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The effects of drugs on worm expulsion in the Nippostrongylus brasiliensis infected rat: a discussion of the interpretation of drug action

Published online by Cambridge University Press:  06 April 2009

R. Keller  and
Bridget M. Ogilvie
R. Keller*
Affiliation:
Immunobiology Research Group University of Zurich
Bridget M. Ogilvie
Affiliation:
National Institute for Medical Research Mill Hill, London
*
*Supported by the Swiss National Foundation for Scientific Research (Grant 5200.3).
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Extract

In rats treated with compound 48/80 or histamine, worm expulsion was inhibited. Treatment with a histidine decarboxylase inhibitor accelerated worm expulsion. Treatment with compound 48/80 elevates histamine and histidine decarboxylase levels and reduces circulating reagin titres. These results show that histamine is not responsible for worm expulsion.

Compounds such as isoprenaline and theophylline which increase cellular levels of cyclic 3′,5-AMP, prevented worm expulsion.

It is concluded that the evidence that amines are involved in worm expulsion needs reassessing and that cellular release mechanisms in general may be affected by drugs thought to act solely on amine release. In particular, the release of effector substances from sensitized lymphocytes on contact with antigen may be affected by these treatments.

The skilful technical assistance of Miss R. Keist and Miss I. Beeger is gratefully acknowledged. We thank Mr H. Berchtold, Biostatistisches Zentrum der Universität Zürich, for the statistical evaluation of the data.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 2 , April 1972 , pp. 217 - 227
Copyright
Copyright © Cambridge University Press 1972

