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The effect of substrates and inhibitors on the rate of respiration of the infective larvae of Strongyloides ratti Sandground, 1925

Published online by Cambridge University Press:  06 April 2009

J. Barrett
J. Barrett
Affiliation:
The Molteno Institute, University of Cambridge*
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Extract

The infective larvae of S. ratti were non-feeding and none of a wide variety of possible substrates had any effect on the respiratory rate of intact worms. Sodium succinate increased the oxygen uptake of larval homogenates.

The cytochrome chain inhibitors, sodium cyanide, sodium azide and urethane all reversibly inhibited the respiration of the infective larvae. The inhibition caused by carbon monoxide was photo-reversible and 2,4-dinitrophenol inhibited respiration rather than stimulated it. Methylene blue increased the oxygen uptake of homogenates, but had no effect on the intact larva. Iodoacetamide and sodium arsenite irreversibly inhibited respiration, whilst sodium fluoride, sodium pyrophosphate and sodium malonate were without effect on the intact larvae.

This work was carried out during the tenure of a Medical Research Council Scholarship.

Type
Research Article
Information
Parasitology , Volume 59 , Issue 2 , May 1969 , pp. 343 - 347
Copyright
Copyright © Cambridge University Press 1969

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References

REFERENCES

Abadie, S. H. (1963). The life cycle of Strongyloides ratti. J. Parasit. 49, 241–8.CrossRefGoogle ScholarPubMed
Barrett, J. (1968). The effect of temperature on the development and survival of the infective larvae of Strongyloides ratti Sandground, 1925. Parasitology 58, 641–51.CrossRefGoogle ScholarPubMed
von Brand, T. (1966). Biochemistry of Parasites, 1st ed.New York and London: Academic Press.Google Scholar
Fernando, M. A. (1963). Metabolism of hookworms. I. Observations on the oxidative metabolism of free-living, third-stage larvae of Necator americanus. Expl Parasit. 13, 90–7.CrossRefGoogle ScholarPubMed
Fernando, M. A. & Wong, H. A. (1964). Metabolism of hookworms. II. Glucose metabolism and glycogen synthesis in adult female Ancylostoma caninum. Expl Parasit. 15, 284–92.CrossRefGoogle ScholarPubMed
Giovannola, A. (1936). Energy and food reserves in the development of nematodes. J. Parasit. 22, 207–18.CrossRefGoogle Scholar
Goldberg, E. (1957). Studies on the intermediary metabolism of Trichinella spiralis. Expl Parasit. 6, 367–82.CrossRefGoogle ScholarPubMed
Karpiak, S. E., Kozar, Z., Krzyzanowski, M. & Kozar, M. (1965). Effect of some organic acids on the respiration of Trichinella spiralis larvae. Acta parasit. pol. 13, 265–70.Google Scholar
Kozar, Z., Karpiak, S. E., Krzyzanowski, M. & Kozar, M. (1965). Observations on the carbohydrate metabolism of Trichinella spiralis muscular larvae. Acta parasit. pol. 13, 259–64.Google Scholar
Looss, A. (1911). The anatomy and life history of Agchylostoma duodenale Dub. Rec. Sch. Med., Cairo 4, 167–607.Google Scholar
Peel, J. L. (1963). The catalysis of the auto-oxidation of 2-mercaptoethanol and other thiols by vitamin B12 derivatives. Biochem. J. 88, 296–38.CrossRefGoogle ScholarPubMed
Roberts, L. S. & Fairbairn, D. (1965). Metabolic studies on adult Nippostrongylus brasi-liensis (Nematoda: Trichostronglyoidea). J. Parasit. 51, 129–38.CrossRefGoogle Scholar
Schwabe, C. W. (1957). Observations on the respiration of the free-living and parasitic Nippostrongylus muris larvae. Am. J. Hyg. 65, 325–37.Google ScholarPubMed
Silver, I. A. (1963). A simple micro-cathode for measuring pO2 in gas or fluid. Med. Electron. biol. Engng 1, 547–51.CrossRefGoogle Scholar
Stannard, J. N., McCoy, O. R. & Latchford, W. B. (1938). Studies on the metabolism of Trichinella spiralis larvae. Am. J. Hyg. 27, 666–82.Google Scholar
Warren, L. G., Guevara, A. & Patzrek, D. (1962). Respiration and carbohydrate metabolism of Ancylostoma caninum: Effect of carbohydrates, acid intermediates and electron transport inhibitors. J. Parasit. 48 (Suppl.) 25–6. (Abstr.)Google Scholar