic heart disease. Pathology, Research & Practice 204, 113–120. doi: 10.1016/j.prp.2007.08.011.CrossRefGoogle Scholar
Thone-Reineke, C., Kalk, P., Dorn, M., Klaus, S., Simon, K., Pfab, T., Godes, M., Persson, P., Unger, T. and Hocher, B. (2006). High-protein nutrition during pregnancy and lactation programs blood pressure, food efficiency, and body weight of the offspring in a sex-dependent manner. American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 291, R1025–R1030. doi: 10.1152/ajpregu.00898.2005.CrossRefGoogle Scholar
Victor, E. G., Lira, V., Arruda, A., Monteiro, I. and Lima, R. (1996). Cardiac granulomas due to Schistosoma eggs and endomyocardial fibrosis. Arquivos Brasileiros de Cardiologia 67, 259–261.Google ScholarPubMed
Warren, K. S. (1964). Experimental pulmonary schistosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 58, 228–233. doi: 10.1016/0035-9203(64)90034-3.CrossRefGoogle ScholarPubMed
Wulfsohn, D., Nyengaard, J. R. and Tang, Y. (2004). Postnatal growth of cardiomyocytes in the left ventricle of the rat. The Anatomical Record Part A – Discoveries in Molecular, Cellular and Evolucionary Biology 277, 236–247. doi: 10.1002/ar.a.20009.CrossRefGoogle ScholarPubMed
Zar, J. H. (1999). Biostatical Analysis, 4th Edn. Prentice Hall, Upper Saddle River, NJ, USA.Google Scholar
Zornoff, L. A., Matsubara, B. B., Matsubara, L. S., Minicucci, M. F., Azevedo, P. S., Camapanha, A. O. and Paiva, S. A. (2006). Cigarette smoke exposure intensifies ventricular remodeling process following myocardial infarction. Arquivos Brasileiros de Cardiologia 86, 276–282. doi: 10.1590/S0066-782X2006000400007.Google ScholarPubMed