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References

REFERENCES

Assem, E. S. K. & Schild, H. O. (1969). Inhibition by sympathico mimetic amines of histamine release induced by antigen in passively sensitized human lung. Nature, London 224, 1028–9.Google Scholar
Berde, B., Doepfner, W. & Cerletti, A. (1960). Ueber die Wirkungsdauer einiger Serotoninantogonisten. Helvetica physiologica et pharmacologica Acta 18, 537–44.Google Scholar
Bovet, D., Horclois, R. & Walthert, F. (1944). Propriétés antihistaminiques de la N-p-méthoxybenzyl-N-diméthylaminoéthyl α amino-pyridine. Comptes Rendus des Séances de la Société de Biologie 138, 99–100.Google Scholar
Butcher, R. W. & Sutherland, E. W. (1962). Adenosine 3′,5′-phosphate in biological materials. I. Purification and properties of cyclic 3′,5′-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3′,5′-phosphate in human urine. Journal of Biological Chemistry 237, 1244–50.Google Scholar
Connan, R. M. (1970). The effect of host lactation on the self-cure of Nippostrongylus brasiliensis in rats. Parasitology 61, 27–33.CrossRefGoogle Scholar
Dineen, J. K. & Kelly, J. D. (1971). The suppression of rejection of Nippostrongylus brasiliensis in lactating rats: the nature of the immunological defect. Immunology (in the Press).Google Scholar
Falke, D. & Netter, K. J. (1969). Die Hemmung der Riesenzellbildung durch das sogen-annte Compound 48/80 nach der Infektion mit dem Herpesvirus hominis. I. Die Wirkung auf die Virussynthese. Archiv für die gesamte Virusforschung 28, 308–24.CrossRefGoogle Scholar
Ishizaka, T., Ishizaka, K. & Lichtenstein, L. M. (1971). Antibody-induced histamine release and degranulation of human basophil leukocytes. Federation Proceedings 30, 654.Google Scholar
Jones, V. E. & Ogilvie, B. M. (1971). Protective immunity to Nippostrongylus brasiliensis: the sequence of events which expels worms from the rat intestine. Immunology, 20, 549–61.Google ScholarPubMed
Keller, R. (1970 a). Immune reactions to Nippostrongylus brasiliensis in the rat. I. Characteristics of primary and secondary immune response in vivo. International Archives of Allergy and Applied Immunology 37, 197–215.CrossRefGoogle Scholar
Keller, R. (1970 b). On the mechanism of expulsion of helminths. Clinical and experimental Immunology 6, 207–10.Google ScholarPubMed
Keller, R. (1970 c). Unpublished data.Google Scholar
Keller, R. (1971). Nippostrongylus brasiliensis in the rat: Failure to relate intestinal histamine and mast cell levels with worm expulsion. Parasitology 63, 473–81.CrossRefGoogle ScholarPubMed
Keller, R. & Jones, V. E. (1971). Immunological analysis of the primary and secondary reagin response to Nippostrongylus brasiliensis in the rat. Immunology 21, 565–74.Google ScholarPubMed
Kelly, J. D. & Dineen, J. K. (1971). The suppression of rejection of Nippostrongylus brasiliensis in the rat by promethazine hydrochloride: the site of immunological impairment. Submitted to Immunology.Google Scholar
Lichtenstein, L. M. & Margolis, S. (1968). Histamine release in vitro: Inhibition by cate-cholamines and methylxanthines. Science 161, 902–3.Google Scholar
Majno, G., Palade, G. E. & Schoefl, G. I. (1961). Studies on inflammation. II. The site of action of histamine and serotonin along the vascular tree: a topographic study. Journal of Biophysical and Biochemical Cytology 11, 607–26.CrossRefGoogle Scholar
Murad, F., Chi, Y.-M., Rall, W. T. & Sutherland, E. W. (1962). Adenylcyclase. III. The effect of catecholamines and choline esters on the formation of adenosine 3′,5′-phosphate by preparations from cardiac muscle and liver. Journal of Biological Chemistry 237, 1233–8.CrossRefGoogle Scholar
Murray, M., Miller, H. R. P. & Jarrett, W. F. A. (1968). The globule leukocyte and its derivation from the subepithelial mast cell. Laboratory Investigation 19, 222–34.Google Scholar
Murray, M., Miller, H. R. P., Sanford, J. & Jarrett, W. F. H. (1971). 5-Hydroxytrypta-mine in intestinal immunological reactions. Its relationship to mast cell activity and worm expulsion in rats infected with Nippostrongylus brasiliensis. International Archives of Allergy and Applied Immunology 40, 236–47.CrossRefGoogle Scholar
Ogilvie, B. M. (1965 a). Role of adult worms in immunity of rats to Nippostrongylus brasiliensis. Parasitology 55, 325–35.CrossRefGoogle ScholarPubMed
Ogilvie, B. M. (1965 b). Use of cortisone derivatives to inhibit resistance to Nippostrongylus brasiliensis and to study the fate of parasites in resistant hosts. Parasitology 55, 723–30.Google Scholar
Ogilvie, B. M. & Hockley, D. J. (1968). Effects of immunity on Nippostrongylus brasiliensis adult worms: reversible and irreversible changes in infectivity, reproduction, and morphology. Journal of Parasitology 54, 1073–84.Google Scholar
Ogilvie, B. M. & Jones, V. E. (1971). Nippostrongylus brasiliensis in the rat. A review of mmunity and the host/parasite relationship. Experimental Parasitology 29, 138–77.Google Scholar
Reilly, M. A. & Schayer, R. W. (1968). Further studies on the histidine-histamine relationship in vivo: effects of endotoxin and of histidine decarboxylase inhibitors. British Journal of Pharmacology 34, 551–63.CrossRefGoogle ScholarPubMed
Riley, J. F. & West, G. B. (1955). Tissue mast cells. Studies with a histamine-liberator of low toxicity (compound 48/80). Journal of Pathology and Bacteriology 69, 269–82.CrossRefGoogle ScholarPubMed
Roth, E., Noltenius, H. & Oehlert, W. (1963). Autoradiographische Untersuchungen zur DNS-Neubildung und zum Sulfateinbau in Gewebemastzellen. Frankfurter Zeitschrift für Pathologie 73, 40–51.Google Scholar
Schayer, R. W., Rothschild, Z. & Bizony, P. (1959). Increase in histidine decarboxylase activity of rat skin following treatment with compound 48/80. American Journal of Physiology 196, 295–8.CrossRefGoogle ScholarPubMed
Stone, C. A., Wenger, H. C., Ludden, C. T., Stavorski, J. M. & Ross, C. A. (1961). Antiserotonin-antihistaminic properties of cyproheptadine. Journal of Pharmacology and experimental Therapeutics 131, 73–84.Google Scholar
Stormorken, H. (1969). The release reaction of secretion. Scandinavian Journal of Haematology, Supplement 9, 3–24.CrossRefGoogle ScholarPubMed
Uvnäs, B. & Wold, J. K. (1967). Isolation of a mast cell degranulating polypeptide from Ascaris suis. Acta physiologica scandinavica 70, 269–76.Google Scholar