Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T04:32:21.433Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of selection pressure for thiabendazole resistance on fitness of Haemonchus contortus in sheep

Published online by Cambridge University Press:  06 April 2009

N. Maingi ,
M. E. Scott  and
R. K. Prichard
N. Maingi
Affiliation:
Institute of Parasitotogy, Macdonald College of McGill University, 21 111 Lakeshore Road, Ste-Anne de Bellevue, Quebec H9X 1CO, Canada
M. E. Scott
Affiliation:
Institute of Parasitotogy, Macdonald College of McGill University, 21 111 Lakeshore Road, Ste-Anne de Bellevue, Quebec H9X 1CO, Canada
R. K. Prichard
Affiliation:
Institute of Parasitotogy, Macdonald College of McGill University, 21 111 Lakeshore Road, Ste-Anne de Bellevue, Quebec H9X 1CO, Canada
Get access Rights & Permissions [Opens in a new window]

Summary

A Strain ofHaemonchus contortusshowing moderate levels of resistance to thiabendazole (TBZ) (egg hatch assay LC50 2.1 μM) was subjected, in alternating sequence, to 5 in vitro and 4 in vivo selections with TBZ. This resulted in a significant increase in resistance (egg hatch assay LC50 2.8 μM). The isolates obtained after each combined in vitro and in vivo selection were compared in terms of egg hatch assay, establishment, net egg output, per capita egg output and degree of pathology caused in infected hosts. The increase in resistance was accompanied by a significant increase in larval establishment sheep (22 %) and was associated with more severe pathology, higher egg count and worm recovery 3 months post- infection.

Keywords

thaibendazole resistanceHaemonchus contortusestablishmentegg productionpathogenesisfitness selection
Type
Research Article
Information
Parasitology , Volume 100 , Issue 2 , April 1990 , pp. 327 - 335
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anderson, R. M. & May, R. M. (1982). Population dynamics of human helminth infections: control by chemotherapy. Nature, London 297, 557–63.CrossRefGoogle ScholarPubMed
Borgsteede, F. H. M. & Couwenberg, T. (1987). Changes in LC50 in an in vitro egg development assay during the patent period of Haemonchus contortus in sheep. Research in Veterinary Science 42, 413–14.CrossRefGoogle Scholar
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye. Analytical Biochemistry 72, 248–54.CrossRefGoogle ScholarPubMed
Clarke, C. M. & McKenzie, J. A. (1987). Developmental stability of insecticide resistant phenotypes in blowfly; a result of canalizing natural selection. Nature, London 325, 345–6.CrossRefGoogle Scholar
Coles, H. E. (1974). Veterinary Clinical Pathology, 2nd Edn.Philadelphia: Saunders & Co.Google Scholar
Dash, K. M. (1986). Multiple anthelmintic resistance in Trichostrongylus colubriformis. Australian Veterinary Journal 63, 45–7.CrossRefGoogle ScholarPubMed
Finney, D. J. (1971). Probit Analysis, 3rd Edn.Cambridge: Cambridge University Press.Google Scholar
Hall, C. A., Ritchie, L. & Kelly, J. D. (1982). Effect of removing anthelmintic selection pressure on the benzimidazole resistant status of Haemonchus contortus and Trichostrongylus colubriformis in sheep. Research in Veterinary Science 34, 54–7.CrossRefGoogle Scholar
Kelly, J. D. & Hall, C. A. (1979). Resistance of animal helminths to anthelmintics. Advances in Pharmacological Chemotherapy 16, 89–128.CrossRefGoogle ScholarPubMed
Kelly, J. D., Hall, C. A., Whitlock, H. V., Thompson, H. C., Campbell, N. J. & Martin, I. C. A. (1977). The effect of route of administration on the anthelmintic efficacy of benzimidazole anthelmintics in sheep infected with Strains of Haemonchus contortus and Trichostronglylus colubriformis resistant or susceptible to thiabendazole. Research in Veterinary Science 22, 161–8.CrossRefGoogle ScholarPubMed
Kelly, J. D., Whitlock, H. V., Thompson, H. C., Hall, C. A., Martin, I. C. A. & Lejambre, L. F. (1978). Physiological characteristics of the free-living and parasitic stages of Strains of Haemonchus contortus susceptible or resistant to benzimidazole anthelmintics. Research in Veterinary Science 25, 376–85.CrossRefGoogle ScholarPubMed
Lejambe, L. F. (1976). Egg hatch as an in vitro assay of thiabendazole resistance in nematodes. Veterinary Parasitology 2, 385–91.CrossRefGoogle Scholar
Lejambre, L. F., Martin, P. J. & Jarrett, R. G. (1982). Comparison of changes in resistance of Haemonchus contortus eggs following withdrawal of thiabendazole selection. Research in Veterinary Science 32 39–43.CrossRefGoogle Scholar
Lejambre, L. F., Southcott, W. H. & Dash, K. M. (1978). Effectiveness of broad spectrum anthelmintics against selected strains of Trichostrongylus colubriformis. Australian Veterinary Journal 54, 570–4.CrossRefGoogle Scholar
Maclean, J. M., Lewis, D. & Holmes, P. H. (1987). The pathogenesis of benzimidazole-resistant and benzimidazole-susceptible strains of Trichostrongylus colubriformis in the Mongolian gerbil (Meriones unguiculatus). Journal of Helminthology 61, 179–89.CrossRefGoogle ScholarPubMed
Martin, P. J. (1986). Development and control of resistance to anthelmintics. In Parasitology Quo Vadit? Proceedings of the 6th International Congress of Parasitology (ed. Howell, M. J.), pp. 493–501. (Australian Academy of Sciences).Google Scholar
Martin, P. J., Anderson, N., Brown, T. H. & Miller, D. W. (1988). Changes in resistance of Ostertagia spp. to thiabendazole following natural selection or treatment with levamisole. International Journal for Parasitology 18, 333–40.CrossRefGoogle ScholarPubMed
Simpkin, K. C. & Coles, G. C. (1978). Instability in benzimidazole resistance in nematode eggs. Research in Veterinary Science 25, 249–50.CrossRefGoogle ScholarPubMed
Waller, P. J. & Prichard, R. K. (1986). Drug resistance in nematodes. In Chemotherapy of Parasitic Diseases (ed. Campbell, W. G. & Robert, S.), pp. 339–62. New York: Plenum Publishing Corporation.CrossRefGoogle Scholar
Whitlock, H. V. (1956). Some modifications of the McMaster helminth egg counting technique and apparatus. Australian Veterinary Journal 32, 177–80.Google Scholar